Difference between revisions of "Fructose 1,6 bisphosphate aldolase"

Revision as of 15:23, 28 April 2014

This enzyme splits fructose 1, 6-bisphosphate into two sugars that are isomers of each other. These two sugars are Dihydroxyacetone phosphate (DHAP) and Glyceraldehyde 3-phosphate (Gly3P).

Fru1,6BP \rightleftharpoons Gly3P + DHAP

Reversible Uni-Bi Michaelis-Menten is used. ^[1]

\frac{V_{mf} \frac{[Fru1,6BP]}{K_{Fru1,6BP}} - V_{mr}\frac{[DHAP][G3P]}{K_{DHAP}K_{Gly3P}} }{1 + \frac{[Fru1,6BP]}{K_{Fru1,6BP}} + \frac{[DHAP]}{K_{DHAP}} +\frac{[Gly3P]}{K_{Gly3P}} + \frac{[DHAP][Gly3P]}{K_{DHAP}K_{Gly3P}} }

Parameter	Value	Units	Organism
$V_{mf}$	0.08 ^[2]	$mM \times min^{-1}$	Hela cell line
$V_{mr}$	0.063^[2]	$mM \times min^{-1}$	Rodent AS-30D hepatoma
$Km_{Fru1,6BP}$	0.009^[2]	mM	Hela cell line
$Km_{Gly3P}$	0.16^[2]	mM	Rodent AS-30D hepatoma
$Km_{DHAP}$	0.08^[2]	mM	Rodent AS-30D hepatoma

The value for V_{mf} is collected from Hernandez et. al. ^[2]. The V_{mr} is calcualted from the sampled $V_{mr}$ , $Km_{Gly3P}$ , $Km_{DHAP}$ and $K_{eq}$ values using the Haldane equation .

The value for $Km_{Fru1,6BP}, Km_{Gly3P}, Km_{DHAP}$ are collected from Ali D. Malay et. al. ^[3] for wildtype Aldolase B gene at $30^{\circ}$ C.

Parameter	Value	Units	Organism
$V_{mf}$	$0.2 \pm 0.05 (5)$ ^[2]	$mM \times min^{-1}$	Hela cell line
$V_{mr}$	0.063	$mM \times min^{-1}$	Rodent AS-30D hepatoma
$Km_{Fru1,6BP}$	$0.0024 \pm 0.0004$	mM	Hela cell line
$Km_{Gly3P}$	$0.48 \pm 0.15$ ^[2]	mM	Rodent AS-30D hepatoma
$Km_{DHAP}$	$0.38 \pm 0.01$ ^[2]	mM	Rodent AS-30D hepatoma

↑ Marín-Hernández A, Gallardo-Pérez JC, Rodríguez-Enríquez S et al (2011) Modeling cancer glycolysis. Biochim Biophys Acta 1807:755–767 (doi)
↑ ^2.0 ^2.1 ^2.2 ^2.3 ^2.4 ^2.5 ^2.6 ^2.7 ^2.8 Marín-Hernández A , Rodríguez-Enríquez S, Vital-González P A, et al. (2006). Determining and understanding the control of glycolysis in fast-growth tumor cells. Flux control by an over-expressed but strongly product-inhibited hexokinase. FEBS J., 273 , pp. 1975–1988(doi)
↑ Malay AD, Procious SL, Tolan DR. (2002). The temperature dependence of activity and structure for most prevalent mutant aldolase B associated with hereditary fructose intolerance, Arch BiochemBiophys 408: 295–304.

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 |<math>Km_{Fru1,6BP}</math>
-|0.009<ref name="Hernandez_2006"></ref>
+|<math>0.0024 \pm 0.0004</math>
 |mM
 |Hela cell line
 |-
 |<math>Km_{Gly3P}</math>
-|0.16<ref name="Hernandez_2006"></ref>
+|<math>0.48 \pm 0.15</math><ref name="Hernandez_2006"></ref>
 |mM
 |Rodent AS-30D hepatoma
 |-
 |<math>Km_{DHAP}</math>
-|0.08<ref name="Hernandez_2006"></ref>
+|<math>0.38 \pm 0.01</math><ref name="Hernandez_2006"></ref>
 |mM
 |Rodent AS-30D hepatoma