Dephosphorylation

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Binding Reaction

 \text{phosphorylated-enzyme} \rightleftharpoons \text{unphosphorylated-enzyme}

Kinetic equation

\frac{v_{max} \cdot [\text{phosphorylated-enzyme}] \cdot (1-\frac{[\text{unphosphorylated-enzyme}]}{[\text{phosphorylated-enzyme}] \cdot K_{eq}})}{1 + \frac{[\text{phosphorylated-enzyme}]}{Km_{\text{phosphorylated-enzyme}}} \cdot \frac{[\text{unphosphorylated-enzyme}]}{Km_{\text{unphosphorylated-enzyme}}}}

final Parameter

v_{max} =  1.13  \cdot 10^{2} \pm 3.09 \cdot 10^{2}\ \frac{1}{s}

K_{m} = 3.83 \cdot 10^{-3} \pm 1.40 \cdot 10^{-2}\ \mu M

Parameter

Notes Data
There are three main deophosphatases, phosphoserine phosphatase (EC 3.1.3.3), phosphoprotein phosphatase (EC 3.1.3.16), protein-tyrosine phosphatase (3.1.3.48). The data available in BRENDA is used to calculate the average and standard deviation.
EC 3.1.3.3 -
phosphoserine phosphatase
EC 3.1.3.16 -
phosphoprotein phosphatase
EC 3.1.3.48 -
protein-tyrosine phosphatase
Km [mM]
average all 0.75 7.43 3.31
std 1.29 27.74 12.90
turnover number [1/s]
average all 1.62*102 1.09 * 102 67.8
std 359.51 210.26 256.88