3-phosphoglycerate mutase

Phosphoglycerate mutase (PGAM) is an enzyme that catalyzes the internal transfer of a phosphate group from C-3 to C-2 which results in the conversion of 3-phosphoglycerate (3PG) to 2-phosphoglycerate (2PG).

Chemical equation

$3PG \rightleftharpoons 2PG$

Rate equation

Mono-substrate reversible Michaelis-Menten equation is used.

$\frac{V_{mf}\frac{[3PG]}{Km_{3PG}}-V_{mr}\frac{[2PG]}{Km_{2PG}}}{1 + \frac{[3PG]}{Km_{3PG}} + \frac{[2PG]}{Km_{2PG}}}$

Parameter values

Parameter	Value	Units	Organism	Remarks
$V_{mf}$	0.94	$\text{mM min}^{-1}$	HeLa cell line
$V_{mr}$	0.36	$\text{mM min}^{-1}$
$Km_{3PG}$	0.19	mM
$Km_{2PG}$	0.12	mM

Parameters with uncertainty

• Three reported values of $Km_{3PG}$ are considered. 0.19 , 0.5 and 0.4

Parameter	Value	Units	Organism	Remarks
$V_{mf}$	$1.4 \pm 1$	$\text{mM min}^{-1}$	HeLa cell line
$V_{mr}$	0.36	$\text{mM min}^{-1}$
$Km_{3PG}$	0.19	mM
$Km_{2PG}$	0.12	mM