Difference between revisions of "3-phosphoglycerate mutase"

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|0.94 <ref name="Hernandez_2006"> Marín-Hernández A , Rodríguez-Enríquez S, Vital-González P A, ''et al.'' (2006). ''Determining and understanding the control of glycolysis in fast-growth tumor cells. Flux control by an over-expressed but strongly product-inhibited hexokinase''. FEBS J., 273 , pp. 1975–1988([http://dx.doi.org/doi:10.1111/j.1742-4658.2006.05214.x doi]) </ref>
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|<math>1.4 \pm 1</math> <ref name="Hernandez_2006"> Marín-Hernández A , Rodríguez-Enríquez S, Vital-González P A, ''et al.'' (2006). ''Determining and understanding the control of glycolysis in fast-growth tumor cells. Flux control by an over-expressed but strongly product-inhibited hexokinase''. FEBS J., 273 , pp. 1975–1988([http://dx.doi.org/doi:10.1111/j.1742-4658.2006.05214.x doi]) </ref>
 
| <math> \text{mM min}^{-1} </math>
 
| <math> \text{mM min}^{-1} </math>
 
|rowspan="4"|HeLa cell line
 
|rowspan="4"|HeLa cell line

Revision as of 12:48, 29 April 2014

Phosphoglycerate mutase (PGAM) is an enzyme that catalyzes the internal transfer of a phosphate group from C-3 to C-2 which results in the conversion of 3-phosphoglycerate (3PG) to 2-phosphoglycerate (2PG).

Chemical equation

3PG \rightleftharpoons 2PG

Rate equation

Mono-substrate reversible Michaelis-Menten equation is used. [1]

\frac{V_{mf}\frac{[3PG]}{Km_{3PG}}-V_{mr}\frac{[2PG]}{Km_{2PG}}}{1 + \frac{[3PG]}{Km_{3PG}} + \frac{[2PG]}{Km_{2PG}}}

Parameter values

Parameter Value Units Organism Remarks
V_{mf} 0.94 [2] \text{mM min}^{-1} HeLa cell line
V_{mr} 0.36 [2] \text{mM min}^{-1}
Km_{3PG} 0.19[1] mM
Km_{2PG} 0.12[1] mM

Parameters with uncertainty

Parameter Value Units Organism Remarks
V_{mf} 1.4 \pm 1 [2] \text{mM min}^{-1} HeLa cell line
V_{mr} 0.36 [2] \text{mM min}^{-1}
Km_{3PG} 0.19[1] mM
Km_{2PG} 0.12[1] mM

References

  1. ↑ 1.0 1.1 1.2 1.3 1.4 Marín-Hernández A, Gallardo-Pérez JC, Rodríguez-Enríquez S et al (2011) Modeling cancer glycolysis. Biochim Biophys Acta 1807:755–767 (doi)
  2. ↑ 2.0 2.1 2.2 2.3 Marín-Hernández A , Rodríguez-Enríquez S, Vital-González P A, et al. (2006). Determining and understanding the control of glycolysis in fast-growth tumor cells. Flux control by an over-expressed but strongly product-inhibited hexokinase. FEBS J., 273 , pp. 1975–1988(doi)
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