Difference between revisions of "Limonene Synthase: Detailed information on the kinetic values obtained from the literature"
Aliah.hawari (talk | contribs) |
Aliah.hawari (talk | contribs) |
||
Line 61: | Line 61: | ||
[[file: 12_LimSynth_Km_Kcat_Gunnewich06.png | 300px | An excerpt from Günnewich 2006]] | [[file: 12_LimSynth_Km_Kcat_Gunnewich06.png | 300px | An excerpt from Günnewich 2006]] | ||
| <ref name="Gunnewich2006"> </ref> | | <ref name="Gunnewich2006"> </ref> | ||
+ | |- | ||
+ | | [GPP] | ||
+ | | 25 | ||
+ | | µM | ||
+ | | GPP concentration used, for preprotein (?) expressed in E.coli. | ||
+ | [[file: 13_LimSynth_GPP_conc_Williams98.png | 400px | An excerpt from Williams 1998, Materials and Methods section]] | ||
+ | | <ref name="Williams1998"> Williams, D.C. et. al. 1998. [www.ncbi.nlm.nih.gov/pubmed/9724535 "Truncation of Limonene Synthase preprotein provides a fully active 'pseudomature' form of this monoterpene cyclase and reveals the function of the amino-terminal arginine pair ",Biochemistry, 37:12213-12220] </ref> | ||
|- | |- | ||
|} | |} |
Revision as of 16:21, 18 March 2016
Back to terpene model MAIN page.
Parameter | Value | Units | Description | References |
---|---|---|---|---|
Enzyme amount | 1-10 | µg | [1] | |
[GPP] | 10 | µM | [1] | |
Specific enzyme activity | 19 | µmol/h/mg | [1] | |
Kcat | 0.3 | s-1 | [1] | |
[GPP] | 10 | µM | [2] | |
Enzyme Amount (Possibly) | 1.25 | µg | Possible it is referring to enzyme amount used for CsTPS1 (limonene producing terpene synthase).
|
[2] |
KmGPP | 6.8 | µM | Km and Kcat value for CsTPS1 (limonene producing terpene synthase) | [2] |
Kcat | 0.082 | s-1 | Km and Kcat value for CsTPS1 (limonene producing terpene synthase) | [2] |
[GPP] | 25 | µM | GPP concentration used, for preprotein (?) expressed in E.coli. | [3] |
References
- ↑ 1.0 1.1 1.2 1.3 Alonso et. al. 1992. "Purification of 4S-Limonene Synthase, a Monoterpene Cyclase from the Glandular Trichomes of Peppermint (Mentha x piperita) and Spearmint (Mentha spicata)", The journal of Biological Chemistry, 267(11):7582-7587
- ↑ 2.0 2.1 2.2 2.3 Günnewich, N. et. al. 2006. "Functional expression and characterization of trichome-specific (-)-limonene synthase and (+)-α-pinene synthase from Cannabis sativa", Natural Product Communications, 0(0):1-10 Cite error: Invalid
<ref>
tag; name "Gunnewich2006" defined multiple times with different content Cite error: Invalid<ref>
tag; name "Gunnewich2006" defined multiple times with different content Cite error: Invalid<ref>
tag; name "Gunnewich2006" defined multiple times with different content - ↑ Williams, D.C. et. al. 1998. [www.ncbi.nlm.nih.gov/pubmed/9724535 "Truncation of Limonene Synthase preprotein provides a fully active 'pseudomature' form of this monoterpene cyclase and reveals the function of the amino-terminal arginine pair ",Biochemistry, 37:12213-12220]