Difference between revisions of "Lactate dehydrogenase"
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* Mean and Std. Dev. for <math>V_{mf}</math> has been reported in Table S3 for Marín-Hernández (2011) et. al. <ref name="Hernandez2011"></ref>. The Std. Dev. for <math>V_{mr}</math> is calculated based on the same ratio for <math>V_{mf}</math>. | * Mean and Std. Dev. for <math>V_{mf}</math> has been reported in Table S3 for Marín-Hernández (2011) et. al. <ref name="Hernandez2011"></ref>. The Std. Dev. for <math>V_{mr}</math> is calculated based on the same ratio for <math>V_{mf}</math>. | ||
+ | |||
+ | * Reported values of <math>Km_{PYR}</math> are 0.03 <ref>LeVan K.M., Goldberg E. (1991), ''Properties of human testis-specific lactate dehydrogenase expressed from Escherichia coli'', Biochem. J. 273, 587-592 (1991)</ref> | ||
{|class="wikitable" | {|class="wikitable" |
Revision as of 10:34, 2 May 2014
A dehydrogenase is an enzyme that transfers a hydride from one molecule to another. Lactate dehydrogenase catalyzes the conversion of pyruvate to lactate and back, as it converts NADH to NAD+ and back.
Contents
Chemical reactions
![NADH + PYR \rightleftharpoons Lactate_{in} + NAD^+](/wiki/images/math/e/1/e/e1eba3432a22de6d0161e8a0e3297261.png)
Rate equation
RAndom Bi-Bi reversible Michaelis-Menten equation is used. [1]
![\frac{V_{mf}\frac{[NADH][PYR]}{Km_{NADH} K_{PYR}} - V_{mr}\frac{[Lactate_{in}][NAD]}{Km_{Lactate_{in}} K_{NAD}}}{1 + \frac{[NADH]}{Km_{NADH}} + \frac{[PYR]}{Km_{PYR}} + \frac{[NADH][PYR]}{Km_{NADH} Km_{PYR}} + \frac{[Lactate_{in}][NAD]}{Km_{Lactate_{in}} Km_{NAD}} + \frac{[Lactate_{in}]}{Km_{Lactate_{in}}} + \frac{[PYR]}{Km_{PYR}} }](/wiki/images/math/4/b/2/4b2b29cf3850c4c61d11218132ecd486.png)
Prameter values
Parameter | Value | Units | Organism | Remarks |
---|---|---|---|---|
![]() |
3.4 [1] | ![]() |
HeLa cell line | |
![]() |
0.54 | ![]() |
HeLa cell line | |
![]() |
0.1 | mM | HeLa cell line | |
![]() |
4.7 | mM | Rat AS-30D hepatoma | |
![]() |
0.07 | mM | HeLa cell line | |
![]() |
0.002 | mM | HeLa cell line |
Parameters with uncertainty
- Mean and Std. Dev. for
has been reported in Table S3 for Marín-Hernández (2011) et. al. [1]. The Std. Dev. for
is calculated based on the same ratio for
.
- Reported values of
are 0.03 [2]
Parameter | Value | Units | Organism | Remarks |
---|---|---|---|---|
![]() |
Failed to parse (Cannot store math image on filesystem.): 3.4 \pm 0.5 (3) [3] | ![]() |
HeLa cell line | |
![]() |
![]() |
![]() |
HeLa cell line | |
![]() |
0.1 | mM | HeLa cell line | |
![]() |
4.7 | mM | Rat AS-30D hepatoma | |
![]() |
0.07 | mM | HeLa cell line | |
![]() |
0.002 | mM | HeLa cell line |
References
- ↑ 1.0 1.1 1.2 Marín-Hernández A, Gallardo-Pérez JC, Rodríguez-Enríquez S et al (2011) Modeling cancer glycolysis. Biochim Biophys Acta 1807:755–767 (doi)
- ↑ LeVan K.M., Goldberg E. (1991), Properties of human testis-specific lactate dehydrogenase expressed from Escherichia coli, Biochem. J. 273, 587-592 (1991)
- ↑ Marín-Hernández A , Rodríguez-Enríquez S, Vital-González P A, et al. (2006). Determining and understanding the control of glycolysis in fast-growth tumor cells. Flux control by an over-expressed but strongly product-inhibited hexokinase. FEBS J., 273 , pp. 1975–1988(doi)