Revision as of 15:48, 28 April 2014

This enzyme splits fructose 1, 6-bisphosphate into two sugars that are isomers of each other. These two sugars are Dihydroxyacetone phosphate (DHAP) and Glyceraldehyde 3-phosphate (Gly3P).

Chemical equation

Fru1,6BP \rightleftharpoons Gly3P + DHAP

Reversible Uni-Bi Michaelis-Menten is used. ^[1]

\frac{V_{mf} \frac{[Fru1,6BP]}{K_{Fru1,6BP}} - V_{mr}\frac{[DHAP][G3P]}{K_{DHAP}K_{Gly3P}} }{1 + \frac{[Fru1,6BP]}{K_{Fru1,6BP}} + \frac{[DHAP]}{K_{DHAP}} +\frac{[Gly3P]}{K_{Gly3P}} + \frac{[DHAP][Gly3P]}{K_{DHAP}K_{Gly3P}} }

Parameter	Value	Units	Organism
$V_{mf}$	0.08 ^[2]	$mM \times min^{-1}$	Hela cell line
$V_{mr}$	0.063^[2]	$mM \times min^{-1}$	Rodent AS-30D hepatoma
$Km_{Fru1,6BP}$	0.009^[2]	mM	Hela cell line
$Km_{Gly3P}$	0.16^[2]	mM	Rodent AS-30D hepatoma
$Km_{DHAP}$	0.08^[2]	mM	Rodent AS-30D hepatoma

The value for V_{mf} is collected from Hernandez et. al. ^[2]. The V_{mr} is calcualted from the sampled $V_{mr}$ , $Km_{Gly3P}$ , $Km_{DHAP}$ and $K_{eq}$ values using the Haldane equation .

The value for $Km_{Fru1,6BP}, Km_{Gly3P}, Km_{DHAP}$ are collected from Ali D. Malay et. al. ^[3] for wildtype Aldolase B gene at $30^{\circ}$ C.

Parameter	Value	Units	Organism
$V_{mf}$	$0.2 \pm 0.05 (5)$ ^[2]	$mM \times min^{-1}$	Hela cell line
$V_{mr}$	Sampled based on Haldane equation	$mM \times min^{-1}$
$Km_{Fru1,6BP}$	$0.0024 \pm 0.0004$	mM	Human cell
$Km_{Gly3P}$	$0.48 \pm 0.15$	mM	Human cell
$Km_{DHAP}$	$0.38 \pm 0.01$	mM	Human cell

Equilibrium constant	Conditions	Source
0.10	pH=7, T=25°C	Voet et al.^[4] from Newshole et al. (1973) ^[5]p 97: $\Delta G' = 22.8\ kJ.mol^{-1}$ , $Keq = exp(-\frac{\Delta G'}{RT}) = exp(\frac{-22800}{8.31*298.15}) \approx 0.10$
0.067	pH=7, T=25°C	Lehninger, (1975)^[6] p 407: $\Delta G' = 23.8\ kJ.mol^{-1}$ , $Keq = exp(-\frac{\Delta G'}{RT}) = exp(\frac{-23800}{8.31*298.15}) \approx 0.067$

↑ Marín-Hernández A, Gallardo-Pérez JC, Rodríguez-Enríquez S et al (2011) Modeling cancer glycolysis. Biochim Biophys Acta 1807:755–767 (doi)
↑ ^2.0 ^2.1 ^2.2 ^2.3 ^2.4 ^2.5 ^2.6 Marín-Hernández A , Rodríguez-Enríquez S, Vital-González P A, et al. (2006). Determining and understanding the control of glycolysis in fast-growth tumor cells. Flux control by an over-expressed but strongly product-inhibited hexokinase. FEBS J., 273 , pp. 1975–1988(doi)
↑ Malay AD, Procious SL, Tolan DR. (2002). The temperature dependence of activity and structure for most prevalent mutant aldolase B associated with hereditary fructose intolerance, Arch BiochemBiophys 408: 295–304.
↑ Voet, D., Voet., J.G. and Pratt, C. W. (1999) Fundamentals of biochemistry, Wiley
↑ Newshole, E.A. and Stuart, C. (1973) Regulation in Metabolism, Wiley
↑ Lehninger, A.L. (1975) Biochemistry (2nd edn), Worth

@@ Line 63: / Line 63: @@
 |-
 |<math>V_{mr}</math>
-|0.063
+|Sampled based on Haldane equation
 |<math> mM \times min^{-1} </math>
 |
@@ Line 81: / Line 81: @@
 |mM
 |Human cell
+|}
+= Equilibrium constant ==
+{| border="1" cellpadding="2"
+! Equilibrium constant
+! Conditions
+! Source
+|-
+| 0.10
+| pH=7, T=25°C
+| Voet et al.<ref name="voet">Voet, D., Voet., J.G. and Pratt, C. W. (1999) Fundamentals of biochemistry, Wiley</ref> from Newshole et al. (1973) <ref name="newshole73">Newshole, E.A. and Stuart, C. (1973) Regulation in Metabolism, Wiley</ref>p 97:<br/>
+<math>\Delta G' = 22.8\ kJ.mol^{-1}</math>, <math>Keq = exp(-\frac{\Delta G'}{RT}) = exp(\frac{-22800}{8.31*298.15}) \approx 0.10</math>
+|-
+| 0.067
+| pH=7, T=25°C
+| Lehninger, (1975)<ref name="lehninger75">Lehninger, A.L. (1975) Biochemistry (2nd edn), Worth</ref> p 407:<br/>
+<math>\Delta G' = 23.8\ kJ.mol^{-1}</math>, <math>Keq = exp(-\frac{\Delta G'}{RT}) = exp(\frac{-23800}{8.31*298.15}) \approx 0.067</math>
 |}
 ==References==
 <references/>