Difference between revisions of "Triosephosphate isomerase"

Revision as of 16:33, 28 March 2014

This enzyme rapidly inter-converts the molecules Dihydroxyacetone phosphate (DHAP) and Glyceraldehyde 3-phosphate (Gly3P). Gly3P is removed as soon as it is formed to be used in the next step of glycolysis.

DHAP \rightleftharpoons Gly3P

Reversible Michaelis-Menten is used ^[1]

v = \frac{ V_{mf}\frac{[DHAP]}{K_{DHAP}} - V_{mr}\frac{[Gly3P]}{K_{Gly3P}} }{1 + \frac{[DHAP]}{K_{DHAP}} + \frac{[Gly3P]}{K_{Gly3P}} }

Parameter	Value	Units	Organism
$V_{mf}$	3.4 ^[1]	$mM \times min^{-1}$	Hela cell line
$V_{mr}$	28^[2]	$mM \times min^{-1}$
$Km_{Gly3P}$	0.51^[1]	mM
$Km_{DHAP}$	1.6^[1]	mM

↑ ^1.0 ^1.1 ^1.2 ^1.3 Marín-Hernández A, Gallardo-Pérez JC, Rodríguez-Enríquez S et al (2011) Modeling cancer glycolysis. Biochim Biophys Acta 1807:755–767 (doi) Cite error: Invalid <ref> tag; name "Hernandez2011" defined multiple times with different content
↑ Marín-Hernández A , Rodríguez-Enríquez S, Vital-González P A, et al. (2006). Determining and understanding the control of glycolysis in fast-growth tumor cells. Flux control by an over-expressed but strongly product-inhibited hexokinase. FEBS J., 273 , pp. 1975–1988(doi)

@@ Line 18: / Line 18: @@
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 |<math>V_{mf}</math>
-|325 <ref name="Hernandez2011"> Marín-Hernández A, Gallardo-Pérez JC, Rodríguez-Enríquez S et al (2011). ''Modeling cancer glycolysis''. Biochim Biophys Acta, 1807:755–767 ([http://dx.doi.org/10.1016/j.bbabio.2010.11.006 doi])  </ref>
+|3.4 <ref name="Hernandez2011"> Marín-Hernández A, Gallardo-Pérez JC, Rodríguez-Enríquez S et al (2011). ''Modeling cancer glycolysis''. Biochim Biophys Acta, 1807:755–767 ([http://dx.doi.org/10.1016/j.bbabio.2010.11.006 doi])  </ref>
 |<math> mM \times min^{-1} </math>
 |rowspan="4"|Hela cell line
@@ Line 24: / Line 24: @@
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 |<math>V_{mr}</math>
-|2730<ref name="Hernandez_2006"> Marín-Hernández A , Rodríguez-Enríquez S, Vital-González P A, ''et al.'' (2006). ''Determining and understanding the control of glycolysis in fast-growth tumor cells. Flux control by an over-expressed but strongly product-inhibited hexokinase''. FEBS J., 273 , pp. 1975–1988([http://dx.doi.org/doi:10.1111/j.1742-4658.2006.05214.x doi]) </ref>
+|28<ref name="Hernandez_2006"> Marín-Hernández A , Rodríguez-Enríquez S, Vital-González P A, ''et al.'' (2006). ''Determining and understanding the control of glycolysis in fast-growth tumor cells. Flux control by an over-expressed but strongly product-inhibited hexokinase''. FEBS J., 273 , pp. 1975–1988([http://dx.doi.org/doi:10.1111/j.1742-4658.2006.05214.x doi]) </ref>
 |<math> mM \times min^{-1} </math>
 |-