Difference between revisions of "Fructose 1,6 bisphosphate aldolase"

Revision as of 15:19, 28 April 2014

This enzyme splits fructose 1, 6-bisphosphate into two sugars that are isomers of each other. These two sugars are Dihydroxyacetone phosphate (DHAP) and Glyceraldehyde 3-phosphate (Gly3P).

Fru1,6BP \rightleftharpoons Gly3P + DHAP

Reversible Uni-Bi Michaelis-Menten is used. ^[1]

\frac{V_{mf} \frac{[Fru1,6BP]}{K_{Fru1,6BP}} - V_{mr}\frac{[DHAP][G3P]}{K_{DHAP}K_{Gly3P}} }{1 + \frac{[Fru1,6BP]}{K_{Fru1,6BP}} + \frac{[DHAP]}{K_{DHAP}} +\frac{[Gly3P]}{K_{Gly3P}} + \frac{[DHAP][Gly3P]}{K_{DHAP}K_{Gly3P}} }

Parameter	Value	Units	Organism
$V_{mf}$	0.08 ^[2]	$mM \times min^{-1}$	Hela cell line
$V_{mr}$	0.063^[2]	$mM \times min^{-1}$	Rodent AS-30D hepatoma
$Km_{Fru1,6BP}$	0.009^[2]	mM	Hela cell line
$Km_{Gly3P}$	0.16^[2]	mM	Rodent AS-30D hepatoma
$Km_{DHAP}$	0.08^[2]	mM	Rodent AS-30D hepatoma

The value for V_{mf} is collected from Hernandez et. al. ^[2]. The V_{mr} is calcualted from the sampled $V_{mr}$ , $Km_{Gly3P}$ , $Km_{DHAP}$ and $K_{eq}$ values using the Haldane equation .

The value for $Km_{Fru1,6BP}, Km_{Gly3P}, Km_{DHAP}$ are collected for Aldolase B gene from Ali D. Malay et. al. ^[3].

Parameter	Value	Units	Organism
$V_{mf}$	$0.2 \pm 0.05 (5)$ ^[2]	$mM \times min^{-1}$	Hela cell line
$V_{mr}$	0.063	$mM \times min^{-1}$	Rodent AS-30D hepatoma
$Km_{Fru1,6BP}$	0.009^[2]	mM	Hela cell line
$Km_{Gly3P}$	0.16^[2]	mM	Rodent AS-30D hepatoma
$Km_{DHAP}$	0.08^[2]	mM	Rodent AS-30D hepatoma

↑ Marín-Hernández A, Gallardo-Pérez JC, Rodríguez-Enríquez S et al (2011) Modeling cancer glycolysis. Biochim Biophys Acta 1807:755–767 (doi)
↑ ^2.0 ^2.1 ^2.2 ^2.3 ^2.4 ^2.5 ^2.6 ^2.7 ^2.8 ^2.9 Marín-Hernández A , Rodríguez-Enríquez S, Vital-González P A, et al. (2006). Determining and understanding the control of glycolysis in fast-growth tumor cells. Flux control by an over-expressed but strongly product-inhibited hexokinase. FEBS J., 273 , pp. 1975–1988(doi)
↑ Malay AD, Procious SL, Tolan DR. (2002). The temperature dependence of activity and structure for most prevalent mutant aldolase B associated with hereditary fructose intolerance, Arch BiochemBiophys 408: 295–304.

@@ Line 46: / Line 46: @@
 ==Parameters with uncertainty==
+*The value for V_{mf} is collected from Hernandez ''et. al.'' <ref name="Hernandez_2006"></ref>. The V_{mr} is calcualted from the sampled <math>V_{mr}</math>, <math>Km_{Gly3P}</math>, <math>Km_{DHAP}</math> and <math>K_{eq}</math> values using the Haldane equation .
+*The value for <math>Km_{Fru1,6BP}, Km_{Gly3P}, Km_{DHAP}</math>  are collected for Aldolase B gene from Ali D. Malay ''et. al.'' <ref name="Ali">Malay AD, Procious SL, Tolan DR. (2002). ''The temperature dependence of activity and structure for most prevalent mutant aldolase B associated with hereditary fructose intolerance'', Arch BiochemBiophys 408: 295–304.</ref>.
 {|class="wikitable"
 ! Parameter
@@ Line 60: / Line 63: @@
 |-
 |<math>V_{mr}</math>
-|0.063<ref name="Hernandez_2006"></ref>
+|0.063
 |<math> mM \times min^{-1} </math>
 |Rodent AS-30D hepatoma