Transformation of 5-HPETE to 5-HETE

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5-HPETE is reduced by phospholipid hydroperoxide glutathione peroxidase (PHGPx) to the corresponding alcohol, 5-HETE (Suth01).

Reaction

Chemical equation

$5-HPETE \rightleftharpoons 5-HETE$

Rate equation

PHGPx Parameters

Michaelis-Menten Constants

Value	Units	Species	Notes	Reference
3.00E-01	$mM$	Human	Expression Vector: Bioimprited Enzyme - Selenosubtilisin Enzyme: Glutathione Peroxidase pH: 7 Temperature: 37
11.1 ± 2.90E-01	$mM$	Human	Expression Vector: E Coli Enzyme: Wild Type Glutathione Peroxidase Enzyme (Se-hGSTZ1-1) pH: 7 Temperature: 37
4.00E-03	$mM$	Rat	Expression Vector: Rat Liver Cells Enzyme: Glutathione Peroxidase pH: 7.4 Temperature: 37

Enzyme Turnover Numbers

Value	Units	Species	Notes	Reference
1170 ± 50	$min^{-1}$	Human	Expression Vector: Bioimprited Enzyme - Selenosubtilisin Enzyme: Glutathione Peroxidase pH: 7 Temperature: 37
24500 ± 150	$min^{-1}$	Human	Expression Vector: E Coli Enzyme: Wild Type Glutathione Peroxidase Enzyme (Se-hGSTZ1-1) pH: 7 Temperature: 37

PHGPx Abundance

Value	Units	Species	Notes	Reference
459	$ppm$	Human	Expression Vector: Skin Enzyme: PHGPx pH: 7.5 Temperature: 37 °C
307	$ppm$	Human	Expression Vector: Lung Enzyme: PHGPx pH: 7.5 Temperature: 37 °C
282	$ppm$	Human	Expression Vector: Esophagus Enzyme: PHGPx pH: 7.5 Temperature: 37 °C
204	$ppm$	Human	Expression Vector: Gut Enzyme: PHGPx pH: 7.5 Temperature: 37 °C

Gibbs Free Energy Change

Value	Units	Species	Notes	Reference
(-26.941177)	kcal/mol	Not stated	Estimated Enzyme: PHGPx Substrate:a hydroperoxy-fatty-acyl-[lipid] Product: a hydroxy-fatty-acyl-[lipid] pH: 7.3 ionic strength: 0.25