Enolase

Enolase, also known as phosphopyruvate hydratase, catalysis the conversion of 2-phosphoglycerate (2-PG) to phosphoenolpyruvate (PEP). This is the penultimate step of glycolysis.

Chemical equation

$2PG \rightleftharpoons PEP$

Rate equation

Mono-substrate reversible Michaelis-Menten equation is used.

$\frac{V_{mf}\frac{[2PG]}{K_{2PG}}-V_{mr}\frac{[PEP]}{K_{PEP}}}{1 + \frac{[2PG]}{K_{2PG}} + \frac{[PEP]}{K_{PEP}}}$

Parameter values

Parameter	Value	Units	Organism	Remarks
$V_{mf}$	0.34	$\text{mM min}^{-1}$	HeLa cell line
$V_{mr}$	0.38	$\text{mM min}^{-1}$
$Km_{2PG}$	0.038	mM
$Km_{PEP}$	0.06	mM

Parameters with uncertainty

• Three values for $Km_{2PG}$ is collected. The values are 0.20 , 0.199 , 0.038 . The mean and std. dev. is $0.145 \pm 0.07$

• Similarly for $Km_{PEP}$ three reported values are 0.58, 0.702, 0.06. The uncertainty is then $0.44 \pm 0.276$.

• In Pietkiewicz et. al. (2009) $V_{mr}$ is reported as 1.4 $mmol/min^{-1}$ and Marín-Hernández et. al. (2011) reported it to be 0.4. The mean and the std. dev. calculated from these two values are $0.9 \pm 0.5$.

Parameter	Value	Units	Organism	Remarks
$V_{mf}$	$0.36 \pm 0.15 (5)$	$\text{mM min}^{-1}$	HeLa cell line
$V_{mr}$	$0.9 \pm 0.5$	$\text{mM min}^{-1}$
$Km_{2PG}$	$0.145 \pm 0.07$	mM
$Km_{PEP}$	$0.44 \pm 0.276$	mM