Transformation of AA to 15-HPETE

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Reaction

R17 AA - HPETE15.jpg

Chemical equation

 AA \rightleftharpoons PGH2

Rate equation

15-LOX Parameters

Michaelis-Menten Constants
Value Units Species Notes Reference
3.70E-03 ± 3.00E-04  mM Human Epithelium [1]
5.00E-03  mM Human Reticulocyte [2]
1.06E-02  mM Human Keratinocyte [3]
1.17E-02 ± 9.00E-04  mM Human Wildtype 15-Lipoxygenase [4]
Enzyme Turnover Numbers
Value Units Species Notes Reference
595.8 ± 16.8  minute^{-1} Human Epithelium [1]
37.2 ± 1.8 per minute Human prostate epithelial 15-lipoxygenase-2 pH 7 [5]
45 ± 1.2 pH 7.5
44.4 ± 2.4 pH 8
34.2 ± 0.6 15°C
45 ± 1.2 22°C
62.4 ± 4.2 30°C
82.8 ± 4.8 37°C
Enzyme Concentrations
Value Units Species Notes Reference
1.60e-05 - 4.00e-05 mM Human Human reticulocyte 15-lipoxygenase-1, Enzymatic assays were performed in 25 mM Hepes buffer (pH 7.5,

25C), with and without product (12-HETE) addition, in the presence of 25 μM of substrate.

[5]
2.00e-05 mM Human Epithelium [1] _

References

Related Reactions