Difference between revisions of "Limonene Synthase: Detailed information on the kinetic values obtained from the literature"
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| [[file: 15_LimSynth_Km_Kcat_Williams98.png| 450px | An excerpt from Williams 1998, Result section]] | | [[file: 15_LimSynth_Km_Kcat_Williams98.png| 450px | An excerpt from Williams 1998, Result section]] | ||
| − | | <ref name="Williams1998"></ref> | + | | <ref name="Williams1998"> <ref name="Williams1998"> Williams, D.C. et. al. 1998. [www.ncbi.nlm.nih.gov/pubmed/9724535 "Truncation of Limonene Synthase preprotein provides a fully active 'pseudomature' form of this monoterpene cyclase and reveals the function of the amino-terminal arginine pair ",Biochemistry, 37:12213-12220]</ref> |
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| Kcat | | Kcat | ||
Revision as of 17:55, 18 March 2016
Back to terpene model MAIN page.
| Parameter | Value | Units | Description | References |
|---|---|---|---|---|
| Enzyme amount | 1-10 | µg | 
|
[1] |
| [GPP] | 10 | µM |
|
[1] |
| Specific enzyme activity | 19 | µmol/h/mg | 
|
[1] |
| Kcat | 0.3 | s-1 |
|
[1] |
| Kcat | 0.020 | s-1 | 
|
Cite error: Closing </ref> missing for <ref> tag
|
| Kcat | 0.024 | s-1 | Below is an excerpt from Williams 1998, but data source is from Ogura 1997, describing data from wildtype E.coli
|
[2] |
| [GPP] | 10 | µM | 
|
[3] |
| Enzyme Amount (Possibly) | 1.25 | µg | Possible it is referring to enzyme amount used for CsTPS1 (limonene producing terpene synthase).
|
[3] |
| KmGPP | 6.8 | µM | Km and Kcat value for CsTPS1 (limonene producing terpene synthase)
|
[3] |
| KmGPP | 8.6 | µM | Km value for E.coli Q53, from Williams 1998
|
[4] |
| KmGPP | 6.0 | µM | Km value for E.coli E57, from Williams 1998
|
[4] |
| KmGPP | 12.6 | µM | Km value for E.coli R58, from Williams 1998
|
[4] |
| KmGPP | 29.9 | µM | Km value for E.coli R59, from Williams 1998
|
[4] |
| KmGPP | 16 | µM |
|
[4] |
| KmGPP | 6.7 | µM | Data from Ogura 1997, from wildtype E.coli, as described in Williams 1998. ( I will get the book!)
|
[2] |
| Kcat | 0.082 | s-1 | Km and Kcat value for CsTPS1 (limonene producing terpene synthase)
|
[3] |
| [GPP] | 25 | µM | GPP concentration used, for preprotein (?) expressed in E.coli.
|
[4] |
| Enzyme amount | 150 | µg | Purified (-)-4S-limonene synthase amount.
|
[4] |
References
- ↑ 1.0 1.1 1.2 1.3 Alonso et. al. 1992. "Purification of 4S-Limonene Synthase, a Monoterpene Cyclase from the Glandular Trichomes of Peppermint (Mentha x piperita) and Spearmint (Mentha spicata)", The journal of Biological Chemistry, 267(11):7582-7587
- ↑ 2.0 2.1 Ogura, K. & Koyama, T. 1997. "Dynamic Aspects of Natural Products Chemistry", pp 1-23 Cite error: Invalid
<ref>tag; name "Ogura1997" defined multiple times with different content - ↑ 3.0 3.1 3.2 3.3 Günnewich, N. et. al. 2006. "Functional expression and characterization of trichome-specific (-)-limonene synthase and (+)-α-pinene synthase from Cannabis sativa", Natural Product Communications, 0(0):1-10 Cite error: Invalid
<ref>tag; name "Gunnewich2006" defined multiple times with different content Cite error: Invalid<ref>tag; name "Gunnewich2006" defined multiple times with different content Cite error: Invalid<ref>tag; name "Gunnewich2006" defined multiple times with different content - ↑ 4.0 4.1 4.2 4.3 4.4 4.5 4.6 Cite error: Invalid
<ref>tag; name "Williams1998" defined multiple times with different content Cite error: Invalid<ref>tag; name "Williams1998" defined multiple times with different content
