Difference between revisions of "Transformation of AA to 5-HPETE"

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(5-LOX Parameters)
Line 6: Line 6:
 
==Chemical equation==
 
==Chemical equation==
  
<center><math> AA \rightleftharpoons PGH2 </math></center>
+
<center><math> PGH2 \rightleftharpoons 5-HPETE </math></center>
  
 
== Rate equation ==
 
== Rate equation ==

Revision as of 13:57, 8 June 2016

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R11 AA - HPETE5.jpg

Chemical equation

 PGH2 \rightleftharpoons 5-HPETE

Rate equation

5-LOX Parameters

Michaelis-Menten Constants
Value Units Species Notes Reference
5.10E-03  mM Human Expression Vector: Baculovirus, Sf9 insect cells

Enzyme: Recombinant 5-Lipoxygenase pH: 5.6 Temperature:37

[1]
1.20E-02  mM Human Expression Vector: Polymorphonuclear Leukocytes

Enzyme: 5-Lipoxygenase pH:7.5 Temperature: 22

[2]
6.31E-02  mM Human Expression Vector: Polymorphonuclear Leukocytes

Enzyme: 5-Lipoxygenase pH:7.5 Temperature: 22

[3]
Enzyme Turnover Numbers
Value Units Species Notes Reference
1500 + 75 per minute Potato Expression Vector:Potato Tubers

Enzyme: 5-Lipoxygenase pH:5.5 Temperature: 23

[4]
Michaelis-Menten Constants
Value Units Species Notes Reference
5.19e-03 mM Human Calculated by 0.4mg of 77kDa of protein in 1 mL.

Leukocytes

[5]

References


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