Transformation of PGE2 to 15-Keto-PGE2

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15-hydroxyprostaglandin dehydrogenase, also known as (15-PGDH) metabolises PG into the 15-Keto variant via a ketone reduction. The enzyme is constituently expressed in the skin (Finhelm1982) and within the eicosanoid network it is reported as metabolising PGE2 and PGF2a.

It should be noted that Judson et al found that 15-PGDH expression of the enzyme decreases in response to UVR (Judson2010).


Reaction

Catalysed by 15-PGDH

Chemical equation

 PGE2 \rightleftharpoons 15-Keto-PGE2

Rate equation

15-PGDH Parameters

Michaelis-Menten Constants
Value Units Species Notes Reference
0.008  mM Rat Skin Method:

pH:7.4 Temperature: 37'C Substrate: PGE2 + NAD+

[1]
0.0075  mM Rat Skin Method:

pH:7.4 Temperature: 37'C Substrate: PGE2 + NADP+

[1]
0.024  mM Rat Skin Method:

pH:7.4 Temperature: 37'C Substrate: PGF2a + NAD+

[1]
0.023  mM Rat Skin Method:

pH:7.4 Temperature: 37'C Substrate: PGF2a + NADP+

[1]


Enzyme Turnover Numbers
Value Units Species Notes Reference
 per minute
cPLA2 Abundance
Value Units Species Notes Reference
 ppm
Gibbs Free Energy Change
Value Units Species Notes Reference

References