Difference between revisions of "Transformation of 5-HPETE to 5-HETE"
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==Chemical equation== | ==Chemical equation== | ||
− | <center><math> | + | <center><math> 5-HPETE \rightleftharpoons 5-HETE </math></center> |
== Rate equation == | == Rate equation == | ||
+ | [[File:R12.PNG|center|500px]] | ||
== PHGPx Parameters == | == PHGPx Parameters == |
Revision as of 12:02, 26 September 2017
5-HPETE is reduced by phospholipid hydroperoxide glutathione peroxidase (PHGPx) to the corresponding alcohol, 5-HETE (Suth01).
Contents
Reaction
Chemical equation
Rate equation
PHGPx Parameters
Value | Units | Species | Notes | Reference |
---|---|---|---|---|
3.00E-01 | Human | Expression Vector: Bioimprited Enzyme - Selenosubtilisin
Enzyme: Glutathione Peroxidase pH: 7 Temperature: 37 |
[1] | |
11.1 ± 2.90E-01 | Human | Expression Vector: E Coli
Enzyme: Wild Type Glutathione Peroxidase Enzyme (Se-hGSTZ1-1) pH: 7 Temperature: 37 |
[2] | |
4.00E-03 | Rat | Expression Vector: Rat Liver Cells
Enzyme: Glutathione Peroxidase pH: 7.4 Temperature: 37 |
[3] |
Value | Units | Species | Notes | Reference |
---|---|---|---|---|
1170 ± 50 | Human | Expression Vector: Bioimprited Enzyme - Selenosubtilisin
Enzyme: Glutathione Peroxidase pH: 7 Temperature: 37 |
[1] | |
24500 ± 150 | Human | Expression Vector: E Coli
Enzyme: Wild Type Glutathione Peroxidase Enzyme (Se-hGSTZ1-1) pH: 7 Temperature: 37 |
[2] |
Value | Units | Species | Notes | Reference |
---|---|---|---|---|
459 | Human | Expression Vector: Skin
Enzyme: PHGPx pH: 7.5 Temperature: 37 °C |
[4] | |
307 | Human | Expression Vector: Lung
Enzyme: PHGPx pH: 7.5 Temperature: 37 °C |
[4] | |
282 | Human | Expression Vector: Esophagus
Enzyme: PHGPx pH: 7.5 Temperature: 37 °C |
[4] | |
204 | Human | Expression Vector: Gut
Enzyme: PHGPx pH: 7.5 Temperature: 37 °C |
[5] |
Value | Units | Species | Notes | Reference |
---|---|---|---|---|
(-26.941177) | kcal/mol | Not stated | Estimated
Enzyme: PHGPx Substrate:a hydroperoxy-fatty-acyl-[lipid] Product: a hydroxy-fatty-acyl-[lipid] pH: 7.3 ionic strength: 0.25 |
[6] |
References
- ↑ 1.0 1.1 Liu L. "Functional mimicry of the active site of glutathione peroxidase by glutathione imprinted selenium-containing protein. Biomacromolecules. 2008 Jan;9(1):363-8. doi: 10.1021/bm7008312. Epub 2007 Dec 29.
- ↑ 2.0 2.1 Zheng K. "A novel selenium-containing glutathione transferase zeta1-1, the activity of which surpasses the level of some native glutathione peroxidases. Int J Biochem Cell Biol. 2008;40(10):2090-7. doi: 10.1016/j.biocel.2008.02.006. Epub 2008 Feb 15.
- ↑ Hiratsuka A. "Subunit Ya-specific glutathione peroxidase activity toward cholesterol 7-hydroperoxides of glutathione S-transferases in cytosols from rat liver and skin. J Biol Chem. 1997 Feb 21;272(8):4763-9.
- ↑ 4.0 4.1 4.2 M. Wilhelm Mass-spectrometry-based draft of the human proteome Nature, 2014 509, 582–587
- ↑ M. Kim A draft map of the human proteome Nature, 2014 509, 575–581
- ↑ Caspi et al 2014, "The MetaCyc database of metabolic pathways and enzymes and the BioCyc collection of Pathway/Genome Databases," Nucleic Acids Research 42:D459-D471