Difference between revisions of "Lactate dehydrogenase"
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==Rate equation== | ==Rate equation== | ||
| + | RAndom Bi-Bi reversible Michaelis-Menten equation is used. <ref name="Hernandez2011"> Marín-Hernández A, Gallardo-Pérez JC, Rodríguez-Enríquez S et al (2011) Modeling cancer glycolysis. Biochim Biophys Acta 1807:755–767 ([http://dx.doi.org/10.1016/j.bbabio.2010.11.006 doi]) </ref> | ||
<center><math> \frac{V_{mf}\frac{[NADH][PYR]}{K_{NADH} K_{PYR}} - V_{mr}\frac{[Lactate_{in}][NAD]}{K_{Lactate_{in}} K_{NAD}}}{1 + \frac{[NADH]}{K_{NADH}} + \frac{[PYR]}{K_{PYR}} + \frac{[NADH][PYR]}{K_{NADH} K_{PYR}} + \frac{[Lactate_{in}][NAD]}{K_{Lactate_{in}} K_{NAD}} + \frac{[Lactate_{in}]}{K_{Lactate_{in}}} + \frac{[PYR]}{K_{PYR}} } </math></center> | <center><math> \frac{V_{mf}\frac{[NADH][PYR]}{K_{NADH} K_{PYR}} - V_{mr}\frac{[Lactate_{in}][NAD]}{K_{Lactate_{in}} K_{NAD}}}{1 + \frac{[NADH]}{K_{NADH}} + \frac{[PYR]}{K_{PYR}} + \frac{[NADH][PYR]}{K_{NADH} K_{PYR}} + \frac{[Lactate_{in}][NAD]}{K_{Lactate_{in}} K_{NAD}} + \frac{[Lactate_{in}]}{K_{Lactate_{in}}} + \frac{[PYR]}{K_{PYR}} } </math></center> | ||
Revision as of 18:23, 4 March 2014
A dehydrogenase is an enzyme that transfers a hydride from one molecule to another. Lactate dehydrogenase catalyzes the conversion of pyruvate to lactate and back, as it converts NADH to NAD+ and back.
Chemical reactions
Rate equation
RAndom Bi-Bi reversible Michaelis-Menten equation is used. [1]
![\frac{V_{mf}\frac{[NADH][PYR]}{K_{NADH} K_{PYR}} - V_{mr}\frac{[Lactate_{in}][NAD]}{K_{Lactate_{in}} K_{NAD}}}{1 + \frac{[NADH]}{K_{NADH}} + \frac{[PYR]}{K_{PYR}} + \frac{[NADH][PYR]}{K_{NADH} K_{PYR}} + \frac{[Lactate_{in}][NAD]}{K_{Lactate_{in}} K_{NAD}} + \frac{[Lactate_{in}]}{K_{Lactate_{in}}} + \frac{[PYR]}{K_{PYR}} }](/wiki/images/math/e/b/0/eb0bc9b0022002ad69cab019a0bd568a.png)
Prameter values
| Parameter | Value | Organism | Remarks |
|---|---|---|---|
|
11.4 [1] | HeLa cell line | |
|
1.8[1] | Rat AS-30D hepatoma | |
|
0.1[1] | HeLa cell line | |
|
4.7[1] | Rat AS-30D hepatoma | |
|
0.07[1] | HeLa cell line | |
|
0.002[1] | HeLa cell line |
