Difference between revisions of "Geranyl diphosphate synthase: Detailed information on the kinetic values obtained from the literature"

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(→‎Detailed description of the values used for the GPPS model)
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| 390
 
| 390
 
| µM
 
| µM
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| [[file:22_GPPS_Km_Vmax_Schmidt10.png  |400px | An excerpt from Schmidt, et. al. 2010]]
+
| [[file:22_GPPS_Km_Vmax_Schmidt10.png  |300px | An excerpt from Schmidt, et. al. 2010]]
 
| <ref name="Schmidt2010">Schmidt, A. 2010. [http://www.ncbi.nlm.nih.gov/pubmed/19939949 "A bifunctional geranyl and geranylgeranyl diphosphate synthase is involved in terpene oleoresin formation in ''Picea abies''", Plant Physiology, 152:639-655]</ref>
 
| <ref name="Schmidt2010">Schmidt, A. 2010. [http://www.ncbi.nlm.nih.gov/pubmed/19939949 "A bifunctional geranyl and geranylgeranyl diphosphate synthase is involved in terpene oleoresin formation in ''Picea abies''", Plant Physiology, 152:639-655]</ref>
 +
|-
 +
| Km<sub>IPP</sub>
 +
| 170
 +
| µM
 +
| [[file:22_GPPS_Km_Vmax_Schmidt10.png  |300px | An excerpt from Schmidt, et. al. 2010]]
 +
| <ref name="Schmidt2010"> </ref>
 
|-
 
|-
 
| Km<sub>DMAPP</sub>
 
| Km<sub>DMAPP</sub>

Revision as of 14:41, 29 March 2016

Back to terpene model MAIN page.

Back to the GPPS model page.


Detailed description of the values used for the GPPS model

Parameter Value Units Description References
KmDMAPP 390 µM An excerpt from Schmidt, et. al. 2010 [1]
KmIPP 170 µM An excerpt from Schmidt, et. al. 2010 [1]
KmDMAPP 58 µM An excerpt from Mann, et. al. 2011 [2]
KmIPP 163 µM An excerpt from Mann, et. al. 2011 [2]
Kcat 0.7 min-1 An excerpt from Mann, et. al. 2011 [2]
Kcat 5.1 min-1 An excerpt from Mann, et. al. 2011 [2]
KmDMAPP 11.60 µM with Co2+ as a cofactor, and IPP as a co-substrate

An excerpt from Frick et al, 2013, Table 1, page 4198

[3]
KmIPP 0.84 µM with Co2+ as a cofactor, and DMAPP as co-substrate

An excerpt from Frick et al, 2013, Table 1, page 4198

[3]
KmDMAPP 1103 µM with Mg2+ as a cofactor, and IPP as co-substrate

An excerpt from Frick et al, 2013, Table 1, page 4198

[3]
KmIPP 11.79 µM with Mg2+ as a cofactor, and DMAPP as co-substrate

An excerpt from Frick et al, 2013, Table 1, page 4198

[3]
[IPP] 15 µM with Co2+ as a cofactor

An excerpt from Frick et al, 2013, Table 1, page 4198

[3]
[DMAPP] 50 µM with Co2+ as a cofactor

An excerpt from Frick et al, 2013, Table 1, page 4198

[3]
Vmaxforward 0.44 µmol min-1mg-1 with Co2+ as a cofactor

An excerpt from Frick et al, 2013, Table 1, page 4198

[3]
Vmaxforward 0.67 µmol min-1mg-1 with Co2+ as a cofactor

An excerpt from Frick et al, 2013, Table 1, page 4198

[3]
Vmaxforward 2.39 µmol min-1mg-1 with Mg2+ as a cofactor

An excerpt from Frick et al, 2013, Table 1, page 4198

[3]
Vmaxforward 0.17 µmol min-1mg-1 with Mg2+ as a cofactor

An excerpt from Frick et al, 2013, Table 1, page 4198

[3]
[IPP] 50 µM with Mg2+ as a cofactor

An excerpt from Frick et al, 2013, Table 1, page 4198

[3]
[DMAPP] 50 µM with Mg2+ as a cofactor

An excerpt from Frick et al, 2013, Table 1, page 4198

[3]

Reference

  1. ↑ 1.0 1.1 Schmidt, A. 2010. "A bifunctional geranyl and geranylgeranyl diphosphate synthase is involved in terpene oleoresin formation in Picea abies", Plant Physiology, 152:639-655 Cite error: Invalid <ref> tag; name "Schmidt2010" defined multiple times with different content
  2. ↑ 2.0 2.1 2.2 2.3 Mann, F.M. 2011. "Rv0989c encodes a novel (E)-geranyl diphosphate synthase facilitating decaprenyl diphosphate biosynthesis in Mycobacterium tuberculosis", FEBS Letters, 585(3):549-554 Cite error: Invalid <ref> tag; name "Mann2011" defined multiple times with different content Cite error: Invalid <ref> tag; name "Mann2011" defined multiple times with different content Cite error: Invalid <ref> tag; name "Mann2011" defined multiple times with different content
  3. ↑ 3.00 3.01 3.02 3.03 3.04 3.05 3.06 3.07 3.08 3.09 3.10 3.11 Frick, S. 2013. "Metal ions control product specificity of isoprenyl diphosphate synthases in the insect terpenoid pathway", PNAS, 110(11):4194-4199
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