Difference between revisions of "Limonene Synthase: Detailed information on the kinetic values obtained from the literature"
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| GPP concentration used, for preprotein (?) expressed in E.coli. | | GPP concentration used, for preprotein (?) expressed in E.coli. | ||
[[file: 13_LimSynth_GPP_conc_Williams98.png | 400px | An excerpt from Williams 1998, Materials and Methods section]] | [[file: 13_LimSynth_GPP_conc_Williams98.png | 400px | An excerpt from Williams 1998, Materials and Methods section]] | ||
| − | | <ref name="Williams1998"> Williams, D.C. et. al. 1998. [www. | + | | <ref name="Williams1998"> Williams, D.C. et. al. 1998. [https://www.researchgate.net/publication/13560205_Truncation_of_Limonene_Synthase_Preprotein_Provides_a_Fully_Active_'Pseudomature'_Form_of_This_Monoterpene_Cyclase_and_Reveals_the_Function_of_the_Amino-Terminal_Arginine_Pair "Truncation of Limonene Synthase preprotein provides a fully active 'pseudomature' form of this monoterpene cyclase and reveals the function of the amino-terminal arginine pair ",Biochemistry, 37:12213-12220] </ref> |
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| Enzyme amount | | Enzyme amount | ||
Revision as of 17:03, 18 March 2016
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| Parameter | Value | Units | Description | References |
|---|---|---|---|---|
| Enzyme amount | 1-10 | µg | 
|
[1] |
| [GPP] | 10 | µM |
|
[1] |
| Specific enzyme activity | 19 | µmol/h/mg | 
|
[1] |
| Kcat | 0.3 | s-1 |
|
[1] |
| [GPP] | 10 | µM | 
|
[2] |
| Enzyme Amount (Possibly) | 1.25 | µg | Possible it is referring to enzyme amount used for CsTPS1 (limonene producing terpene synthase).
|
[2] |
| KmGPP | 6.8 | µM | Km and Kcat value for CsTPS1 (limonene producing terpene synthase)
|
[2] |
| Kcat | 0.082 | s-1 | Km and Kcat value for CsTPS1 (limonene producing terpene synthase)
|
[2] |
| [GPP] | 25 | µM | GPP concentration used, for preprotein (?) expressed in E.coli.
|
[3] |
| Enzyme amount | 150 | µg | Purified (-)-4S-limonene synthase amount.
|
[3] |
References
- ↑ 1.0 1.1 1.2 1.3 Alonso et. al. 1992. "Purification of 4S-Limonene Synthase, a Monoterpene Cyclase from the Glandular Trichomes of Peppermint (Mentha x piperita) and Spearmint (Mentha spicata)", The journal of Biological Chemistry, 267(11):7582-7587
- ↑ 2.0 2.1 2.2 2.3 Günnewich, N. et. al. 2006. "Functional expression and characterization of trichome-specific (-)-limonene synthase and (+)-α-pinene synthase from Cannabis sativa", Natural Product Communications, 0(0):1-10 Cite error: Invalid
<ref>tag; name "Gunnewich2006" defined multiple times with different content Cite error: Invalid<ref>tag; name "Gunnewich2006" defined multiple times with different content Cite error: Invalid<ref>tag; name "Gunnewich2006" defined multiple times with different content - ↑ 3.0 3.1 Williams, D.C. et. al. 1998. "Truncation of Limonene Synthase preprotein provides a fully active 'pseudomature' form of this monoterpene cyclase and reveals the function of the amino-terminal arginine pair ",Biochemistry, 37:12213-12220 Cite error: Invalid
<ref>tag; name "Williams1998" defined multiple times with different content
