Difference between revisions of "Dehydrogenase"
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A '''dehydrogenase''' is an [http://en.wikipedia.org/wiki/Enzyme enzyme] that [http://en.wikipedia.org/wiki/Oxidize oxidize]s a substrate by a reduction reaction that transfers one or more hydrides (H<sup>−</sup>) to an electron acceptor, usually Nicotinamide adenine dinucleotide NAD<sup>+</sup>/NADP. | A '''dehydrogenase''' is an [http://en.wikipedia.org/wiki/Enzyme enzyme] that [http://en.wikipedia.org/wiki/Oxidize oxidize]s a substrate by a reduction reaction that transfers one or more hydrides (H<sup>−</sup>) to an electron acceptor, usually Nicotinamide adenine dinucleotide NAD<sup>+</sup>/NADP. | ||
Revision as of 11:07, 15 May 2014
A dehydrogenase is an enzyme that oxidizes a substrate by a reduction reaction that transfers one or more hydrides (H−) to an electron acceptor, usually Nicotinamide adenine dinucleotide NAD+/NADP.
Contents
Chemical reaction
Rate equation
Reversible mass action rate law is used
Parameters
Parameter | Value | Organism | Remarks |
---|---|---|---|
250 [1] | HeLa cell line | ||
1[1] |
Parameters with uncertainty
- This is a pseudo-reaction, modelled using mass action kinetics (i.e., as non-saturable, non-enzymatic reactions) to maintain maximal compatibility with Pyridine Nucleotides balances. The parameter values were adjusted through model simulations and the values chosen were those that best predicted NAD+ concentration (determined experimentally) in Hernandez et. al. [1]. No information is available about the uncertainty of these parameters. As these parameters are strictly positive, they are sampled using a log-normal distribution as are and values. The means are set to the value using fitted by Hernandez et al. [1] for the fixed-parameter model. The sampling of the parameters are made to fall within to allow a large exploration of the parameter space.
Parameter | Value | Organism | Remarks |
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Sampled between 0.25 and 250000 | HeLa cell line | ||
Sampled between 0.001 and 1000 |