Transformation of AA to 15-HPETE
Contents
Reaction
Chemical equation
Rate equation
15-LOX Parameters
Value | Units | Species | Notes | Reference |
---|---|---|---|---|
3.70E-03 ± 3.00E-04 | Human | Epithelium | [1] | |
5.00E-03 | Human | Reticulocyte | [2] | |
1.06E-02 | Human | Keratinocyte | [3] | |
1.17E-02 ± 9.00E-04 | Human | Wildtype 15-Lipoxygenase | [4] |
Value | Units | Species | Notes | Reference |
---|---|---|---|---|
595.8 ± 16.8 | Human | Epithelium | [1] | |
37.2 ± 1.8 | per minute | Human prostate epithelial 15-lipoxygenase-2 | pH 7 | [5] |
45 ± 1.2 | pH 7.5 | |||
44.4 ± 2.4 | pH 8 | |||
34.2 ± 0.6 | 15°C | |||
45 ± 1.2 | 22°C | |||
62.4 ± 4.2 | 30°C | |||
82.8 ± 4.8 | 37°C |
Value | Units | Species | Notes | Reference |
---|---|---|---|---|
1.60e-05 - 4.00e-05 | mM | Human | Human reticulocyte 15-lipoxygenase-1, Enzymatic assays were performed in 25 mM Hepes buffer (pH 7.5,
25C), with and without product (12-HETE) addition, in the presence of 25 μM of substrate. |
[5] |
2.00e-05 | mM | Human | Epithelium | [1] _ |
References
- ↑ 1.0 1.1 1.2 Jacquot C. "Isotope sensitive branching and kinetic isotope effects in the reaction of deuterated arachidonic acids with human 12- and 15-lipoxygenases. Biochemistry. 2008 Jul 8;47(27):7295-303. doi: 10.1021/bi800308q. Epub 2008 Jun 12.
- ↑ Jacquot C. "Synthesis of 11-Thialinoleic Acid and 14-Thialinoleic Acid, Inhibitors of Soybean and Human Lipoxygenases Org Biomol Chem. 2008 Nov 21; 6(22): 4242–4252.
- ↑ Burrall B. "Enzymatic properties of the 15-lipoxygenase of human cultured keratinocytes. J Invest Dermatol. 1988 Oct;91(4):294-7.
- ↑ Sloane D. L. "Conversion of human 15-lipoxygenase to an efficient 12-lipoxygenase: the side-chain geometry of amino acids 417 and 418 determine positional specificity. Protein Eng. 1995 Mar;8(3):275-82..
- ↑ 5.0 5.1 Jacquot C. "Kinetic and Structural Investigations of the Allosteric Site in Human Epithelial 15-Lipoxygenase-2 Biochemistry, 2009, 48 (36), pp 8721–8730 Cite error: Invalid
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