Difference between revisions of "Monocarboxylate (Lactate) transporter"
(→Parameters with uncertainty) |
|||
| Line 45: | Line 45: | ||
==Parameters with uncertainty== | ==Parameters with uncertainty== | ||
| − | * Among different isoforms the MCT1 in Tumour cells are considered in this model. The <math>Km_{Lactate_{in}}</math> is defined as <math>4.38 \pm 1.81</math> and <math>V_{mf} = 2.5 \pm 0.48</math> <ref name="manning_2000"></ref> | + | * Among different isoforms the MCT1 in Tumour cells are considered in this model. The <math>Km_{Lactate_{in}}</math> is defined as <math>4.38 \pm 1.81</math> and <math>V_{mf} = 2.5 \pm 0.48</math> <ref name="manning_2000"></ref>. |
* In the legend of Table-1 of <ref name="jackson_1996"></ref> it states Km and Vmax values for monocarboxylate transport into mouse Ehrlich Lettre ́ tumor cells were taken in to consideration. Therefore we consider these values to be for <math>Km_{Lactate_{out}}</math> and <math>V_{mr}</math>. Here the values are given in <math>mean \pm S.E.</math> with 7 experiments. Calculating Std. Dev. from S.E gives, <math>4.54 \pm 1.93</math> for <math>Km_{Lactate_{out}}</math> and <math>0.0207 \pm 0.003</math> for <math>V_{mr}</math>. | * In the legend of Table-1 of <ref name="jackson_1996"></ref> it states Km and Vmax values for monocarboxylate transport into mouse Ehrlich Lettre ́ tumor cells were taken in to consideration. Therefore we consider these values to be for <math>Km_{Lactate_{out}}</math> and <math>V_{mr}</math>. Here the values are given in <math>mean \pm S.E.</math> with 7 experiments. Calculating Std. Dev. from S.E gives, <math>4.54 \pm 1.93</math> for <math>Km_{Lactate_{out}}</math> and <math>0.0207 \pm 0.003</math> for <math>V_{mr}</math>. | ||
Revision as of 16:41, 2 May 2014
Monocarboxylate transporters,[1] or MCTs, constitute a family of proton-linked plasma membrane transporters that carry molecules having one carboxylate group (monocarboxylates), such as lactate and pyruvate.
Contents
Chemical equation
Rate equation
Reversible Michaelis-Menten rate law is used
Parameter values
| Parameter | Value | Units | Organism | Remarks |
|---|---|---|---|---|
|
2.5 [2] | 
|
Human tumor cells | |
|
2.4 [3] |
|
Rat liver cells | |
|
4.5 [3] | mM | Rat liver cell | |
|
4.54 [2] | mM | Human tumor cells |
Parameters with uncertainty
- Among different isoforms the MCT1 in Tumour cells are considered in this model. The is defined as
and 
[2].
- In the legend of Table-1 of [3] it states Km and Vmax values for monocarboxylate transport into mouse Ehrlich Lettre ́ tumor cells were taken in to consideration. Therefore we consider these values to be for and . Here the values are given in
with 7 experiments. Calculating Std. Dev. from S.E gives, 
for and 
for .
| Parameter | Value | Units | Organism | Remarks |
|---|---|---|---|---|
|
|
|
|
Human tumor cells | |
|
|
|
|
Human Tumor cell | |
|
|
Failed to parse (Cannot store math image on filesystem.): 4.38 \pm 1.81 (6) | mM | Human tumour cell | |
|
|
|
mM | Human tumor cells |
References
- ↑ Halestrap AP, Meredith D (2004). The SLC16 gene family-from monocarboxylate transporters (MCTs) to aromatic amino acid transporters and beyond. Pflugers Arch. 447 (5): 619–28 (doi)
- ↑ 2.0 2.1 2.2 Manning Fox JE et. al. (2000). Characterisation of human monocarboxylate transporter 4 substantiates its role in lactic acid efflux from skeletal muscle. J Physiol 529(Pt2):285-293
- ↑ 3.0 3.1 3.2 Jackson VN, Halestrap AP (1996) The kinetics, substrate, and inhibitor specificity of the monocarboxylate (lactate) transporter of rat liver cells determined using the fluorescent intracellular pH indicator, 2’,7’-bis(carboxyethyl)-5(6)-carboxyfluorescein. J Biol Chem 271:861–868
