HaCaT Fatty acid analysis Results

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The aim of this study was to estimate the levels of AA in cells which were unstimulated and stimulated with A23187 (with/without COX inhibition), ATP and UVR. GC-FID was used for this fatty acid analysis and the values were normalized using cell numbers (ug /10⁶ cells).

Summary Table

The estimated AA levels in HaCaT keratinocytes (unstimulated, A23187 (5µM), COX inhibited and A23187 (5 µM), ATP (2 mM) and UVR (15 mJ/cm2)) for 0.5, 1, 3 and 6h. Analysis was performed using GC-FID. Results are expressed mean ± SD (ug/106 cells) of three independent experiments.

Time (h)	Experiment
	Unstimulated	A23187	Indomethacin + A23187	UVR	ATP
0	2.87 ± 0.28	2.87 ± 0.28	2.87 ± 0.28	2.87 ± 0.28	2.87 ± 0.28
0.5	2.68 ± 0.49	0.28 ± 0.07	4.18 ± 0.47	2.41 ± 0.70	1.27 ± 0.23
1	2.57 ± 1.25	0.21 ± 0.09	3.42 ± 0.47	2.15 ± 0.72	1.44 ± 0.41
3	2.51 ± 0.82	0.25 ± 0.20	1.98 ± 0.34	1.74 ± 0.77	1.15 ± 0.20
6	2.91 ± 0.22	0.28 ± 0.35	1.13 ± 0.49	0.79 ± 0.82	1.47 ± 0.28

Estimation of cellular arachidonic acid in unstimulated cells

Estimation of AA in unstimulated HaCaT keratinocytes over 6h. The data presented is from three independent experiments.

Estimation of cellular arachidonic acid following stimulation with calcium ionophore (A23187)

Estimation of AA in HaCaT keratinocytes stimulated with A23187 (5 µM) for 6h. The data presented is from three independent experiments.

Estimation of cellular arachidonic acid following stimulation with ATP

Estimation of AA in HaCaT keratinocytes stimulated with ATP (2 mM) for 6h. The data presented is from three independent experiments.

Estimation of cellular arachidonic acid following stimulation with UVR

Estimation of AA in HaCaT keratinocytes over 6h following stimulation with UVR (15 mJ/cm2). The data presented is from three independent experiments.

Estimation of cellular arachidonic acid following inhibition with indomethacin

Estimation of AA in HaCaT keratinocytes inhibited with indomethacin (10 mM, 1h) and stimulated with A23187 (5 µM) for 6h. The data presented is from three independent experiments.