Enolase
Enolase, also known as phosphopyruvate hydratase, catalysis the conversion of 2-phosphoglycerate (2-PG) to phosphoenolpyruvate (PEP). This is the penultimate step of glycolysis.
Contents
Chemical equation
![2PG \rightleftharpoons PEP](/wiki/images/math/e/3/6/e36e43bdcf85d82cfe2a632b8567be12.png)
Rate equation
Mono-substrate reversible Michaelis-Menten equation is used. [1]
![\frac{V_{mf}\frac{[2PG]}{K_{2PG}}-V_{mr}\frac{[PEP]}{K_{PEP}}}{1 + \frac{[2PG]}{K_{2PG}} + \frac{[PEP]}{K_{PEP}}}](/wiki/images/math/6/a/9/6a9ca37a2fd76b4eb56a1e6a5cfb67d0.png)
Modified rate law to take Thermodynamic constraint into consideration
![\frac{V_{mf}\frac{[2PG]}{K_{2PG}} \left( 1 -\frac{[PEP]}{K_{eq} [2PG]} \right)}{1 + \frac{[2PG]}{K_{2PG}} + \frac{[PEP]}{K_{PEP}}}](/wiki/images/math/b/3/3/b33ffe063d57a3aa7d01ca57ebd5e7dc.png)
Parameter values
Parameter | Value | Units | Organism | Remarks |
---|---|---|---|---|
![]() |
0.34 [2] | ![]() |
HeLa cell line | |
![]() |
0.38[1] | ![]() | ||
![]() |
0.038[1] | mM | ||
![]() |
0.06[1] | mM |
Parameters with uncertainty
- Three values for
is collected. The values are 0.20 [3], 0.199 [3], 0.038 [1]. The mean and std. dev. is
- Similarly for
three reported values are 0.58, 0.702, 0.06. The uncertainty is then
.
- In Pietkiewicz et. al. (2009) [3]
is reported as 1.4
and Marín-Hernández et. al. (2011) [1] reported it to be 0.4. The mean and the std. dev. calculated from these two values are
.
Parameter | Value | Units | Organism | Remarks |
---|---|---|---|---|
![]() |
![]() |
![]() |
HeLa cell line | |
![]() |
![]() |
![]() | ||
![]() |
![]() |
mM | Human muscle | |
![]() |
![]() |
mM | Human muscle |
References
- ↑ 1.0 1.1 1.2 1.3 1.4 1.5 Marín-Hernández A, Gallardo-Pérez JC, Rodríguez-Enríquez S et al (2011) Modeling cancer glycolysis. Biochim Biophys Acta 1807:755–767 (doi)
- ↑ 2.0 2.1 Marín-Hernández A , Rodríguez-Enríquez S, Vital-González P A, et al. (2006). Determining and understanding the control of glycolysis in fast-growth tumor cells. Flux control by an over-expressed but strongly product-inhibited hexokinase. FEBS J., 273 , pp. 1975–1988(doi)
- ↑ 3.0 3.1 3.2 Pietkiewicz, J., Gamian, A., Staniszewska, M., & Danielewicz, R. (2009), Inhibition of human muscle-specific enolase by methylglyoxal and irreversible formation of advanced glycation end products, Journal of Enzyme Inhibition and Medicinal Chemistry, 24, 356–364