Difference between revisions of "Lactate dehydrogenase"
(→Parameters with uncertainty) |
(→Parameters with uncertainty) |
||
Line 54: | Line 54: | ||
* Reported values of <math>Km_{PYR}</math> are 0.03 <ref>LeVan K.M., Goldberg E. (1991), ''Properties of human testis-specific lactate dehydrogenase expressed from Escherichia coli'', Biochem. J. 273, 587-592 (1991)</ref>, 0.398 <ref>Pettit S.M., Nealon D.A., Henderson A.R. (1981), ''Purification of lactate dehydrogenase isoenzyme-5 from human liver'', Clin. Chem. 27, 88-93 (1981)</ref>, 0.3 <ref name="Hernandez2011"></ref>. The mean value with std. dev. is <math>0.24 \pm 0.19</math>. | * Reported values of <math>Km_{PYR}</math> are 0.03 <ref>LeVan K.M., Goldberg E. (1991), ''Properties of human testis-specific lactate dehydrogenase expressed from Escherichia coli'', Biochem. J. 273, 587-592 (1991)</ref>, 0.398 <ref>Pettit S.M., Nealon D.A., Henderson A.R. (1981), ''Purification of lactate dehydrogenase isoenzyme-5 from human liver'', Clin. Chem. 27, 88-93 (1981)</ref>, 0.3 <ref name="Hernandez2011"></ref>. The mean value with std. dev. is <math>0.24 \pm 0.19</math>. | ||
+ | |||
+ | * The value for <math>Km_{NAD}</math> and <math>Km_{NADH}</math> has been reported for Ovine (Sheep) as <math>Km_{NAD} = 3.96 \pm 0.17</math> and <math>Km_{NADH} = 0.097 \pm 0.020</math> <ref>M. Doughty (1998), ''Some kinetic properties of lactate dehydrogenase activity in cell extracts from a mammalian (ovine) corneal epithelium'', Exp. Eye Res., 66, pp. 231–239</ref>. Due to lack of data in Human cells these two values are considered in our model. | ||
{|class="wikitable" | {|class="wikitable" | ||
Line 84: | Line 86: | ||
|- | |- | ||
|<math>Km_{NAD}</math> | |<math>Km_{NAD}</math> | ||
− | |0. | + | |<math>3.96 \pm 0.17</math> |
|mM | |mM | ||
|HeLa cell line | |HeLa cell line | ||
|- | |- | ||
|<math>Km_{NADH}</math> | |<math>Km_{NADH}</math> | ||
− | |0. | + | |<math>0.097 \pm 0.020</math> |
|mM | |mM | ||
|HeLa cell line | |HeLa cell line |
Revision as of 12:16, 2 May 2014
A dehydrogenase is an enzyme that transfers a hydride from one molecule to another. Lactate dehydrogenase catalyzes the conversion of pyruvate to lactate and back, as it converts NADH to NAD+ and back.
Contents
Chemical reactions
Rate equation
RAndom Bi-Bi reversible Michaelis-Menten equation is used. [1]
Prameter values
Parameter | Value | Units | Organism | Remarks |
---|---|---|---|---|
3.4 [1] | HeLa cell line | |||
0.54 | HeLa cell line | |||
0.1 | mM | HeLa cell line | ||
4.7 | mM | Rat AS-30D hepatoma | ||
0.07 | mM | HeLa cell line | ||
0.002 | mM | HeLa cell line |
Parameters with uncertainty
- Mean and Std. Dev. for has been reported in Table S3 for Marín-Hernández (2011) et. al. [1]. The Std. Dev. for is calculated based on the same ratio for .
- The value for and has been reported for Ovine (Sheep) as and [4]. Due to lack of data in Human cells these two values are considered in our model.
Parameter | Value | Units | Organism | Remarks |
---|---|---|---|---|
Failed to parse (Cannot store math image on filesystem.): 3.4 \pm 0.5 (3) [5] | HeLa cell line | |||
HeLa cell line | ||||
mM | HeLa cell line | |||
4.7 | mM | Rat AS-30D hepatoma | ||
mM | HeLa cell line | |||
mM | HeLa cell line |
References
- ↑ 1.0 1.1 1.2 1.3 Marín-Hernández A, Gallardo-Pérez JC, Rodríguez-Enríquez S et al (2011) Modeling cancer glycolysis. Biochim Biophys Acta 1807:755–767 (doi)
- ↑ LeVan K.M., Goldberg E. (1991), Properties of human testis-specific lactate dehydrogenase expressed from Escherichia coli, Biochem. J. 273, 587-592 (1991)
- ↑ Pettit S.M., Nealon D.A., Henderson A.R. (1981), Purification of lactate dehydrogenase isoenzyme-5 from human liver, Clin. Chem. 27, 88-93 (1981)
- ↑ M. Doughty (1998), Some kinetic properties of lactate dehydrogenase activity in cell extracts from a mammalian (ovine) corneal epithelium, Exp. Eye Res., 66, pp. 231–239
- ↑ Marín-Hernández A , Rodríguez-Enríquez S, Vital-González P A, et al. (2006). Determining and understanding the control of glycolysis in fast-growth tumor cells. Flux control by an over-expressed but strongly product-inhibited hexokinase. FEBS J., 273 , pp. 1975–1988(doi)