Difference between revisions of "Hexokinase"
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== Rate equation == | == Rate equation == | ||
− | ===Without inhibition=== | + | ====Without inhibition==== |
Random Bi-Bi Michaelis Menten is used.<ref name="Hernandez2011"> Marín-Hernández A, Gallardo-Pérez JC, Rodríguez-Enríquez S et al (2011) Modeling cancer glycolysis. Biochim Biophys Acta 1807:755–767 ([http://dx.doi.org/10.1016/j.bbabio.2010.11.006 doi]) </ref> | Random Bi-Bi Michaelis Menten is used.<ref name="Hernandez2011"> Marín-Hernández A, Gallardo-Pérez JC, Rodríguez-Enríquez S et al (2011) Modeling cancer glycolysis. Biochim Biophys Acta 1807:755–767 ([http://dx.doi.org/10.1016/j.bbabio.2010.11.006 doi]) </ref> | ||
<center><math> v = \frac{\frac{V_{mf}}{Km_{Glucose_{in}}K_{ATP}}\Big( [Glucose_{in}][ATP] - \frac{[Glc6P][ADP]}{K_{eq}} \Big)}{1 + \frac{[Glucose_{in}]}{Km_{Glucose_{in}}} + \frac{[ATP]}{K_{ATP}} + \frac{[Glucose_{in}][ATP]}{Km_{Glucose_{in}}K_{ATP}} + \frac{[Glc6P]}{K_{Glc6P}} + \frac{[ADP]}{K_{ADP}} +\frac{[Glc6P][ADP]}{K_{Glc6P}K_{ADP}} + \frac{[Glucose_{in}][ADP]}{K_{Glucose_{in}}K_{ADP}} +\frac{[Glc6P][ATP]}{K_{Glc6P}K_{ATP}} } </math></center> | <center><math> v = \frac{\frac{V_{mf}}{Km_{Glucose_{in}}K_{ATP}}\Big( [Glucose_{in}][ATP] - \frac{[Glc6P][ADP]}{K_{eq}} \Big)}{1 + \frac{[Glucose_{in}]}{Km_{Glucose_{in}}} + \frac{[ATP]}{K_{ATP}} + \frac{[Glucose_{in}][ATP]}{Km_{Glucose_{in}}K_{ATP}} + \frac{[Glc6P]}{K_{Glc6P}} + \frac{[ADP]}{K_{ADP}} +\frac{[Glc6P][ADP]}{K_{Glc6P}K_{ADP}} + \frac{[Glucose_{in}][ADP]}{K_{Glucose_{in}}K_{ADP}} +\frac{[Glc6P][ATP]}{K_{Glc6P}K_{ATP}} } </math></center> | ||
− | ===With allosteric inhibition=== | + | ====With allosteric inhibition==== |
<center><math> \frac{V_{mf}* \frac{[Glucose]*[ATP]}{Km_{Glucose}*Km_{ATP}} }{ \left(1 + \frac{[Glc6P]}{Ki_{Glc6P}} \right)* \left( 1 + \frac{[Glucose]}{Km_{Glucose}} \right) + \frac{[ATP]}{Km_{ATP}} + \frac{[Glucose]*[ATP]}{Km_{Glucose}*Km_{ATP}} } </math></center> | <center><math> \frac{V_{mf}* \frac{[Glucose]*[ATP]}{Km_{Glucose}*Km_{ATP}} }{ \left(1 + \frac{[Glc6P]}{Ki_{Glc6P}} \right)* \left( 1 + \frac{[Glucose]}{Km_{Glucose}} \right) + \frac{[ATP]}{Km_{ATP}} + \frac{[Glucose]*[ATP]}{Km_{Glucose}*Km_{ATP}} } </math></center> | ||
Revision as of 11:48, 4 March 2014
The enzyme hexokinase phosphorylates (adds a phosphate group to) glucose in the cell's cytoplasm. In the process, a phosphate group from Adenosine triphosphate (ATP) is transferred to glucose producing glucose 6-phosphate (Glc6P).
Contents
Chemical equation
Rate equation
Without inhibition
Random Bi-Bi Michaelis Menten is used.[1]
With allosteric inhibition
Parameters
Parameter | Value | Organism | Remarks |
---|---|---|---|
0.46 [2] | HeLa cell line | Vm in U × (mg total cellular protein) at C | |
1[3] | |||
9.3 | |||
10[3] |
Alternative parameter values
References
- ↑ Marín-Hernández A, Gallardo-Pérez JC, Rodríguez-Enríquez S et al (2011) Modeling cancer glycolysis. Biochim Biophys Acta 1807:755–767 (doi)
- ↑ Marín-Hernández A , Rodríguez-Enríquez S, Vital-González P A, et al. (2006). Determining and understanding the control of glycolysis in fast-growth tumor cells. Flux control by an over-expressed but strongly product-inhibited hexokinase. FEBS J., 273 , pp. 1975–1988(doi)
- ↑ 3.0 3.1 Arbitrary value