Difference between revisions of "Transformation of AA to 15-HPETE"

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== 15-LOX Parameters ==
 
== 15-LOX Parameters ==
 
+
===K<sub>ms</sub>===
 
{|class="wikitable sortable"  
 
{|class="wikitable sortable"  
|+  style="text-align: left;" | Michaelis-Menten Constants
+
|+  style="text-align: left;" | Literature values
 
|-
 
|-
 
! Value
 
! Value
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|}
 
|}
  
 +
{| class="wikitable"
 +
|+  style="text-align: left;" | Description of the 15-LOX Kms distribution
 +
! Mode (mM) !! Confidence Interval !! Location parameter (µ) !! Scale parameter (σ)
 +
|-
 +
| 1.02E-02 || 2.40E+00 || -4.42E+00 || 4.10E-01
 +
|-
 +
|
 +
|}
 +
 +
 +
===K<sub>mp</sub>===
 +
{| class="wikitable"
 +
|+  style="text-align: left;" | Description of the 15-LOX Kmp distribution
 +
! Mode (mM) !! Location parameter (µ) !! Scale parameter (σ)
 +
|-
 +
| 1.02E-02 || -4.42E+00 || 4.11E-01
 +
|}
 +
 +
 +
===k<sub>cat</sub>===
 
{|class="wikitable sortable"  
 
{|class="wikitable sortable"  
|+  style="text-align: left;" | Enzyme Turnover Numbers
+
|+  style="text-align: left;" | Literature values
 
|-
 
|-
 
! Value
 
! Value
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|}
 
|}
  
 +
{| class="wikitable"
 +
|+  style="text-align: left;" | Description of the 15-LOX kcat distribution
 +
! Mode (min-1) !! Confidence Interval !! Location parameter (µ) !! Scale parameter (σ)
 +
|-
 +
| 6.51E+01 || 4.62E+00 || 4.60E+00 || 6.50E-01
 +
|}
 +
 +
===Enzyme concentration ===
 
{|class="wikitable sortable"  
 
{|class="wikitable sortable"  
|+  style="text-align: left;" | 15-LOX Abundance
+
|+  style="text-align: left;" | Literature values
 
|-
 
|-
 
! Value
 
! Value
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|}
 
|}
  
 +
{| class="wikitable"
 +
|+  style="text-align: left;" | Description of the 15-LOX concentration distribution
 +
! Mode (mM) !! Confidence Interval !! Location parameter (µ) !! Scale parameter (σ)
 +
|-
 +
| 4.98E-01 || 7.23E+00 || 7.12E-01 || 1.19E+00
 +
|}
 +
 +
===K<sub>eq</sub>===
 
{|class="wikitable sortable"  
 
{|class="wikitable sortable"  
|+  style="text-align: left;" | Gibbs Free Energy Change
+
|+  style="text-align: left;" | Literature values
 
|-
 
|-
! Value
+
! Gibbs Free Energy Change
 
! Units
 
! Units
 
! Species
 
! Species
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|<ref name="MetaCyc”>[http://metacyc.org/META/NEW-IMAGE?type=REACTION&object=RXN66-490 Caspi et al 2014, "The MetaCyc database of metabolic pathways and enzymes and the BioCyc collection of Pathway/Genome Databases," Nucleic Acids Research 42:D459-D471]</ref>
 
|<ref name="MetaCyc”>[http://metacyc.org/META/NEW-IMAGE?type=REACTION&object=RXN66-490 Caspi et al 2014, "The MetaCyc database of metabolic pathways and enzymes and the BioCyc collection of Pathway/Genome Databases," Nucleic Acids Research 42:D459-D471]</ref>
 
|}
 
|}
 +
 +
{| class="wikitable"
 +
|+  style="text-align: left;" | Description of the 15-LOX Keq distribution
 +
! Mode !! Confidence Interval !! Location parameter (µ) !! Scale parameter (σ)
 +
|-
 +
| 2.27E+51 || 1.00E+01 || 1.19E+02 || 8.90E-01
 +
|}
 +
  
 
== References ==
 
== References ==

Revision as of 13:15, 15 May 2019

Return to overview

An isoform homologous to 12-LOX is the 15-LOX enzyme, which produces both 12 and 15-HETE products (Kuhn and O'Donnell 2006). An area of interest is that the 12 and 15 LOX products of the epidermis and the dermis have reciprocal activity during inflammation, for example 15-HETE reduces the infiltration of inflammatory cells, meanwhile 12-HETE acts as an activator for infiltrating immune cells (Dowd, Kobza Black et al. 1985, Serhan, Jain et al. 2003).

Reaction

R17 AA - HPETE15.jpg

Chemical equation

 AA \rightleftharpoons 15-HPETE

Rate equation

R17.PNG

15-LOX Parameters

Kms

Literature values
Value Units Species Notes Reference
3.70E-03 ± 3.00E-04  mM Human Expression Vector: Epithelium

Enzyme: 15-Lipoxygenase pH: 7.5 Temperature: 25

[1]
5.00E-03  mM Human Expression Vector: Reticulocyte

Enzyme: 15-Lipoxygenase pH:7.5 Temperature: Unspecified

[2]
1.06E-02  mM Human Expression Vector: Keratinocyte

Enzyme: 15-Lipoxygenase pH: 6.7 - 7.3 Temperature: 37

[3]
1.17E-02 ± 9.00E-04  mM Human Expression Vector: Baculovirus Insect Cell

Enzyme: Wildtype 15-Lipoxygenase pH: 6.8 Temperature: 37

[4]
Description of the 15-LOX Kms distribution
Mode (mM) Confidence Interval Location parameter (µ) Scale parameter (σ)
1.02E-02 2.40E+00 -4.42E+00 4.10E-01


Kmp

Description of the 15-LOX Kmp distribution
Mode (mM) Location parameter (µ) Scale parameter (σ)
1.02E-02 -4.42E+00 4.11E-01


kcat

Literature values
Value Units Species Notes Reference
595.8 ± 16.8  minute^{-1} Human Expression Vector: Epithelium

Enzyme: 15-Lipoxygenase pH: 7.5 Temperature: 25

[1]
37.2 ± 1.8 per minute Expression Vector:Human prostate epithelial

Enzyme:15-lipoxygenase-2

pH 7, Temperature: 22 [5]
45 ± 1.2 pH 7.5, Temperature: 22
44.4 ± 2.4 pH 8, Temperature: 22
34.2 ± 0.6 15°C, pH = 7.5
45 ± 1.2 22°C, pH = 7.5
62.4 ± 4.2 30°C, pH = 7.5
82.8 ± 4.8 37°C, pH = 7.5
Description of the 15-LOX kcat distribution
Mode (min-1) Confidence Interval Location parameter (µ) Scale parameter (σ)
6.51E+01 4.62E+00 4.60E+00 6.50E-01

Enzyme concentration

Literature values
Value Units Species Notes Reference
4.09  ppm Human Expression Vector: Lung

Enzyme: 15-LOX pH: 7.5 Temperature: 37 °C

[6]
37.4  ppm Human Expression Vector: Spleen

Enzyme: 15-LOX pH: 7.5 Temperature: 37 °C

[7]
1.40  ppm Human Expression Vector: Gut

Enzyme: 12-LOX pH: 7.5 Temperature: 37 °C

[6]
Description of the 15-LOX concentration distribution
Mode (mM) Confidence Interval Location parameter (µ) Scale parameter (σ)
4.98E-01 7.23E+00 7.12E-01 1.19E+00

Keq

Literature values
Gibbs Free Energy Change Units Species Notes Reference
(-69.979996) kcal/mol Not stated Estimated

Enzyme: 15-LOX Substrate: Arachidonate Product: 15-HPETE pH: 7.3 ionic strength: 0.25

[8]
Description of the 15-LOX Keq distribution
Mode Confidence Interval Location parameter (µ) Scale parameter (σ)
2.27E+51 1.00E+01 1.19E+02 8.90E-01


References

Related Reactions