Difference between revisions of "Isopiperitenol dehydrogenase: Detailed description of the kinetics"

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(→‎Detailed description of the values used for the IPDH model)
(→‎Detailed description of the values used for the IPDH model)
 
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| [[file: 07_IPDH_substrate_concentrations_McConkey00.png |400px | An excerpt from McConkey et. al. 2000, Materials and Methods section]]
 
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| Assays were performed similarly to Kjonaas et. al (1985) <ref name="Kjonaas1985"></ref>, but used different susbtrate concentrations.
 
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<ref name="McConkey2000"> McConkey et.al., 2000. [https://www.researchgate.net/publication/12683615_Developmental_regulation_of_monoterpene_biosynthesis_in_the_glandular_trichomes_of_peppermint "Developmental regulation of monoterpene biosynthesis in the glandular trichomes of Peppermint", Plant Physiology, 122:215-223]</ref>
 
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| Assays were performed similarly to Kjonaas et. al (1985) <ref name="Kjonaas1985"></ref>, but used different susbtrate concentrations.
 
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[[file: 07_IPDH_substrate_concentrations_McConkey00.png |400px | An excerpt from McConkey et. al. 2000, Materials and Methods section]]
 
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[[file: 08_IPDH_substrate_conc_Kjonaas85.png |300px | An excerpt from Kjonaas et. al. 1985, Materials and Methods section]]
 
[[file: 08_IPDH_substrate_conc_Kjonaas85.png |300px | An excerpt from Kjonaas et. al. 1985, Materials and Methods section]]
 
|<ref name= "Kjonaas1985"> </ref>
 
|<ref name= "Kjonaas1985"> </ref>
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| [Isopiperitenol]
 +
| 200
 +
| µM
 +
| Assays were performed similarly to Kjonaas et. al (1985) <ref name="Kjonaas1985"></ref>, but they have used different susbtrate concentrations.
 +
  [[file: 07_IPDH_substrate_concentrations_McConkey00.png |400px | An excerpt from McConkey et. al. 2000, Materials and Methods section]]
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| <ref name="McConkey2000"> McConkey et.al., 2000. [https://www.researchgate.net/publication/12683615_Developmental_regulation_of_monoterpene_biosynthesis_in_the_glandular_trichomes_of_peppermint "Developmental regulation of monoterpene biosynthesis in the glandular trichomes of Peppermint", Plant Physiology, 122:215-223]</ref>
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|-
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| [NADP]
 +
| 1
 +
| mM
 +
| Assays were performed similarly to Kjonaas et. al (1985) <ref name="Kjonaas1985"></ref>, but they have used different susbtrate concentrations.
 +
[[file: 07_IPDH_substrate_concentrations_McConkey00.png |400px | An excerpt from McConkey et. al. 2000, Materials and Methods section]]
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| <ref name= "McConkey2000"> </ref>
 
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Latest revision as of 17:27, 16 March 2016

Back to terpene model MAIN page.

Back to the IPDH model page.

Detailed description of the values used for the IPDH model

Parameter Value Units Description References
[Isopiperitenol] 600 µM Enzyme assay values.

An excerpt from Kjonaas et. al. 1985, Materials and Methods section

[1]
[NAD] 1 mM Enzyme assay values.

An excerpt from Kjonaas et. al. 1985, Materials and Methods section

[1]
[Isopiperitenol] 200 µM Assays were performed similarly to Kjonaas et. al (1985) [1], but they have used different susbtrate concentrations. 
 An excerpt from McConkey et. al. 2000, Materials and Methods section
[2]
[NADP] 1 mM Assays were performed similarly to Kjonaas et. al (1985) [1], but they have used different susbtrate concentrations.

An excerpt from McConkey et. al. 2000, Materials and Methods section

[2]

References

  1. ↑ 1.0 1.1 1.2 1.3 Kjonaas R.B. et.al., 1985. "Metabolism of monoterpenes: Oxidation of isopiperitenol to isopiperitenone, and subsequent isomerization to piperitenone by soluble enzyme preparations from Peppermint (Mentha piperita) leaves", Archives of Biochemistry and Biophysics, 238(1):49-60 Cite error: Invalid <ref> tag; name "Kjonaas1985" defined multiple times with different content
  2. ↑ 2.0 2.1 McConkey et.al., 2000. "Developmental regulation of monoterpene biosynthesis in the glandular trichomes of Peppermint", Plant Physiology, 122:215-223 Cite error: Invalid <ref> tag; name "McConkey2000" defined multiple times with different content
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