Difference between revisions of "Lactate dehydrogenase"
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|<math>V_{mf}</math> | |<math>V_{mf}</math> | ||
| − | | | + | |3.4 <ref name="Hernandez2011"> Marín-Hernández A, Gallardo-Pérez JC, Rodríguez-Enríquez S et al (2011) Modeling cancer glycolysis. Biochim Biophys Acta 1807:755–767 ([http://dx.doi.org/10.1016/j.bbabio.2010.11.006 doi]) </ref> |
|<math> \text{mM min}^{-1} </math> | |<math> \text{mM min}^{-1} </math> | ||
|HeLa cell line | |HeLa cell line | ||
| Line 23: | Line 23: | ||
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|<math>V_{mr}</math> | |<math>V_{mr}</math> | ||
| − | | | + | |0.54<ref name="Hernandez2011"></ref> |
|<math> \text{mM min}^{-1} </math> | |<math> \text{mM min}^{-1} </math> | ||
|Rat AS-30D hepatoma | |Rat AS-30D hepatoma | ||
Revision as of 16:48, 28 March 2014
A dehydrogenase is an enzyme that transfers a hydride from one molecule to another. Lactate dehydrogenase catalyzes the conversion of pyruvate to lactate and back, as it converts NADH to NAD+ and back.
Chemical reactions
Rate equation
RAndom Bi-Bi reversible Michaelis-Menten equation is used. [1]
![\frac{V_{mf}\frac{[NADH][PYR]}{Km_{NADH} K_{PYR}} - V_{mr}\frac{[Lactate_{in}][NAD]}{Km_{Lactate_{in}} K_{NAD}}}{1 + \frac{[NADH]}{Km_{NADH}} + \frac{[PYR]}{Km_{PYR}} + \frac{[NADH][PYR]}{Km_{NADH} Km_{PYR}} + \frac{[Lactate_{in}][NAD]}{Km_{Lactate_{in}} Km_{NAD}} + \frac{[Lactate_{in}]}{Km_{Lactate_{in}}} + \frac{[PYR]}{Km_{PYR}} }](/wiki/images/math/4/b/2/4b2b29cf3850c4c61d11218132ecd486.png)
Prameter values
| Parameter | Value | Units | Organism | Remarks |
|---|---|---|---|---|
|
3.4 [1] | 
|
HeLa cell line | |
|
0.54[1] |
|
Rat AS-30D hepatoma | |
|
0.1[1] | mM | HeLa cell line | |
|
4.7[1] | mM | Rat AS-30D hepatoma | |
|
0.07[1] | mM | HeLa cell line | |
|
0.002[1] | mM | HeLa cell line |
