Difference between revisions of "Enolase"
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| − | == | + | ==Parameters with uncertainty== |
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| + | {|class="wikitable" | ||
| + | ! Parameter | ||
| + | ! Value | ||
| + | ! Units | ||
| + | ! Organism | ||
| + | ! Remarks | ||
| + | |- | ||
| + | |<math>V_{mf}</math> | ||
| + | |0.34 <ref name="Hernandez_2006"> Marín-Hernández A , Rodríguez-Enríquez S, Vital-González P A, ''et al.'' (2006). ''Determining and understanding the control of glycolysis in fast-growth tumor cells. Flux control by an over-expressed but strongly product-inhibited hexokinase''. FEBS J., 273 , pp. 1975–1988([http://dx.doi.org/doi:10.1111/j.1742-4658.2006.05214.x doi]) </ref> | ||
| + | |<math> \text{mM min}^{-1} </math> | ||
| + | |rowspan="4"|HeLa cell line | ||
| + | |rowspan="4"| | ||
| + | |- | ||
| + | |<math>V_{mr}</math> | ||
| + | |0.38<ref name="Hernandez2011"></ref> | ||
| + | |<math> \text{mM min}^{-1} </math> | ||
| + | |- | ||
| + | |<math>Km_{2PG}</math> | ||
| + | |0.038<ref name="Hernandez2011"></ref> | ||
| + | |mM | ||
| + | |- | ||
| + | |<math>Km_{PEP}</math> | ||
| + | |0.06<ref name="Hernandez2011"></ref> | ||
| + | |mM | ||
| + | |} | ||
==References== | ==References== | ||
<references/> | <references/> | ||
Revision as of 14:48, 29 April 2014
Enolase, also known as phosphopyruvate hydratase, catalysis the conversion of 2-phosphoglycerate (2-PG) to phosphoenolpyruvate (PEP). This is the penultimate step of glycolysis.
Contents
Chemical equation
Rate equation
Mono-substrate reversible Michaelis-Menten equation is used. [1]
![\frac{V_{mf}\frac{[2PG]}{K_{2PG}}-V_{mr}\frac{[PEP]}{K_{PEP}}}{1 + \frac{[2PG]}{K_{2PG}} + \frac{[PEP]}{K_{PEP}}}](/wiki/images/math/6/a/9/6a9ca37a2fd76b4eb56a1e6a5cfb67d0.png)
Parameter values
| Parameter | Value | Units | Organism | Remarks |
|---|---|---|---|---|
|
0.34 [2] | 
|
HeLa cell line | |
|
0.38[1] |
| ||
|
0.038[1] | mM | ||
|
0.06[1] | mM |
Parameters with uncertainty
| Parameter | Value | Units | Organism | Remarks |
|---|---|---|---|---|
|
|
0.34 [2] |
|
HeLa cell line | |
|
|
0.38[1] |
| ||
|
|
0.038[1] | mM | ||
|
|
0.06[1] | mM |
References
- ↑ 1.0 1.1 1.2 1.3 1.4 1.5 1.6 Marín-Hernández A, Gallardo-Pérez JC, Rodríguez-Enríquez S et al (2011) Modeling cancer glycolysis. Biochim Biophys Acta 1807:755–767 (doi)
- ↑ 2.0 2.1 Marín-Hernández A , Rodríguez-Enríquez S, Vital-González P A, et al. (2006). Determining and understanding the control of glycolysis in fast-growth tumor cells. Flux control by an over-expressed but strongly product-inhibited hexokinase. FEBS J., 273 , pp. 1975–1988(doi)
