Phosphoglucomutase

Phosphoglucomutase (PGLM) facilitates the interconversion of glucose 1-phosphate and glucose 6-phosphate by transfering a phosphate group on an α-D-glucose monomer from the 1' to the 6' position in the forward direction or the 6' to the 1' position in the reverse direction.

Chemical reaction

$Glc6P \rightleftharpoons Glc1P$

Rate equation

Mono substrate reversible Michelis-Menten with Haldane substitution is used as a rate law.

$\frac{\frac{V_{max}}{Km_{Glc6P}}\left( [Glc6P] - \frac{[Glc1P]}{K_{eq}} \right) }{1 + \frac{[Glc6P]}{Km_{Glc6P}} + \frac{[Glc1P]}{Km_{Glc1P}} }$

Parameter values

Parameter	Value	Units	Organism	Remarks
$V_{max}$	$2078$	$\text{mM min}^{-1}$	Rabbit muscle
$Km_{Glc6P}$	$5.7 \times 10^{-2}$	mM	Rabbit muscle
$Km_{Glc1P}$	$0.01054$	mM	Rabbit muscle
$K_{eq}$	$17.8$	mM	Rabbit muscle

Parameters with uncertainty

• The value of $Km_{Glc6P}$ using traditional meethods vary in between 0.03 - 0.05 mM. Using these two values as the maximum and the minimum we calculate the mean and standard deviation as $0.04 \pm 0.005$

Parameter	Value	Units	Organism	Remarks
$V_{max}$	$2078$	$\text{mM min}^{-1}$	Rabbit muscle
$Km_{Glc6P}$	$0.04 \pm 0.005$	mM	Rabbit muscle
$Km_{Glc1P}$	$0.01054 \pm 0.0009$	mM	Rabbit muscle