Difference between revisions of "Transformation of 5-HPETE to 5-HETE"
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|<math> mM </math> | |<math> mM </math> | ||
|Human | |Human | ||
− | |Bioimprited Enzyme | + | |Expression Vector: Bioimprited Enzyme - Selenosubtilisin |
+ | Enzyme: Glutathione Peroxidase | ||
+ | pH: 7 | ||
+ | Temperature: 37 | ||
|<ref name="Liu2008"> [http://www.ncbi.nlm.nih.gov/pubmed/18163571 Liu L. "Functional mimicry of the active site of glutathione peroxidase by glutathione imprinted selenium-containing protein.'' Biomacromolecules. 2008 Jan;9(1):363-8. doi: 10.1021/bm7008312. Epub 2007 Dec 29.]</ref> | |<ref name="Liu2008"> [http://www.ncbi.nlm.nih.gov/pubmed/18163571 Liu L. "Functional mimicry of the active site of glutathione peroxidase by glutathione imprinted selenium-containing protein.'' Biomacromolecules. 2008 Jan;9(1):363-8. doi: 10.1021/bm7008312. Epub 2007 Dec 29.]</ref> | ||
|- | |- | ||
Line 31: | Line 34: | ||
|<math> mM </math> | |<math> mM </math> | ||
|Human | |Human | ||
− | |Wild Type Enzyme (Se-hGSTZ1-1) | + | |Expression Vector: E Coli |
+ | Enzyme: Wild Type Glutathione Peroxidase Enzyme (Se-hGSTZ1-1) | ||
+ | pH: 7 | ||
+ | Temperature: 37 | ||
|<ref name="Zheng2008"> [http://www.ncbi.nlm.nih.gov/pubmed/18373941 Zheng K. "A novel selenium-containing glutathione transferase zeta1-1, the activity of which surpasses the level of some native glutathione peroxidases.'' Int J Biochem Cell Biol. 2008;40(10):2090-7. doi: 10.1016/j.biocel.2008.02.006. Epub 2008 Feb 15.]</ref> | |<ref name="Zheng2008"> [http://www.ncbi.nlm.nih.gov/pubmed/18373941 Zheng K. "A novel selenium-containing glutathione transferase zeta1-1, the activity of which surpasses the level of some native glutathione peroxidases.'' Int J Biochem Cell Biol. 2008;40(10):2090-7. doi: 10.1016/j.biocel.2008.02.006. Epub 2008 Feb 15.]</ref> | ||
|- | |- | ||
Line 37: | Line 43: | ||
|<math> mM </math> | |<math> mM </math> | ||
|Rat | |Rat | ||
− | |Liver | + | |Expression Vector: Rat Liver Cells |
+ | Enzyme: Glutathione Peroxidase | ||
+ | pH: 7.4 | ||
+ | Temperature: 37 | ||
+ | |||
|<ref name="Hirat1997"> [http://www.ncbi.nlm.nih.gov/pubmed/9030530 Hiratsuka A. "Subunit Ya-specific glutathione peroxidase activity toward cholesterol 7-hydroperoxides of glutathione S-transferases in cytosols from rat liver and skin.'' J Biol Chem. 1997 Feb 21;272(8):4763-9.]</ref> | |<ref name="Hirat1997"> [http://www.ncbi.nlm.nih.gov/pubmed/9030530 Hiratsuka A. "Subunit Ya-specific glutathione peroxidase activity toward cholesterol 7-hydroperoxides of glutathione S-transferases in cytosols from rat liver and skin.'' J Biol Chem. 1997 Feb 21;272(8):4763-9.]</ref> | ||
|- | |- | ||
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|<math> min^{-1} </math> | |<math> min^{-1} </math> | ||
|Human | |Human | ||
− | |Bioimprited Enzyme | + | |Expression Vector: Bioimprited Enzyme - Selenosubtilisin |
+ | Enzyme: Glutathione Peroxidase | ||
+ | pH: 7 | ||
+ | Temperature: 37 | ||
|<ref name="Liu2008"> [http://www.ncbi.nlm.nih.gov/pubmed/18163571 Liu L. "Functional mimicry of the active site of glutathione peroxidase by glutathione imprinted selenium-containing protein.'' Biomacromolecules. 2008 Jan;9(1):363-8. doi: 10.1021/bm7008312. Epub 2007 Dec 29.]</ref> | |<ref name="Liu2008"> [http://www.ncbi.nlm.nih.gov/pubmed/18163571 Liu L. "Functional mimicry of the active site of glutathione peroxidase by glutathione imprinted selenium-containing protein.'' Biomacromolecules. 2008 Jan;9(1):363-8. doi: 10.1021/bm7008312. Epub 2007 Dec 29.]</ref> | ||
|- | |- | ||
Line 60: | Line 73: | ||
|<math> min^{-1} </math> | |<math> min^{-1} </math> | ||
|Human | |Human | ||
− | |Wild Type Enzyme (Se-hGSTZ1-1) | + | |Expression Vector: E Coli |
+ | Enzyme: Wild Type Glutathione Peroxidase Enzyme (Se-hGSTZ1-1) | ||
+ | pH: 7 | ||
+ | Temperature: 37 | ||
|<ref name="Zheng2008"> [http://www.ncbi.nlm.nih.gov/pubmed/18373941 Zheng K. "A novel selenium-containing glutathione transferase zeta1-1, the activity of which surpasses the level of some native glutathione peroxidases.'' Int J Biochem Cell Biol. 2008;40(10):2090-7. doi: 10.1016/j.biocel.2008.02.006. Epub 2008 Feb 15.]</ref> | |<ref name="Zheng2008"> [http://www.ncbi.nlm.nih.gov/pubmed/18373941 Zheng K. "A novel selenium-containing glutathione transferase zeta1-1, the activity of which surpasses the level of some native glutathione peroxidases.'' Int J Biochem Cell Biol. 2008;40(10):2090-7. doi: 10.1016/j.biocel.2008.02.006. Epub 2008 Feb 15.]</ref> | ||
|- | |- | ||
Line 77: | Line 93: | ||
|mM | |mM | ||
|Human | |Human | ||
− | |Bioimprited Enzyme | + | |Expression Vector: Bioimprited Enzyme - Selenosubtilisin |
+ | Enzyme: Glutathione Peroxidase | ||
+ | pH: 7 | ||
+ | Temperature: 37 | ||
|<ref name="Liu2008"> [http://www.ncbi.nlm.nih.gov/pubmed/18163571 Liu L. "Functional mimicry of the active site of glutathione peroxidase by glutathione imprinted selenium-containing protein.'' Biomacromolecules. 2008 Jan;9(1):363-8. doi: 10.1021/bm7008312. Epub 2007 Dec 29.]</ref> | |<ref name="Liu2008"> [http://www.ncbi.nlm.nih.gov/pubmed/18163571 Liu L. "Functional mimicry of the active site of glutathione peroxidase by glutathione imprinted selenium-containing protein.'' Biomacromolecules. 2008 Jan;9(1):363-8. doi: 10.1021/bm7008312. Epub 2007 Dec 29.]</ref> | ||
|- | |- | ||
Line 83: | Line 102: | ||
|mM | |mM | ||
|Human | |Human | ||
− | |Wild Type Enzyme (Se-hGSTZ1-1) | + | |Expression Vector: E Coli |
+ | Enzyme: Wild Type Glutathione Peroxidase Enzyme (Se-hGSTZ1-1) | ||
+ | pH: 7 | ||
+ | Temperature: 37 | ||
|<ref name="Zheng2008"> [http://www.ncbi.nlm.nih.gov/pubmed/18373941 Zheng K. "A novel selenium-containing glutathione transferase zeta1-1, the activity of which surpasses the level of some native glutathione peroxidases.'' Int J Biochem Cell Biol. 2008;40(10):2090-7. doi: 10.1016/j.biocel.2008.02.006. Epub 2008 Feb 15.]</ref> | |<ref name="Zheng2008"> [http://www.ncbi.nlm.nih.gov/pubmed/18373941 Zheng K. "A novel selenium-containing glutathione transferase zeta1-1, the activity of which surpasses the level of some native glutathione peroxidases.'' Int J Biochem Cell Biol. 2008;40(10):2090-7. doi: 10.1016/j.biocel.2008.02.006. Epub 2008 Feb 15.]</ref> | ||
|} | |} |
Revision as of 11:01, 9 June 2016
Contents
Reaction
Chemical equation
Rate equation
PHGPx Parameters
Value | Units | Species | Notes | Reference |
---|---|---|---|---|
3.00E-01 | Human | Expression Vector: Bioimprited Enzyme - Selenosubtilisin
Enzyme: Glutathione Peroxidase pH: 7 Temperature: 37 |
[1] | |
11.1 ± 2.90E-01 | Human | Expression Vector: E Coli
Enzyme: Wild Type Glutathione Peroxidase Enzyme (Se-hGSTZ1-1) pH: 7 Temperature: 37 |
[2] | |
4.00E-03 | Rat | Expression Vector: Rat Liver Cells
Enzyme: Glutathione Peroxidase pH: 7.4 Temperature: 37 |
[3] |
Value | Units | Species | Notes | Reference |
---|---|---|---|---|
1170 ± 50 | Human | Expression Vector: Bioimprited Enzyme - Selenosubtilisin
Enzyme: Glutathione Peroxidase pH: 7 Temperature: 37 |
[1] | |
24500 ± 150 | Human | Expression Vector: E Coli
Enzyme: Wild Type Glutathione Peroxidase Enzyme (Se-hGSTZ1-1) pH: 7 Temperature: 37 |
[2] |
Value | Units | Species | Notes | Reference |
---|---|---|---|---|
0.0005-0.005 | mM | Human | Expression Vector: Bioimprited Enzyme - Selenosubtilisin
Enzyme: Glutathione Peroxidase pH: 7 Temperature: 37 |
[1] |
0.004 | mM | Human | Expression Vector: E Coli
Enzyme: Wild Type Glutathione Peroxidase Enzyme (Se-hGSTZ1-1) pH: 7 Temperature: 37 |
[2] |
References
- ↑ 1.0 1.1 1.2 Liu L. "Functional mimicry of the active site of glutathione peroxidase by glutathione imprinted selenium-containing protein. Biomacromolecules. 2008 Jan;9(1):363-8. doi: 10.1021/bm7008312. Epub 2007 Dec 29.
- ↑ 2.0 2.1 2.2 Zheng K. "A novel selenium-containing glutathione transferase zeta1-1, the activity of which surpasses the level of some native glutathione peroxidases. Int J Biochem Cell Biol. 2008;40(10):2090-7. doi: 10.1016/j.biocel.2008.02.006. Epub 2008 Feb 15.
- ↑ Hiratsuka A. "Subunit Ya-specific glutathione peroxidase activity toward cholesterol 7-hydroperoxides of glutathione S-transferases in cytosols from rat liver and skin. J Biol Chem. 1997 Feb 21;272(8):4763-9.