Mitocondrial pyruvate metabolism
Mitocondrial pyruvate metabolism(MPM) is an enzyme that generates ATP form pyruvate.
Chemical reaction
Rate equation
- Chemical reactions proceed to equilibrium within closed systems. For a simple reaction it is defined as where forward and reverse rates are equal.
- Equilibrium is not reached in open system due to influx and outflux. Mass action ratio[1] for reaction is defined as where subscript ob represents observable at a given point.
- Deviation from equilibrium is measured with Disequilibrium constant as
- Given the simple uni molecular reaction the mass action equation can be modified as
Considering and we have,
- The generalized arbitrary mass action ratio gives us
For eg. for the reaction , the rate law would be
- This equation demonstrates how a rate expression can be divided into parts that include both kinetics and thermodynamic properties [2].
- Given the net rate of reaction , we have
In this model
Parameter values
Parameter | Value | Organism | Remarks |
---|---|---|---|
[4] | HeLa cell line | Constant flux |
References
- ↑ Hess B. and Brand K. (1965), Enzymes and metabolite profiles. In Control of energy metabolism. III. Ed. B. Chance, R. K. Estabrook and J. R. Williamson. New York: Academic Press
- ↑ Sauro H M, Enzyme Kinetics for Systems Biology, Second Edition, Ambrosius Publishing (2013), ISBN-10: 0-9824773-3-3
- ↑ Owusu-Apenten R. Introduction to Food Chemistry, First Edition, CRC Press (2004), ISBN-10: 084931724X
- ↑ 4.0 4.1 Marín-Hernández A, Gallardo-Pérez JC, Rodríguez-Enríquez S et al (2011) Modeling cancer glycolysis. Biochim Biophys Acta 1807:755–767 (doi)