Glyceraldehyde-3-phosphate dehydrogenase

This enzyme serves two functions in this step. First the enzyme transfers a hydrogen (H-) from glyceraldehyde phosphate to the oxidizing agent nicotinamide adenine dinucleotide (NAD+) to form NADH. Next it adds a phosphate (P) from the cytosol to the oxidized glyceraldehyde phosphate to form 1, 3-bisphosphoglycerate.

Chemical equation

$NAD + G3P + Pi \rightleftharpoons 1,3BPG + NADH$

Rate equation

v = \frac{ V_{mf}\frac{[A][B][C]}{K_aK_bK_c} - V_{mr} \frac{[P][Q]}{K_pK_q} }{1 + \frac{[A]}{K_a} + \frac{[A][B]}{K_aK_b} + \frac{[A][B][C]}{K_aK_bK_c} + \frac{[P][Q]}{K_pK_q} + +\frac{[Q]}{K_q} }