Difference between revisions of "Transformation of AA to 12-HPETE"

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(12-LOX Parameters)
(12-LOX Parameters)
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! Reference
 
! Reference
 
|-
 
|-
|97.3
+
|19.8
 
|<math> ppm </math>
 
|<math> ppm </math>
 
|Human  
 
|Human  
|Expression Vector: Lung
+
|Expression Vector: Spleen
Enzyme: 5-LOX
+
Enzyme: 12-LOX
 +
pH: 7.5
 +
Temperature: 37 °C
 +
|<ref name="Wilhelm2014"> [http://www.nature.com/nature/journal/v509/n7502/pdf/nature13319.pdf M. Wilhelm ''Mass-spectrometry-based draft of the human proteome'' Nature, 2014 509, 582–587]</ref>
 +
|-
 +
|1.60
 +
|<math> ppm </math>
 +
|Human
 +
|Expression Vector: Liver
 +
Enzyme: 12-LOX
 
pH: 7.5
 
pH: 7.5
 
Temperature: 37 °C
 
Temperature: 37 °C
 
|<ref name="Kim2014"> [http://www.nature.com/nature/journal/v509/n7502/pdf/nature13302.pdf M. Kim ''A draft map of the human proteome'' Nature, 2014 509, 575–581]</ref>
 
|<ref name="Kim2014"> [http://www.nature.com/nature/journal/v509/n7502/pdf/nature13302.pdf M. Kim ''A draft map of the human proteome'' Nature, 2014 509, 575–581]</ref>
 
|-
 
|-
|49.8
+
|0.28
 
|<math> ppm </math>
 
|<math> ppm </math>
 
|Human  
 
|Human  
|Expression Vector: Esophagus
+
|Expression Vector: Gut
Enzyme: 5-LOX
+
Enzyme: 12-LOX
 
pH: 7.5
 
pH: 7.5
 
Temperature: 37 °C
 
Temperature: 37 °C
|<ref name="Wilhelm2014"> [http://www.nature.com/nature/journal/v509/n7502/pdf/nature13319.pdf M. Wilhelm ''Mass-spectrometry-based draft of the human proteome'' Nature, 2014 509, 582–587]</ref>
+
|<ref name="Kim2014"> [http://www.nature.com/nature/journal/v509/n7502/pdf/nature13302.pdf M. Kim ''A draft map of the human proteome'' Nature, 2014 509, 575–581]</ref>
 
|-
 
|-
|31.9
+
|0.11
 
|<math> ppm </math>
 
|<math> ppm </math>
 
|Human  
 
|Human  
|Expression Vector: Oral Cavity
+
|Expression Vector: Pancreas
Enzyme: 5-LOX
+
Enzyme: 12-LOX
 
pH: 7.5
 
pH: 7.5
 
Temperature: 37 °C
 
Temperature: 37 °C
|<ref name="Wilhelm2014"> [http://www.nature.com/nature/journal/v509/n7502/pdf/nature13319.pdf M. Wilhelm ''Mass-spectrometry-based draft of the human proteome'' Nature, 2014 509, 582–587]</ref>
+
|<ref name="Kim2014"> [http://www.nature.com/nature/journal/v509/n7502/pdf/nature13302.pdf M. Kim ''A draft map of the human proteome'' Nature, 2014 509, 575–581]</ref>
 
|-
 
|-
 
|}
 
|}

Revision as of 10:48, 12 August 2016

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R179AA - HPETE12.jpg

Chemical equation

 AA \rightleftharpoons PGH2

Rate equation

12-LOX Parameters

Michaelis-Menten Constants
Value Units Species Notes Reference
7.20E-03  mM Human Expression Vector: Platelet

Enzyme: 12-Lipoxygenase pH: 7.4 Temperature: 37°C.

[1]
8.00E-02  mM Human Expression Vector: Platelet

Enzyme: 12-Lipoxygenase pH: 7 Temperature: 24

[2]
1.00E-02  mM Human Platlet Expression Vector: Baculovirus

Enzyme: 12-Lipoxygenase pH: 8 Temperature: 37

[3]
7.90E-03 ± 8.00E-04  mM Human Expression Vector: Platelets

Enzyme:12-Lipoxygenase pH: 7.4 Temperature: 37

[4]
Enzyme Turnover Numbers
Value Units Species Notes Reference
336 ± 12 per minute Human Expression Vector: Human reticulocyte

Enzyme: 15-lipoxygenase-1 pH: 7.5 Temperature: 25

[5]
504 per minute Wild Boar Expression Vector: E. coli.

Enzyme: 12-Lipoxygenase pH: 7.4 Temperature: 37

[6]


12-LOX Abundance
Value Units Species Notes Reference
19.8  ppm Human Expression Vector: Spleen

Enzyme: 12-LOX pH: 7.5 Temperature: 37 °C

[7]
1.60  ppm Human Expression Vector: Liver

Enzyme: 12-LOX pH: 7.5 Temperature: 37 °C

[8]
0.28  ppm Human Expression Vector: Gut

Enzyme: 12-LOX pH: 7.5 Temperature: 37 °C

[8]
0.11  ppm Human Expression Vector: Pancreas

Enzyme: 12-LOX pH: 7.5 Temperature: 37 °C

[8]

References

Related Reactions