Glycogen phosphorylase
Glycogen phosphorylase (GP) is a dimeric enzyme that catalyses the reaction in which the terminal glucose residue from a glycogen chain is phosphorylated and cleaved from the chain, releasing it as Glc1P.
Contents
Chemical equation
Rate equation
MWC model was used to formualte the rate law. [1]
An alternative rate equation without considering the allosteric regulation is given as [2]
Parameter values
Parameter | Value | Units | Organism | Remarks |
---|---|---|---|---|
2 | Dimensionless | Recombinant, human muscle | ||
50 | ||||
2.08 | mM | |||
4.32 | mM | |||
41.53 | mM | |||
0.67 | mM | |||
82.02 | mM | |||
27.92 | mM | |||
mM | ||||
0.53 | mM | |||
3.9 | mM | |||
7.42 | mM | |||
0.56 | mM | |||
0.27 | mM | |||
5.93 | Dimensionless | |||
34741 | Dimensionless |
The parameter for the alternative equation are
Parameter | Value | Units | Organism | Remarks |
---|---|---|---|---|
50 [1] | Rabbit | |||
15[2] | mM | |||
4 [2] | mM | |||
0.15 [2] | mM | |||
10.1 [2] | mM | |||
4.6 [2] | mM | |||
4.4 [2] | mM | |||
1.7 [2] | mM | |||
0.42 [2] | mM |
Parameters with uncertainty
Parameter | Value | Units | Organism | Remarks |
---|---|---|---|---|
2 | Dimensionless | Recombinant, human muscle | ||
mM | ||||
mM | ||||
mM | ||||
mM | ||||
mM | ||||
mM | ||||
mM | ||||
mM | ||||
mM | ||||
mM | ||||
mM | ||||
mM | ||||
Dimensionless | ||||
Dimensionless |
The parameter for the alternative equation are
- No uncertainty for and were reported. Therefore the uncertainty was calculated based on the highest uncertainty reported for all the values in the table below which is .
Parameter | Value | Units | Organism | Remarks |
---|---|---|---|---|
[1] | Rabbit | |||
mM | ||||
[3] | mM | |||
[3] | mM | |||
[3] | mM | |||
[3] | mM | |||
mM | ||||
[3] | mM | |||
Two values of 0.30, 0.42 are reported in [4][2]. Mean and Std. Dev. from these two values are taken. |
References
- ↑ 1.0 1.1 1.2 Palm, D.C. (2013). The regulatory design of glycogen metabolism in mammalian skeletal muscle (Ph.D.). University of Stellenbosch
- ↑ 2.0 2.1 2.2 2.3 2.4 2.5 2.6 2.7 2.8 2.9 Lambeth M.J. & Kushmerick M.J. (2002). A computational model for glycogenolysis in skeletal muscle. Ann Biomed Eng 30, 808–827
- ↑ 3.0 3.1 3.2 3.3 3.4 Gold, A. M., R. M. Johnson, and J. K. Tseng (1970), Kinetic mechanism of rabbit muscle glycogen phosphorylase a,J. Biol. Chem. 245:2564 –2572, 1970
- ↑ Principles of Metabolic Regulation, Book chapter