Difference between revisions of "Hexokinase"
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|<math>Km_{ADP}</math> | |<math>Km_{ADP}</math> | ||
|3.5<ref name="Hernandez2011"></ref> | |3.5<ref name="Hernandez2011"></ref> | ||
| + | |} | ||
| + | |||
| + | |||
| + | ====With inhibition==== | ||
| + | {|class="wikitable" | ||
| + | ! Parameter | ||
| + | ! Value | ||
| + | ! Organism | ||
| + | ! Remarks | ||
| + | |- | ||
| + | |<math>V_{mf}</math> | ||
| + | |0.06 <ref name="Hernandez_2006"> Marín-Hernández A , Rodríguez-Enríquez S, Vital-González P A, ''et al.'' (2006). ''Determining and understanding the control of glycolysis in fast-growth tumor cells. Flux control by an over-expressed but strongly product-inhibited hexokinase''. FEBS J., 273 , pp. 1975–1988([http://dx.doi.org/doi:10.1111/j.1742-4658.2006.05214.x doi]) </ref> | ||
| + | |rowspan="6"|HeLa cell line | ||
| + | |rowspan="6"| | ||
| + | |- | ||
| + | |<math>Km_{Glucose}</math> | ||
| + | |0.1<ref name="Hernandez2011"></ref> | ||
| + | |- | ||
| + | |<math>Km_{ATP}</math> | ||
| + | |1.1<ref name="Hernandez2011"></ref> | ||
| + | |- | ||
| + | |<math>Ki_{Glc6P}</math> | ||
| + | |<math>0.02</math><ref name="Wilson_2003">Wilson J E (2003). ''Isozymes of mammalian hexokinase: structure, subcellular localization and metabolic function''. J. Exp. Biology, 206, pp. 2049–2057</ref> | ||
|} | |} | ||
Revision as of 12:14, 4 March 2014
The enzyme hexokinase phosphorylates (adds a phosphate group to) glucose in the cell's cytoplasm. In the process, a phosphate group from Adenosine triphosphate (ATP) is transferred to glucose producing glucose 6-phosphate (Glc6P).
Contents
Chemical equation
Rate equation
Without inhibition
Random Bi-Bi Michaelis Menten is used.[1]
![v = \frac{\frac{V_{mf}}{Km_{Glucose_{in}}K_{ATP}}\Big( [Glucose_{in}][ATP] - \frac{[Glc6P][ADP]}{K_{eq}} \Big)}{1 + \frac{[Glucose_{in}]}{Km_{Glucose_{in}}} + \frac{[ATP]}{K_{ATP}} + \frac{[Glucose_{in}][ATP]}{Km_{Glucose_{in}}K_{ATP}} + \frac{[Glc6P]}{K_{Glc6P}} + \frac{[ADP]}{K_{ADP}} +\frac{[Glc6P][ADP]}{K_{Glc6P}K_{ADP}} + \frac{[Glucose_{in}][ADP]}{K_{Glucose_{in}}K_{ADP}} +\frac{[Glc6P][ATP]}{K_{Glc6P}K_{ATP}} }](/wiki/images/math/d/2/d/d2d1c3d1115eef8cdf23e7eb98f65b04.png)
With allosteric inhibition
![\frac{V_{mf}* \frac{[Glucose]*[ATP]}{Km_{Glucose}*Km_{ATP}} }{ \left(1 + \frac{[Glc6P]}{Ki_{Glc6P}} \right)* \left( 1 + \frac{[Glucose]}{Km_{Glucose}} \right) + \frac{[ATP]}{Km_{ATP}} + \frac{[Glucose]*[ATP]}{Km_{Glucose}*Km_{ATP}} }](/wiki/images/math/c/2/6/c26962b0cefb2c26a75d64c2de18328e.png)
Parameters
Without inhibition
| Parameter | Value | Organism | Remarks |
|---|---|---|---|
|
0.06 [2] | HeLa cell line | |
|
651[3] | ||
|
0.1[1] | ||
|
1.1[1] | ||
|
 [1]
| ||
|
3.5[1] |
With inhibition
| Parameter | Value | Organism | Remarks |
|---|---|---|---|
|
|
0.06 [2] | HeLa cell line | |
|
|
0.1[1] | ||
|
|
1.1[1] | ||
|
 [4]
|
Alternative parameter values
References
- ↑ 1.0 1.1 1.2 1.3 1.4 1.5 1.6 Marín-Hernández A, Gallardo-Pérez JC, Rodríguez-Enríquez S et al (2011) Modeling cancer glycolysis. Biochim Biophys Acta 1807:755–767 (doi)
- ↑ 2.0 2.1 Marín-Hernández A , Rodríguez-Enríquez S, Vital-González P A, et al. (2006). Determining and understanding the control of glycolysis in fast-growth tumor cells. Flux control by an over-expressed but strongly product-inhibited hexokinase. FEBS J., 273 , pp. 1975–1988(doi)
- ↑ Arbitrary value
- ↑ Wilson J E (2003). Isozymes of mammalian hexokinase: structure, subcellular localization and metabolic function. J. Exp. Biology, 206, pp. 2049–2057
