Difference between revisions of "Limonene Synthase: Detailed information on the kinetic values obtained from the literature"
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[[file: 12_LimSynth_Km_Kcat_Gunnewich06.png | 300px | An excerpt from Günnewich 2006]] | [[file: 12_LimSynth_Km_Kcat_Gunnewich06.png | 300px | An excerpt from Günnewich 2006]] | ||
| <ref name="Gunnewich2006"> </ref> | | <ref name="Gunnewich2006"> </ref> | ||
+ | |- | ||
+ | | Km<sub>GPP</sub> | ||
+ | | 8.6 | ||
+ | | µM | ||
+ | | Km value for E.coli ''Q53'', from Williams 1998 | ||
+ | [[file: 16_LimSynth_EColi_Km_Kcat_Williams98.png | 300px | An excerpt from Günnewich 2006]] | ||
+ | | <ref name="Williams1998"> </ref> | ||
+ | |- | ||
+ | | Km<sub>GPP</sub> | ||
+ | | 6.0 | ||
+ | | µM | ||
+ | | Km value for E.coli ''E57'', from Williams 1998 | ||
+ | [[file: 16_LimSynth_EColi_Km_Kcat_Williams98.png | 300px | An excerpt from Günnewich 2006]] | ||
+ | | <ref name="Williams1998"> </ref> | ||
+ | |- | ||
+ | | Km<sub>GPP</sub> | ||
+ | | 12.6 | ||
+ | | µM | ||
+ | | Km value for E.coli ''R58'', from Williams 1998 | ||
+ | [[file: 16_LimSynth_EColi_Km_Kcat_Williams98.png | 300px | An excerpt from Günnewich 2006]] | ||
+ | | <ref name="Williams1998"> </ref> | ||
+ | |- | ||
+ | | Km<sub>GPP</sub> | ||
+ | | 29.9 | ||
+ | | µM | ||
+ | | Km value for E.coli ''R59'', from Williams 1998 | ||
+ | [[file: 16_LimSynth_EColi_Km_Kcat_Williams98.png | 300px | An excerpt from Günnewich 2006]] | ||
+ | | <ref name="Williams1998"> </ref> | ||
|- | |- | ||
| Km<sub>GPP</sub> | | Km<sub>GPP</sub> |
Revision as of 17:54, 18 March 2016
Back to terpene model MAIN page.
Parameter | Value | Units | Description | References |
---|---|---|---|---|
Enzyme amount | 1-10 | µg | [1] | |
[GPP] | 10 | µM | [1] | |
Specific enzyme activity | 19 | µmol/h/mg | [1] | |
Kcat | 0.3 | s-1 | [1] | |
Kcat | 0.020 | s-1 | [2] | |
Kcat | 0.024 | s-1 | Below is an excerpt from Williams 1998, but data source is from Ogura 1997, describing data from wildtype E.coli | [3] |
[GPP] | 10 | µM | [4] | |
Enzyme Amount (Possibly) | 1.25 | µg | Possible it is referring to enzyme amount used for CsTPS1 (limonene producing terpene synthase).
|
[4] |
KmGPP | 6.8 | µM | Km and Kcat value for CsTPS1 (limonene producing terpene synthase) | [4] |
KmGPP | 8.6 | µM | Km value for E.coli Q53, from Williams 1998 | [2] |
KmGPP | 6.0 | µM | Km value for E.coli E57, from Williams 1998 | [2] |
KmGPP | 12.6 | µM | Km value for E.coli R58, from Williams 1998 | [2] |
KmGPP | 29.9 | µM | Km value for E.coli R59, from Williams 1998 | [2] |
KmGPP | 16 | µM | [2] | |
KmGPP | 6.7 | µM | Data from Ogura 1997, from wildtype E.coli, as described in Williams 1998. ( I will get the book!) | [3] |
Kcat | 0.082 | s-1 | Km and Kcat value for CsTPS1 (limonene producing terpene synthase) | [4] |
[GPP] | 25 | µM | GPP concentration used, for preprotein (?) expressed in E.coli. | [2] |
Enzyme amount | 150 | µg | Purified (-)-4S-limonene synthase amount. | [2] |
References
- ↑ 1.0 1.1 1.2 1.3 Alonso et. al. 1992. "Purification of 4S-Limonene Synthase, a Monoterpene Cyclase from the Glandular Trichomes of Peppermint (Mentha x piperita) and Spearmint (Mentha spicata)", The journal of Biological Chemistry, 267(11):7582-7587
- ↑ 2.0 2.1 2.2 2.3 2.4 2.5 2.6 2.7 Cite error: Invalid
<ref>
tag; name "Williams1998" defined multiple times with different content Cite error: Invalid<ref>
tag; name "Williams1998" defined multiple times with different content - ↑ 3.0 3.1 Ogura, K. & Koyama, T. 1997. "Dynamic Aspects of Natural Products Chemistry", pp 1-23 Cite error: Invalid
<ref>
tag; name "Ogura1997" defined multiple times with different content - ↑ 4.0 4.1 4.2 4.3 Günnewich, N. et. al. 2006. "Functional expression and characterization of trichome-specific (-)-limonene synthase and (+)-α-pinene synthase from Cannabis sativa", Natural Product Communications, 0(0):1-10 Cite error: Invalid
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tag; name "Gunnewich2006" defined multiple times with different content Cite error: Invalid<ref>
tag; name "Gunnewich2006" defined multiple times with different content Cite error: Invalid<ref>
tag; name "Gunnewich2006" defined multiple times with different content