Difference between revisions of "Transformation of 5-HPETE to 5-HETE"

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Revision as of 10:58, 12 August 2016

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R12 HPETE5-HETE5.jpg

Chemical equation

AA \rightleftharpoons PGH2

Rate equation

PHGPx Parameters

Michaelis-Menten Constants
Value Units Species Notes Reference
3.00E-01 mM Human Expression Vector: Bioimprited Enzyme - Selenosubtilisin

Enzyme: Glutathione Peroxidase pH: 7 Temperature: 37

[1]
11.1 ± 2.90E-01 mM Human Expression Vector: E Coli

Enzyme: Wild Type Glutathione Peroxidase Enzyme (Se-hGSTZ1-1) pH: 7 Temperature: 37

[2]
4.00E-03 mM Rat Expression Vector: Rat Liver Cells

Enzyme: Glutathione Peroxidase pH: 7.4 Temperature: 37

[3]
Enzyme Turnover Numbers
Value Units Species Notes Reference
1170 ± 50 min^{-1} Human Expression Vector: Bioimprited Enzyme - Selenosubtilisin

Enzyme: Glutathione Peroxidase pH: 7 Temperature: 37

[1]
24500 ± 150 min^{-1} Human Expression Vector: E Coli

Enzyme: Wild Type Glutathione Peroxidase Enzyme (Se-hGSTZ1-1) pH: 7 Temperature: 37

[2]
PHGPx Abundance
Value Units Species Notes Reference
459 ppm Human Expression Vector: Skin

Enzyme: PHGPx pH: 7.5 Temperature: 37 °C

[4]
307 ppm Human Expression Vector: Lung

Enzyme: PHGPx pH: 7.5 Temperature: 37 °C

[4]
282 ppm Human Expression Vector: Esophagus

Enzyme: PHGPx pH: 7.5 Temperature: 37 °C

[4]
204 ppm Human Expression Vector: Gut

Enzyme: PHGPx pH: 7.5 Temperature: 37 °C

</ref>[5]

References

  1. ↑ 1.0 1.1 Liu L. "Functional mimicry of the active site of glutathione peroxidase by glutathione imprinted selenium-containing protein. Biomacromolecules. 2008 Jan;9(1):363-8. doi: 10.1021/bm7008312. Epub 2007 Dec 29.
  2. ↑ 2.0 2.1 Zheng K. "A novel selenium-containing glutathione transferase zeta1-1, the activity of which surpasses the level of some native glutathione peroxidases. Int J Biochem Cell Biol. 2008;40(10):2090-7. doi: 10.1016/j.biocel.2008.02.006. Epub 2008 Feb 15.
  3. ↑ Hiratsuka A. "Subunit Ya-specific glutathione peroxidase activity toward cholesterol 7-hydroperoxides of glutathione S-transferases in cytosols from rat liver and skin. J Biol Chem. 1997 Feb 21;272(8):4763-9.
  4. ↑ 4.0 4.1 4.2 M. Wilhelm Mass-spectrometry-based draft of the human proteome Nature, 2014 509, 582–587
  5. ↑ M. Kim A draft map of the human proteome Nature, 2014 509, 575–581

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