Limonene Synthase: Detailed information on the kinetic values obtained from the literature

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Parameter Value Units Description References
Enzyme amount 1-10 µg An excerpt from Alonso 1992, Materials and Methods section [1]
[GPP] 10 µM An excerpt from Alonso 1992, Materials and Methods section [1]
Specific enzyme activity 19 µmol/h/mg An excerpt from Alonso 1992 [1]
Kcat 0.3 s-1 An excerpt from Alonso 1992 [1]
Kcat 0.020 s-1 An excerpt from Williams 1998, Result section [2]
[GPP] 10 µM An excerpt from Günnewich 2006 [3]
Enzyme Amount (Possibly) 1.25 µg Possible it is referring to enzyme amount used for CsTPS1 (limonene producing terpene synthase). 
An excerpt from Günnewich 2006
[3]
KmGPP 6.8 µM Km and Kcat value for CsTPS1 (limonene producing terpene synthase)

An excerpt from Günnewich 2006

[3]
KmGPP 16 µM An excerpt from Williams 1998, results section [2]
Kcat 0.082 s-1 Km and Kcat value for CsTPS1 (limonene producing terpene synthase)

An excerpt from Günnewich 2006

[3]
[GPP] 25 µM GPP concentration used, for preprotein (?) expressed in E.coli.

An excerpt from Williams 1998, Materials and Methods section

[2]
Enzyme amount 150 µg Purified (-)-4S-limonene synthase amount.

An excerpt from Williams 1998, Materials and Methods section

[2]

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References

  1. ↑ 1.0 1.1 1.2 1.3 Alonso et. al. 1992. "Purification of 4S-Limonene Synthase, a Monoterpene Cyclase from the Glandular Trichomes of Peppermint (Mentha x piperita) and Spearmint (Mentha spicata)", The journal of Biological Chemistry, 267(11):7582-7587
  2. ↑ 2.0 2.1 2.2 2.3 Cite error: Invalid <ref> tag; name "Williams1998" defined multiple times with different content Cite error: Invalid <ref> tag; name "Williams1998" defined multiple times with different content
  3. ↑ 3.0 3.1 3.2 3.3 Günnewich, N. et. al. 2006. "Functional expression and characterization of trichome-specific (-)-limonene synthase and (+)-α-pinene synthase from Cannabis sativa", Natural Product Communications, 0(0):1-10 Cite error: Invalid <ref> tag; name "Gunnewich2006" defined multiple times with different content Cite error: Invalid <ref> tag; name "Gunnewich2006" defined multiple times with different content Cite error: Invalid <ref> tag; name "Gunnewich2006" defined multiple times with different content
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