Difference between revisions of "Limonene Synthase: Detailed information on the kinetic values obtained from the literature"
Aliah.hawari (talk | contribs) |
Aliah.hawari (talk | contribs) |
||
| Line 16: | Line 16: | ||
| [[file: 02 LimSynth Enz GPP Alonso92.png |600px | An excerpt from Alonso 1992, Materials and Methods section]] | | [[file: 02 LimSynth Enz GPP Alonso92.png |600px | An excerpt from Alonso 1992, Materials and Methods section]] | ||
| <ref name="Alonso1992">Alonso et. al. 1992. [http://www.ncbi.nlm.nih.gov/pubmed/1559995 "Purification of 4S-Limonene Synthase, a Monoterpene Cyclase from the Glandular Trichomes of Peppermint (Mentha x piperita) and Spearmint (Mentha spicata)", The journal of Biological Chemistry, 267(11):7582-7587]</ref> | | <ref name="Alonso1992">Alonso et. al. 1992. [http://www.ncbi.nlm.nih.gov/pubmed/1559995 "Purification of 4S-Limonene Synthase, a Monoterpene Cyclase from the Glandular Trichomes of Peppermint (Mentha x piperita) and Spearmint (Mentha spicata)", The journal of Biological Chemistry, 267(11):7582-7587]</ref> | ||
| + | |- | ||
| + | | Enzyme amount | ||
| + | | 10-20 | ||
| + | | µg | ||
| + | | [[file: 19_LimSynth_Enz_act_Adam1996.png |600px | An excerpt from Adam 1996, Materials and Methods section]] | ||
| + | | <ref name="Adam1996"> Adam, K. et. al. 1996. "Partial purification and characterization of a monoterpene cyclase, limonene synthase, from the liverwort ''Ricciocarpos natans''. 332(2):pp 352-356.</ref> | ||
|- | |- | ||
| [GPP] | | [GPP] | ||
Revision as of 17:31, 21 March 2016
Back to terpene model MAIN page.
| Parameter | Value | Units | Description | References |
|---|---|---|---|---|
| Enzyme amount | 1-10 | µg | 
|
[1] |
| Enzyme amount | 10-20 | µg | 
|
[2] |
| [GPP] | 10 | µM |
|
[1] |
| [GPP] | 2 | µM | 
|
[3] |
| KmGPP | 1.25 | µM | 
|
[2] |
| Specific enzyme activity | 19 | µmol/h/mg | 
|
[1] |
| Kcat | 0.3 | s-1 |
|
[1] |
| Kcat | 0.020 | s-1 | 
|
[4] |
| Kcat | 0.024 | s-1 | Below is an excerpt from Williams 1998, but data source is from Ogura 1997, describing data from wildtype E.coli
|
[5] |
| [GPP] | 10 | µM | 
|
[6] |
| Enzyme Amount (Possibly) | 1.25 | µg | Possible it is referring to enzyme amount used for CsTPS1 (limonene producing terpene synthase).
|
[6] |
| KmGPP | 6.8 | µM | Km and Kcat value for CsTPS1 (limonene producing terpene synthase)
|
[6] |
| KmGPP | 8.6 | µM | Km value for E.coli Q53, from Williams 1998
|
[4] |
| KmGPP | 6.0 | µM | Km value for E.coli E57, from Williams 1998
|
[4] |
| KmGPP | 12.6 | µM | Km value for E.coli R58, from Williams 1998
|
[4] |
| KmGPP | 29.9 | µM | Km value for E.coli R59, from Williams 1998
|
[4] |
| KmGPP | 16 | µM |
|
[4] |
| KmGPP | 6.7 | µM | Data from Ogura 1997, from wildtype E.coli, as described in Williams 1998. ( I will get the book!)
|
[5] |
| Kcat | 0.082 | s-1 | Km and Kcat value for CsTPS1 (limonene producing terpene synthase)
|
[6] |
| [GPP] | 25 | µM | GPP concentration used, for preprotein (?) expressed in E.coli.
|
[4] |
| Enzyme amount | 150 | µg | Purified (-)-4S-limonene synthase amount.
|
[4] |
References
- ↑ 1.0 1.1 1.2 1.3 Alonso et. al. 1992. "Purification of 4S-Limonene Synthase, a Monoterpene Cyclase from the Glandular Trichomes of Peppermint (Mentha x piperita) and Spearmint (Mentha spicata)", The journal of Biological Chemistry, 267(11):7582-7587
- ↑ 2.0 2.1 Adam, K. et. al. 1996. "Partial purification and characterization of a monoterpene cyclase, limonene synthase, from the liverwort Ricciocarpos natans. 332(2):pp 352-356.
- ↑ Lücker, J. et. al. 2002."Monoterpene biosynthesis in lemon (Citrus limon). cDNA isolation and functional analysis of four monoterpene synthases", Eur. J. Biochem. 269: pp3160-3171
- ↑ 4.0 4.1 4.2 4.3 4.4 4.5 4.6 4.7 Williams, D.C. et. al. 1998. [www.ncbi.nlm.nih.gov/pubmed/9724535 "Truncation of Limonene Synthase preprotein provides a fully active 'pseudomature' form of this monoterpene cyclase and reveals the function of the amino-terminal arginine pair ",Biochemistry, 37:12213-12220] Cite error: Invalid
<ref>tag; name "Williams1998" defined multiple times with different content Cite error: Invalid<ref>tag; name "Williams1998" defined multiple times with different content Cite error: Invalid<ref>tag; name "Williams1998" defined multiple times with different content Cite error: Invalid<ref>tag; name "Williams1998" defined multiple times with different content Cite error: Invalid<ref>tag; name "Williams1998" defined multiple times with different content Cite error: Invalid<ref>tag; name "Williams1998" defined multiple times with different content Cite error: Invalid<ref>tag; name "Williams1998" defined multiple times with different content - ↑ 5.0 5.1 Ogura, K. & Koyama, T. 1997. "Dynamic Aspects of Natural Products Chemistry", pp 1-23 Cite error: Invalid
<ref>tag; name "Ogura1997" defined multiple times with different content - ↑ 6.0 6.1 6.2 6.3 Günnewich, N. et. al. 2006. "Functional expression and characterization of trichome-specific (-)-limonene synthase and (+)-α-pinene synthase from Cannabis sativa", Natural Product Communications, 0(0):1-10 Cite error: Invalid
<ref>tag; name "Gunnewich2006" defined multiple times with different content Cite error: Invalid<ref>tag; name "Gunnewich2006" defined multiple times with different content Cite error: Invalid<ref>tag; name "Gunnewich2006" defined multiple times with different content
