Difference between revisions of "Transformation of AA to 5-HPETE"
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+ | |+ style="text-align: left;" | Michaelis-Menten Constants | ||
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+ | ! Value | ||
+ | ! Units | ||
+ | ! Species | ||
+ | ! Notes | ||
+ | ! Reference | ||
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+ | |5.19e-03 | ||
+ | |mM | ||
+ | |Human | ||
+ | |Calculated by 0.4mg of 77kDa of protein in 1 mL. | ||
+ | Leukocytes | ||
+ | |<ref name="Rouzer1990”>[http://ac.els-cdn.com/000629529190110Q/1-s2.0-000629529190110Q-main.pdf?_tid=8b9511da-0c72-11e6-b92e 00000aab0f26&acdnat=1461759858_b7fd721a8b568158e0c0a4843425c1e4 Rouzer C., “Inhibition of human leukocyte 5-lipoxygenase by a 4-hydroxybenzofuran, L-656,224: Evidence for enzyme reduction and inhibitor degradation” Biochemical Pharmacology, Volume 41, Issue 9, 1 May 1991, Pages 1365-1373]</ref> | ||
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Revision as of 12:32, 27 April 2016
Contents
Reaction
Chemical equation
Rate equation
5-LOX Parameters
Value | Units | Species | Notes | Reference |
---|---|---|---|---|
5.10E-03 | Human | Recombinant 5-Lipoxygenase | [1] | |
1.20E-02 | Human | PMN Leukocyte | [2] | |
6.31E-02 | Human | Polymorphonuclear Leukocytes | [3] |
Value | Units | Species | Notes | Reference |
---|---|---|---|---|
1500 + 75 | per minute | Potato | Potato Tubers, pH 5.5, | [4] |
Value | Units | Species | Notes | Reference |
---|---|---|---|---|
5.19e-03 | mM | Human | Calculated by 0.4mg of 77kDa of protein in 1 mL.
Leukocytes |
[5] |
References
- ↑ Shirumalla R. K. “RBx 7,796: A novel inhibitor of 5-lipoxygenase.” Inflamm Res. 2006 Dec ; 55 (12) : 517-27.
- ↑ Soberman R. J. "5- and 15(omega-6)-lipoxygenases from human polymorphonuclear leukocytes. Methods Enzymol. 1988; 163:344-9.
- ↑ Soberman R. J. “Characterization and separation of the arachidonic acid 5-lipoxygenase and linoleic acid omega-6 lipoxygenase (arachidonic acid 15-lipoxygenase) of human polymorphonuclear leukocytes.” J Biol Chem. 1985 Apr 10;260(7):4508-15.
- ↑ Mulliez E., “5-Lipoxygenase from potato tubers. Improved purification and physicochemical characteristics” Biochimica et Biophysica Acta, 1987;916(1):13-23.
- ↑ 00000aab0f26&acdnat=1461759858_b7fd721a8b568158e0c0a4843425c1e4 Rouzer C., “Inhibition of human leukocyte 5-lipoxygenase by a 4-hydroxybenzofuran, L-656,224: Evidence for enzyme reduction and inhibitor degradation” Biochemical Pharmacology, Volume 41, Issue 9, 1 May 1991, Pages 1365-1373