Difference between revisions of "Hexose-6-phosphate isomerase"

Revision as of 12:42, 4 March 2014

The enzyme Hexose-6-phosphate isomerase converts Glucose 6-phosphate (Glc6P) into its isomer Fructose 6-phosphate (Fru6P). Isomers have the same molecular formula, but the atoms of each molecule are arranged differently.

Glc6P \rightleftharpoons Fru6P

Reversible competitive inhibition with Ery4P, 6PG and FBP. ^[1]

\frac{V_{mf}\frac{[Glc6P]}{Km_{Glc6P}} - V_{mr} \frac{[Fru6P]}{Km_{Fru6P}} }{ 1 + \frac{[Glc6P]}{Km_{Glc6P}} + \frac{[Fru6P]}{Km_{Fru6P}} + \frac{[ERY4P]}{Km_{ERY4P}} + \frac{[6PG]}{Km_{6PG}} + \frac{[FBP]}{Km_{FBP}} }

Parameter	Value	Organism	Remarks
$V_{mf}$	1.2 $\pm$ 0.2 mM ^[1]	HeLa cell line	3 cell assays
$V_{mr}$	2.8^[1]		1 cell assay
$Km_{Glc6P}$	0.4 $\pm$ 0.03 mM^[1]		3 cell assays
$Km_{Fru6P}$	0.05^[1]		2 cell assays
$Km_{Glc6P}$	$2e^{-002}$ ^[1]
$Km_{ADP}$	3.5^[1]

↑ ^1.0 ^1.1 ^1.2 ^1.3 ^1.4 ^1.5 ^1.6 Marín-Hernández A, Gallardo-Pérez JC, Rodríguez-Enríquez S et al (2011) Modeling cancer glycolysis. Biochim Biophys Acta 1807:755–767 (doi) Cite error: Invalid <ref> tag; name "Hernandez2011" defined multiple times with different content Cite error: Invalid <ref> tag; name "Hernandez2011" defined multiple times with different content

@@ Line 8: / Line 8: @@
 Reversible competitive inhibition with Ery4P, 6PG and FBP. <ref name="Hernandez2011"> Marín-Hernández A, Gallardo-Pérez JC, Rodríguez-Enríquez S et al (2011) Modeling cancer glycolysis. Biochim Biophys Acta 1807:755–767 ([http://dx.doi.org/10.1016/j.bbabio.2010.11.006 doi])  </ref>
-<center><math>\frac{V_{mf}\frac{[Glc6P]}{K_{Glc6P}} - V_{mr} \frac{[Fru6P]}{K_{Fru6P}} }{ 1 + \frac{[Glc6P]}{K_{Glc6P}} + \frac{[Fru6P]}{K_{Fru6P}} + \frac{[ERY4P]}{K_{ERY4P}} + \frac{[6PG]}{K_{6PG}} + \frac{[FBP]}{K_{FBP}} }</math></center>
+<center><math>\frac{V_{mf}\frac{[Glc6P]}{Km_{Glc6P}} - V_{mr} \frac{[Fru6P]}{Km_{Fru6P}} }{ 1 + \frac{[Glc6P]}{Km_{Glc6P}} + \frac{[Fru6P]}{Km_{Fru6P}} + \frac{[ERY4P]}{Km_{ERY4P}} + \frac{[6PG]}{Km_{6PG}} + \frac{[FBP]}{Km_{FBP}} }</math></center>
 ==Parameters==