Lactate dehydrogenase
A dehydrogenase is an enzyme that transfers a hydride from one molecule to another. Lactate dehydrogenase catalyzes the conversion of pyruvate to lactate and back, as it converts NADH to NAD+ and back.
Contents
Chemical reactions
![NADH + PYR \rightleftharpoons Lactate_{in} + NAD^+](/wiki/images/math/e/1/e/e1eba3432a22de6d0161e8a0e3297261.png)
Rate equation
RAndom Bi-Bi reversible Michaelis-Menten equation is used. [1]
![\frac{V_{mf}\frac{[NADH][PYR]}{Km_{NADH} K_{PYR}} - V_{mr}\frac{[Lactate_{in}][NAD]}{Km_{Lactate_{in}} K_{NAD}}}{1 + \frac{[NADH]}{Km_{NADH}} + \frac{[PYR]}{Km_{PYR}} + \frac{[NADH][PYR]}{Km_{NADH} Km_{PYR}} + \frac{[Lactate_{in}][NAD]}{Km_{Lactate_{in}} Km_{NAD}} + \frac{[Lactate_{in}]}{Km_{Lactate_{in}}} + \frac{[PYR]}{Km_{PYR}} }](/wiki/images/math/4/b/2/4b2b29cf3850c4c61d11218132ecd486.png)
Prameter values
Parameter | Value | Units | Organism | Remarks |
---|---|---|---|---|
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3.4 [1] | ![]() |
HeLa cell line | |
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0.54 | ![]() |
HeLa cell line | |
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0.1 | mM | HeLa cell line | |
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4.7 | mM | Rat AS-30D hepatoma | |
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0.07 | mM | HeLa cell line | |
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0.002 | mM | HeLa cell line |
Parameters with uncertainty
- Mean and Std. Dev. for
has been reported in Table S3 for Marín-Hernández (2011) et. al. [1]. The Std. Dev. for
is calculated based on the same ratio for
.
Parameter | Value | Units | Organism | Remarks |
---|---|---|---|---|
![]() |
Failed to parse (Cannot store math image on filesystem.): 3.4 \pm 0.5 (3) [2] | ![]() |
HeLa cell line | |
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![]() |
![]() |
HeLa cell line | |
![]() |
0.1 | mM | HeLa cell line | |
![]() |
4.7 | mM | Rat AS-30D hepatoma | |
![]() |
0.07 | mM | HeLa cell line | |
![]() |
0.002 | mM | HeLa cell line |
References
- ↑ 1.0 1.1 1.2 Marín-Hernández A, Gallardo-Pérez JC, Rodríguez-Enríquez S et al (2011) Modeling cancer glycolysis. Biochim Biophys Acta 1807:755–767 (doi)
- ↑ Marín-Hernández A , Rodríguez-Enríquez S, Vital-González P A, et al. (2006). Determining and understanding the control of glycolysis in fast-growth tumor cells. Flux control by an over-expressed but strongly product-inhibited hexokinase. FEBS J., 273 , pp. 1975–1988(doi)