Difference between revisions of "Transformation of AA to 12-HPETE"

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(12-LOX Parameters)
(12-LOX Parameters)
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{|class="wikitable sortable"  
 
{|class="wikitable sortable"  
|+  style="text-align: left;" | Enzyme Concentrations
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|+  style="text-align: left;" | 12-LOX Abundance
 
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! Value
 
! Value
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! Reference
 
! Reference
 
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|5.00e-06 - 6.00e-05
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|97.3
|mM
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|<math> ppm </math>
|Human
+
|Human  
|Expression Vector: Human reticulocyte
+
|Expression Vector: Lung
Enzyme: 15-lipoxygenase-1
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Enzyme: 5-LOX
 
pH: 7.5
 
pH: 7.5
Temperature: 25
+
Temperature: 37 °C
|<ref name="Wecksler2009"> [www.ncbi.nlm.nih.gov/pubmed/19469483 Wecksler A. "Mechanistic Investigations of Human Reticulocyte 15- and Platelet 12-Lipoxygenases with Arachidonic Acid'' Biochemistry, 2009, 48 (26), pp 6259–6267]</ref>
+
|<ref name="Kim2014"> [http://www.nature.com/nature/journal/v509/n7502/pdf/nature13302.pdf M. Kim ''A draft map of the human proteome'' Nature, 2014 509, 575–581]</ref>
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|49.8
 +
|<math> ppm </math>
 +
|Human
 +
|Expression Vector: Esophagus
 +
Enzyme: 5-LOX
 +
pH: 7.5
 +
Temperature: 37 °C
 +
|<ref name="Wilhelm2014"> [http://www.nature.com/nature/journal/v509/n7502/pdf/nature13319.pdf M. Wilhelm ''Mass-spectrometry-based draft of the human proteome'' Nature, 2014 509, 582–587]</ref>
 +
|-
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|31.9
 +
|<math> ppm </math>
 +
|Human  
 +
|Expression Vector: Oral Cavity
 +
Enzyme: 5-LOX
 +
pH: 7.5
 +
Temperature: 37 °C
 +
|<ref name="Wilhelm2014"> [http://www.nature.com/nature/journal/v509/n7502/pdf/nature13319.pdf M. Wilhelm ''Mass-spectrometry-based draft of the human proteome'' Nature, 2014 509, 582–587]</ref>
 
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Revision as of 09:47, 10 August 2016

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R179AA - HPETE12.jpg

Chemical equation

 AA \rightleftharpoons PGH2

Rate equation

12-LOX Parameters

Michaelis-Menten Constants
Value Units Species Notes Reference
7.20E-03  mM Human Expression Vector: Platelet

Enzyme: 12-Lipoxygenase pH: 7.4 Temperature: 37°C.

[1]
8.00E-02  mM Human Expression Vector: Platelet

Enzyme: 12-Lipoxygenase pH: 7 Temperature: 24

[2]
1.00E-02  mM Human Platlet Expression Vector: Baculovirus

Enzyme: 12-Lipoxygenase pH: 8 Temperature: 37

[3]
7.90E-03 ± 8.00E-04  mM Human Expression Vector: Platelets

Enzyme:12-Lipoxygenase pH: 7.4 Temperature: 37

[4]
Enzyme Turnover Numbers
Value Units Species Notes Reference
336 ± 12 per minute Human Expression Vector: Human reticulocyte

Enzyme: 15-lipoxygenase-1 pH: 7.5 Temperature: 25

[5]
504 per minute Wild Boar Expression Vector: E. coli.

Enzyme: 12-Lipoxygenase pH: 7.4 Temperature: 37

[6]


12-LOX Abundance
Value Units Species Notes Reference
97.3  ppm Human Expression Vector: Lung

Enzyme: 5-LOX pH: 7.5 Temperature: 37 °C

[7]
49.8  ppm Human Expression Vector: Esophagus

Enzyme: 5-LOX pH: 7.5 Temperature: 37 °C

[8]
31.9  ppm Human Expression Vector: Oral Cavity

Enzyme: 5-LOX pH: 7.5 Temperature: 37 °C

[8]

References

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