Fructose 1,6 bisphosphate aldolase

This enzyme splits fructose 1, 6-bisphosphate into two sugars that are isomers of each other. These two sugars are Dihydroxyacetone phosphate (DHAP) and Glyceraldehyde 3-phosphate (Gly3P).

Chemical equation

$Fru1,6BP \rightleftharpoons Gly3P + DHAP$

Rate equation

Reversible Uni-Bi Michaelis-Menten is used.

$\frac{V_{mf} \frac{[Fru1,6BP]}{K_{Fru1,6BP}} - V_{mr}\frac{[DHAP][G3P]}{K_{DHAP}K_{Gly3P}} }{1 + \frac{[Fru1,6BP]}{K_{Fru1,6BP}} + \frac{[DHAP]}{K_{DHAP}} +\frac{[Gly3P]}{K_{Gly3P}} + \frac{[DHAP][Gly3P]}{K_{DHAP}K_{Gly3P}} }$

Parameters

Parameter	Value	Units	Organism	Remarks
$V_{mf}$	0.08	$mM \times min^{-1}$	Hela cell line
$V_{mr}$	0.063	$mM \times min^{-1}$	Rodent AS-30D hepatoma
$Km_{Fru1,6BP}$	0.009	mM	Hela cell line
$Km_{Gly3P}$	0.16	mM	Rodent AS-30D hepatoma
$Km_{DHAP}$	0.08	mM	Rodent AS-30D hepatoma

Parameters with uncertainty

Parameter	Value	Units	Organism	Remarks
$V_{mf}$	$0.2 \pm 0.05 (5)$	$mM \times min^{-1}$	Hela cell line
$V_{mr}$	0.063	$mM \times min^{-1}$	Rodent AS-30D hepatoma
$Km_{Fru1,6BP}$	0.009	mM	Hela cell line
$Km_{Gly3P}$	0.16	mM	Rodent AS-30D hepatoma
$Km_{DHAP}$	0.08	mM	Rodent AS-30D hepatoma