Difference between revisions of "Glucose Transporter"
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==Parameters with uncertainty== | ==Parameters with uncertainty== | ||
| − | Thirteen isoforms of Glucose transporter (GLUT) exist in Mammalian cells to facilitate the uptake of glucose from extracellular fluid into the cell. However, in most of the literature the kinetic properties of GLUT 1-4 isoforms are only reported | + | Thirteen isoforms of Glucose transporter (GLUT) exist in Mammalian cells to facilitate the uptake of glucose from extracellular fluid into the cell. However, in most of the literature the kinetic properties of GLUT 1-4 isoforms are only reported <ref name="Zhao_2007"> Zhao, F. and Keating, A. (2007), ''Functional Properties and Genomics of Glucose Transporters'', Curr Genomics, 8(2), 113-128</ref><ref name="Medina_2004">Medina RA, Meneses AM, Vera JC, Guzmán C, Nualart F, Rodriguez F, de los Angeles Garcia M, Kato S,Espinoza N, Monso C et al. (2004), ''Differential regulation of glucose transporter expression by estrogen and progesterone in Ishikawa endometrial cancer cells'', J Endocrinol 182, 467–478.</ref>. In our model <math>K_{m}</math> value for the forward reaction is taken from the paper ''Modeling cancer glycolysis''. The mean and std. dev. of <math>K_{m}</math> for the reverse reaction is being averaged from the 4 isoforms of GLUT reported in Zhao ''et. al.''<ref name="Zhao_2007">. |
==References== | ==References== | ||
<references/> | <references/> | ||
Revision as of 11:06, 23 April 2014
Glucose transporters are a wide group of membrane proteins that facilitate the transport of glucose over a plasma membrane. Glucose is a vital source of energy for all life.
Contents
Chemical equation
Rate equation
Mono-Substrate reversible Michaelis-Menten with Haldane substitution is used[1].
![v = \frac{V_{mf}\Big([Glucose_{out}] - \frac{[Glucose_{in}]}{K_{eq}}\Big)}{K_{Glucose_{out}}\Big(1 + \frac{[Glucose_{in}]}{K_{Glucose_{in}}}\Big) + [Glucose_{out}]}](/wiki/images/math/5/2/f/52f388bcf345df8a8dae68f7aa8cb456.png)
Parameter values
| Parameter | Value | Units | Organism | Remarks |
|---|---|---|---|---|
|
.017 [2] | 
|
HeLa cell line | Vm is converted to from U × (mg total cellular protein) at  C
|
|
1[3] | |||
|
9.3[2] | mM | ||
|
10[3] | mM |
Parameters with uncertainty
Thirteen isoforms of Glucose transporter (GLUT) exist in Mammalian cells to facilitate the uptake of glucose from extracellular fluid into the cell. However, in most of the literature the kinetic properties of GLUT 1-4 isoforms are only reported [4][5]. In our model 
value for the forward reaction is taken from the paper Modeling cancer glycolysis. The mean and std. dev. of for the reverse reaction is being averaged from the 4 isoforms of GLUT reported in Zhao et. al.<ref name="Zhao_2007">.
References
- ↑ Marín-Hernández A, Gallardo-Pérez JC, Rodríguez-Enríquez S et al (2011). Modeling cancer glycolysis. Biochim Biophys Acta, 1807:755–767 (doi)
- ↑ 2.0 2.1 Rodríguez-Enríquez S, Marín-Hernández A, Gallardo-Pérez J C, et. al.(2009). Kinetics of transport and phosphorylation of glucose in cancer cells. Journal of Cell. Physiology, 221 , pp. 552–559 (doi)
- ↑ 3.0 3.1 Arbitrary value
- ↑ Zhao, F. and Keating, A. (2007), Functional Properties and Genomics of Glucose Transporters, Curr Genomics, 8(2), 113-128
- ↑ Medina RA, Meneses AM, Vera JC, Guzmán C, Nualart F, Rodriguez F, de los Angeles Garcia M, Kato S,Espinoza N, Monso C et al. (2004), Differential regulation of glucose transporter expression by estrogen and progesterone in Ishikawa endometrial cancer cells, J Endocrinol 182, 467–478.
