Difference between revisions of "Transformation of AA to 12-HPETE"
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− | |+ style="text-align: left;" | | + | |+ style="text-align: left;" | 12-LOX Abundance |
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! Value | ! Value | ||
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! Reference | ! Reference | ||
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− | | | + | |97.3 |
− | | | + | |<math> ppm </math> |
− | |Human | + | |Human |
− | |Expression Vector: | + | |Expression Vector: Lung |
− | Enzyme: | + | Enzyme: 5-LOX |
pH: 7.5 | pH: 7.5 | ||
− | Temperature: | + | Temperature: 37 °C |
− | |<ref name=" | + | |<ref name="Kim2014"> [http://www.nature.com/nature/journal/v509/n7502/pdf/nature13302.pdf M. Kim ''A draft map of the human proteome'' Nature, 2014 509, 575–581]</ref> |
+ | |- | ||
+ | |49.8 | ||
+ | |<math> ppm </math> | ||
+ | |Human | ||
+ | |Expression Vector: Esophagus | ||
+ | Enzyme: 5-LOX | ||
+ | pH: 7.5 | ||
+ | Temperature: 37 °C | ||
+ | |<ref name="Wilhelm2014"> [http://www.nature.com/nature/journal/v509/n7502/pdf/nature13319.pdf M. Wilhelm ''Mass-spectrometry-based draft of the human proteome'' Nature, 2014 509, 582–587]</ref> | ||
+ | |- | ||
+ | |31.9 | ||
+ | |<math> ppm </math> | ||
+ | |Human | ||
+ | |Expression Vector: Oral Cavity | ||
+ | Enzyme: 5-LOX | ||
+ | pH: 7.5 | ||
+ | Temperature: 37 °C | ||
+ | |<ref name="Wilhelm2014"> [http://www.nature.com/nature/journal/v509/n7502/pdf/nature13319.pdf M. Wilhelm ''Mass-spectrometry-based draft of the human proteome'' Nature, 2014 509, 582–587]</ref> | ||
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Revision as of 09:47, 10 August 2016
Contents
Reaction
Chemical equation
Rate equation
12-LOX Parameters
Value | Units | Species | Notes | Reference |
---|---|---|---|---|
7.20E-03 | Human | Expression Vector: Platelet
Enzyme: 12-Lipoxygenase pH: 7.4 Temperature: 37°C. |
[1] | |
8.00E-02 | Human | Expression Vector: Platelet
Enzyme: 12-Lipoxygenase pH: 7 Temperature: 24 |
[2] | |
1.00E-02 | Human Platlet | Expression Vector: Baculovirus
Enzyme: 12-Lipoxygenase pH: 8 Temperature: 37 |
[3] | |
7.90E-03 ± 8.00E-04 | Human | Expression Vector: Platelets
Enzyme:12-Lipoxygenase pH: 7.4 Temperature: 37 |
[4] |
Value | Units | Species | Notes | Reference |
---|---|---|---|---|
336 ± 12 | per minute | Human | Expression Vector: Human reticulocyte
Enzyme: 15-lipoxygenase-1 pH: 7.5 Temperature: 25 |
[5] |
504 | per minute | Wild Boar | Expression Vector: E. coli.
Enzyme: 12-Lipoxygenase pH: 7.4 Temperature: 37 |
[6] |
Value | Units | Species | Notes | Reference |
---|---|---|---|---|
97.3 | Human | Expression Vector: Lung
Enzyme: 5-LOX pH: 7.5 Temperature: 37 °C |
[7] | |
49.8 | Human | Expression Vector: Esophagus
Enzyme: 5-LOX pH: 7.5 Temperature: 37 °C |
[8] | |
31.9 | Human | Expression Vector: Oral Cavity
Enzyme: 5-LOX pH: 7.5 Temperature: 37 °C |
[8] |
References
- ↑ Lagarde M. "Subcellular localization and some properties of lipoxygenase activity in human blood platelets. Biochem J. 1984 Sep 1;222(2):495-500.
- ↑ Hada T. "Catalytic properties of human platelet 12-lipoxygenase as compared with the enzymes of other origins. Biochim Biophys Acta. 1991 Apr 24;1083(1):89-93.
- ↑ Chen X. S. "Purification and characterization of recombinant histidine-tagged human platelet 12-lipoxygenase expressed in a baculovirus/insect cell system. Eur J Biochem. 1993 Jun 15;214(3):845-52.
- ↑ Romano M. "Lipoxin synthase activity of human platelet 12-lipoxygenase. Biochem J. 1993 Nov 15;296 ( Pt 1):127-33.
- ↑ [www.ncbi.nlm.nih.gov/pubmed/19469483 Wecksler A. "Mechanistic Investigations of Human Reticulocyte 15- and Platelet 12-Lipoxygenases with Arachidonic Acid Biochemistry, 2009, 48 (26), pp 6259–6267]
- ↑ Richards K. "Leukocyte 12-Lipoxygenase: Expression, Purification, and Investigation of the Role of Methionine Residues in Turnover-Dependent Inactivation and 5,8,11,14-Eicosatetraynoic Acid Inhibition Biochemistry, 1997, 36 (22), pp 6692–6699
- ↑ M. Kim A draft map of the human proteome Nature, 2014 509, 575–581
- ↑ 8.0 8.1 M. Wilhelm Mass-spectrometry-based draft of the human proteome Nature, 2014 509, 582–587