Difference between revisions of "Limonene Synthase: Detailed information on the kinetic values obtained from the literature"
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! Description | ! Description | ||
! References | ! References | ||
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+ | |kcat<sub>forward</sub> | ||
+ | | 0.09 | ||
+ | | s<sup>-1</sup> | ||
+ | | CsTPS1 (limonene producing terpene synthase), Km of 6.25 ± 0.41 mM, pH 6.5, 40°C, [GPP] 0.5 - 25.0 mM used for kinetic parameter determination, kcat 0.09 <sup>-1</sup> | ||
+ | [[file: 35_LimSynth_Km_Kcat_Gunnewich08.png |400px | An excerpt from Gunnewich 2008 Thesis ]] | ||
+ | | <ref name="Gunnewich2008"> </ref> | ||
|- | |- | ||
| kcat<sub>forward</sub> | | kcat<sub>forward</sub> |
Revision as of 15:18, 15 April 2016
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Contents
Kinetic Parameters
Turnover numbers (Kcat)
Parameter | Value | Units | Description | References |
---|---|---|---|---|
kcatforward | 0.09 | s-1 | CsTPS1 (limonene producing terpene synthase), Km of 6.25 ± 0.41 mM, pH 6.5, 40°C, [GPP] 0.5 - 25.0 mM used for kinetic parameter determination, kcat 0.09 -1 | [1] |
kcatforward | 3.9x10-5 | s-1 | Limonene synthase expressed at 37°C, 40µM GPP, Citrus sinensis, product formation 1.17x105 µM/sec
|
[2] |
kcatforward | 0.13 | s-1 | Limonene synthase expressed at 20°C, 200µM GPP, Citrus sinensis, pH 7.5 | [3] |
kcatforward | 0.04 | s-1 | Limonene synthase expressed at 37°C, 200µM GPP, Citrus sinensis, pH 7.5 | [3] |
Kcat | 0.3 | s-1 | Limonene synthase assay- Mentha species, pH7.0, 30°C, typical reaction mixture contained 1-10 μg protein and 15mM MgCl2, Initial [GPP} is 10μM | [4] |
Kcat | 0.020 | s-1 | [5] | |
Kcat | 0.024 | s-1 | Below is an excerpt from Williams 1998, but data source is from Ogura 1997, describing data from wildtype E.coli | [6] |
Kcat | 0.082 | s-1 | Kcat value for CsTPS1, Cannabis sativa, pH6.5, 40°C, enzyme assay | [7] |
Michaelis-Menten constant (Km)
Parameter | Value | Units | Description | References |
---|---|---|---|---|
KmGPP | 6.25 ± 0.41 | µM | CsTPS1 (limonene producing), Km of 6.25 ± 0.41 mM, pH 6.5, 40°C, [GPP] 0.5 - 25.0 mM used for kinetic parameter determination, kcat 0.09 s-1 | [1] |
KmGPP | 130 | µM | [3] | |
Km | 0.7 | µM | [8] | |
KmGPP | 1.25 | µM | [9] | |
KmGPP | 6.8 | µM | Km value for CsTPS1, pH6.5, 40°C, Cannabis sativa enzyme assay | [7] |
KmGPP | 8.6 | µM | Km value for E.coli Q53, from Williams 1998 | [5] |
KmGPP | 6.0 | µM | Km value for E.coli E57, from Williams 1998 | [5] |
KmGPP | 12.6 | µM | Km value for E.coli R58, from Williams 1998 | [5] |
KmGPP | 29.9 | µM | Km value for E.coli R59, from Williams 1998 | [5] |
KmGPP | 16 | µM | [5] | |
KmGPP | 6.7 | µM | Data from Ogura 1997, from wildtype E.coli, as described in Williams 1998. ( I will get the book!) | [6] |
Substrate and Enzyme Concentrations or Amount
Parameter | Value | Units | Description | References |
---|---|---|---|---|
[GPP] | 40 | µM | Limonene synthase expressed at 37°C, 40µM GPP, Citrus sinensis, product formation 1.17x105 µM/sec | [2] |
[GPP] | 10 | µM | Limonene synthase expressed at 37°C, 40µM GPP, Citrus sinensis, product formation 1.17x105 µM/sec, [E]: 0.3 µM
|
[2] |
[LimSynth] | 0.3 | µM | Limonene synthase expressed at 37°C, 10µM GPP, Citrus sinensis, product formation 1.17x105 µM/sec, [E]: 0.3 µM | [2] |
[LimSynth] | 0.07 | µM | Limonene synthase expressed at 20°C, 200µM GPP, Citrus sinensis | [3] |
[LimSynth] | 0.26 | µM | Limonene synthase expressed at 37°C, 200µM GPP, Citrus sinensis, pH 7.5 | [3] |
Enzyme amount | 1-10 | µg | [4] | |
Enzyme amount | 10-20 | µg | [9] | |
[GPP] | 10 | µM | [4] | |
[GPP] | 2 | µM | [8] | |
[GPP] | 0.1 | µM | 0.1 -100 µM | [8] |
[GPP] | 100 | µM | 0.1 -100 µM | [8] |
[LimSynth] | 1 - 50 | µg | Amount of native or recombinant protein (E.coli) used for Limonene synthase enzyme assay | [5] |
[GPP] | 1 - 40 | µM | Substrate concentration range for enzyme assay | [5] |
[GPP] | 10 | µM | [7] | |
Enzyme Amount (Possibly) | 1.25 | µg | Possible it is referring to enzyme amount used for CsTPS1 (limonene producing terpene synthase).
|
[7] |
[GPP] | 25 | µM | GPP concentration used, for preprotein (?) expressed in E.coli. | [5] |
Enzyme amount | 150 | µg | Purified (-)-4S-limonene synthase amount. | [5] |
Maximum velocity (Vmax), Enzyme activity & Specific enzyme activity
Parameter | Value | Units | Description | References |
---|---|---|---|---|
Vmaxforward | 0.53 | µM/min | Limonene synthase expressed at 20°C, 200µM GPP, Citrus sinensis, pH7.5 | [3] |
Vmaxforward | 0.64 | µM/min | Limonene synthase expressed at 37°C, 200µM GPP | [3] |
Specific enzyme activity | 19 | µmol/h/mg | [4] |
Other information
Parameter | Value | Units | Description | References |
---|---|---|---|---|
Limonene Synthase
Molecular Weight |
56 kDa | kDa | Limonene synthase purification | [4] |
References
- ↑ 1.0 1.1 Cite error: Invalid
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tag; name "Gunnewich2008" defined multiple times with different content - ↑ 2.0 2.1 2.2 2.3 Olsen, S. N. 2011. "Isolation, Purification, and Characterization of (+)-4R-limonene synthase: The first step in exploring enzyme stereospecificity in terpenoid biosynthesis", Masters Thesis, Brandeis Univeristy
- ↑ 3.0 3.1 3.2 3.3 3.4 3.5 3.6 Entova, S. 2013. "Kinetic characterization, crystallization, and photosynthetic expression of (+)-4R-limonene synthase from C. sinensis", Masters Thesis, Brandeis Univeristy
- ↑ 4.0 4.1 4.2 4.3 4.4 Alonso et. al. 1992. "Purification of 4S-Limonene Synthase, a Monoterpene Cyclase from the Glandular Trichomes of Peppermint (Mentha x piperita) and Spearmint (Mentha spicata)", The journal of Biological Chemistry, 267(11):7582-7587
- ↑ 5.0 5.1 5.2 5.3 5.4 5.5 5.6 5.7 5.8 5.9 Williams, D.C. et. al. 1998. [www.ncbi.nlm.nih.gov/pubmed/9724535 "Truncation of Limonene Synthase preprotein provides a fully active 'pseudomature' form of this monoterpene cyclase and reveals the function of the amino-terminal arginine pair ",Biochemistry, 37:12213-12220] Cite error: Invalid
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tag; name "Williams1998" defined multiple times with different content Cite error: Invalid<ref>
tag; name "Williams1998" defined multiple times with different content Cite error: Invalid<ref>
tag; name "Williams1998" defined multiple times with different content Cite error: Invalid<ref>
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tag; name "Williams1998" defined multiple times with different content Cite error: Invalid<ref>
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tag; name "Williams1998" defined multiple times with different content - ↑ 6.0 6.1 Ogura, K. & Koyama, T. 1997. "Dynamic Aspects of Natural Products Chemistry", pp 1-23 Cite error: Invalid
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tag; name "Ogura1997" defined multiple times with different content - ↑ 7.0 7.1 7.2 7.3 Cite error: Invalid
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tag; name "Gunnewich2006" defined multiple times with different content Cite error: Invalid<ref>
tag; name "Gunnewich2006" defined multiple times with different content - ↑ 8.0 8.1 8.2 8.3 Cite error: Invalid
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tag; name "Lucker2002" defined multiple times with different content - ↑ 9.0 9.1 Adam, K. et. al. 1996. "Partial purification and characterization of a monoterpene cyclase, limonene synthase, from the liverwort Ricciocarpos natans. 332(2):pp 352-356.