Difference between revisions of "3-phosphoglycerate kinase"

Revision as of 12:26, 29 April 2014

3-Phosphoglycerate kinase (PGK) is an enzyme that catalyzes the reversible transfer of a phosphate group from 1,3-bisphosphoglycerate (1,3-BPG) to ADP producing 3-phosphoglycerate (3-PG) and ATP. Like all kinases it is a transferase.

1,3BPG + ADP \rightleftharpoons 3PG + ATP

Random Bi-Bi reversible Michaelis-Menten euation for non-interacting substrates are used. ^[1]

\frac{V_{mf}\frac{[1,3BPG][ADP]}{K_{1,3BPG} K_{ADP}} - V_{mr}\frac{[3PG][ATP]}{K_{3PG} K_{ATP}}}{1 + \frac{[1,3BPG]}{K_{1,3BPG}} + \frac{[ADP]}{K_{ADP}} + \frac{[1,3BPG][ADP]}{K_{1,3BPG} K_{ADP}} + \frac{[3PG][ATP]}{K_{3PG} K_{ATP}} + \frac{[3PG]}{K_{3PG}} + \frac{[ADP]}{K_{ADP}} }

Parameter	Value	Units	Organism
$V_{mf}$	8.7 ^[2]	$mM \times min^{-1}$	HeLa cell line
$V_{mr}$	2.5^[1]	$mM \times min^{-1}$
$Km_{1,3BPG}$	0.079^[1]	mM
$Km_{3PG}$	0.13^[1]	mM
$Km_{ADP}$	0.04^[1]	mM
$Km_{ATP}$	0.27^[1]	mM

Parameter	Value	Units	Organism
$V_{mf}$	Failed to parse (Cannot store math image on filesystem.): 13 \pm 6 ^[2]	$mM \times min^{-1}$	HeLa cell line
$V_{mr}$	2.5^[1]	$mM \times min^{-1}$
$Km_{1,3BPG}$	$0.0005 \pm 0.0002$ ^[3]	mM
$Km_{3PG}$ ^[3]	$0.05 \pm 0.02$	mM
$Km_{ADP}$	$0.12 \pm 0.02$ ^[3]	mM
$Km_{ATP}$	$0.11 \pm 0.02$ ^[3]	mM

↑ ^1.0 ^1.1 ^1.2 ^1.3 ^1.4 ^1.5 ^1.6 Marín-Hernández A, Gallardo-Pérez JC, Rodríguez-Enríquez S et al (2011) Modeling cancer glycolysis. Biochim Biophys Acta 1807:755–767 (doi)
↑ ^2.0 ^2.1 Marín-Hernández A , Rodríguez-Enríquez S, Vital-González P A, et al. (2006). Determining and understanding the control of glycolysis in fast-growth tumor cells. Flux control by an over-expressed but strongly product-inhibited hexokinase. FEBS J., 273 , pp. 1975–1988(doi)
↑ ^3.0 ^3.1 ^3.2 ^3.3 Szabo, J.; Varga, A.; Flachner, B.; Konarev, P.V.; Svergun, D.I.; Zavodszky, P.; Vas, M. (2008), Role of side-chains in the operation of the main molecular hinge of 3-phosphoglycerate kinase, FEBS Lett. 582, 1335-1340

@@ Line 70: / Line 70: @@
 |mM
 |-
-|<math>Km_{3PG}</math>
+|<math>Km_{3PG}</math><ref name="Szabo_2008"></ref>
 |<math>0.05 \pm 0.02</math>
 |mM
 |-
 |<math>Km_{ADP}</math>
-|<math>0.12 \pm 0.02</math>
+|<math>0.12 \pm 0.02</math><ref name="Szabo_2008"></ref>
 |mM
 |-
 |<math>Km_{ATP}</math>
-|<math>0.11 \pm 0.02</math>
+|<math>0.11 \pm 0.02</math><ref name="Szabo_2008"></ref>
 |mM
 |}