Difference between revisions of "Transformation of 5-HPETE to 5-HETE"

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(PHGPx Parameters)
(PHGPx Parameters)
Line 25: Line 25:
 
|<math> mM </math>
 
|<math> mM </math>
 
|Human  
 
|Human  
|Bioimprited Enzyme  
+
|Expression Vector: Bioimprited Enzyme - Selenosubtilisin
 +
Enzyme: Glutathione Peroxidase
 +
pH: 7
 +
Temperature: 37
 
|<ref name="Liu2008"> [http://www.ncbi.nlm.nih.gov/pubmed/18163571 Liu L. "Functional mimicry of the active site of glutathione peroxidase by glutathione imprinted selenium-containing protein.'' Biomacromolecules. 2008 Jan;9(1):363-8. doi: 10.1021/bm7008312. Epub 2007 Dec 29.]</ref>  
 
|<ref name="Liu2008"> [http://www.ncbi.nlm.nih.gov/pubmed/18163571 Liu L. "Functional mimicry of the active site of glutathione peroxidase by glutathione imprinted selenium-containing protein.'' Biomacromolecules. 2008 Jan;9(1):363-8. doi: 10.1021/bm7008312. Epub 2007 Dec 29.]</ref>  
 
|-
 
|-
Line 31: Line 34:
 
|<math> mM </math>
 
|<math> mM </math>
 
|Human  
 
|Human  
|Wild Type Enzyme (Se-hGSTZ1-1)
+
|Expression Vector: E Coli
 +
Enzyme: Wild Type Glutathione Peroxidase Enzyme (Se-hGSTZ1-1)
 +
pH: 7
 +
Temperature: 37
 
|<ref name="Zheng2008"> [http://www.ncbi.nlm.nih.gov/pubmed/18373941 Zheng K. "A novel selenium-containing glutathione transferase zeta1-1, the activity of which surpasses the level of some native glutathione peroxidases.'' Int J Biochem Cell Biol. 2008;40(10):2090-7. doi: 10.1016/j.biocel.2008.02.006. Epub 2008 Feb 15.]</ref>  
 
|<ref name="Zheng2008"> [http://www.ncbi.nlm.nih.gov/pubmed/18373941 Zheng K. "A novel selenium-containing glutathione transferase zeta1-1, the activity of which surpasses the level of some native glutathione peroxidases.'' Int J Biochem Cell Biol. 2008;40(10):2090-7. doi: 10.1016/j.biocel.2008.02.006. Epub 2008 Feb 15.]</ref>  
 
|-
 
|-
Line 37: Line 43:
 
|<math> mM </math>
 
|<math> mM </math>
 
|Rat  
 
|Rat  
|Liver cytosol Se-GSH Px (5-HPETE as the substrate)
+
|Expression Vector: Rat Liver Cells
 +
Enzyme: Glutathione Peroxidase
 +
pH: 7.4
 +
Temperature: 37
 +
 
 
|<ref name="Hirat1997"> [http://www.ncbi.nlm.nih.gov/pubmed/9030530 Hiratsuka A. "Subunit Ya-specific glutathione peroxidase activity toward cholesterol 7-hydroperoxides of glutathione S-transferases in cytosols from rat liver and skin.'' J Biol Chem. 1997 Feb 21;272(8):4763-9.]</ref>  
 
|<ref name="Hirat1997"> [http://www.ncbi.nlm.nih.gov/pubmed/9030530 Hiratsuka A. "Subunit Ya-specific glutathione peroxidase activity toward cholesterol 7-hydroperoxides of glutathione S-transferases in cytosols from rat liver and skin.'' J Biol Chem. 1997 Feb 21;272(8):4763-9.]</ref>  
 
|-
 
|-
Line 54: Line 64:
 
|<math> min^{-1} </math>
 
|<math> min^{-1} </math>
 
|Human  
 
|Human  
|Bioimprited Enzyme  
+
|Expression Vector: Bioimprited Enzyme - Selenosubtilisin
 +
Enzyme: Glutathione Peroxidase
 +
pH: 7
 +
Temperature: 37
 
|<ref name="Liu2008"> [http://www.ncbi.nlm.nih.gov/pubmed/18163571 Liu L. "Functional mimicry of the active site of glutathione peroxidase by glutathione imprinted selenium-containing protein.'' Biomacromolecules. 2008 Jan;9(1):363-8. doi: 10.1021/bm7008312. Epub 2007 Dec 29.]</ref>  
 
|<ref name="Liu2008"> [http://www.ncbi.nlm.nih.gov/pubmed/18163571 Liu L. "Functional mimicry of the active site of glutathione peroxidase by glutathione imprinted selenium-containing protein.'' Biomacromolecules. 2008 Jan;9(1):363-8. doi: 10.1021/bm7008312. Epub 2007 Dec 29.]</ref>  
 
|-
 
|-
Line 60: Line 73:
 
|<math> min^{-1} </math>
 
|<math> min^{-1} </math>
 
|Human  
 
|Human  
|Wild Type Enzyme (Se-hGSTZ1-1)
+
|Expression Vector: E Coli
 +
Enzyme: Wild Type Glutathione Peroxidase Enzyme (Se-hGSTZ1-1)
 +
pH: 7
 +
Temperature: 37
 
|<ref name="Zheng2008"> [http://www.ncbi.nlm.nih.gov/pubmed/18373941 Zheng K. "A novel selenium-containing glutathione transferase zeta1-1, the activity of which surpasses the level of some native glutathione peroxidases.'' Int J Biochem Cell Biol. 2008;40(10):2090-7. doi: 10.1016/j.biocel.2008.02.006. Epub 2008 Feb 15.]</ref>  
 
|<ref name="Zheng2008"> [http://www.ncbi.nlm.nih.gov/pubmed/18373941 Zheng K. "A novel selenium-containing glutathione transferase zeta1-1, the activity of which surpasses the level of some native glutathione peroxidases.'' Int J Biochem Cell Biol. 2008;40(10):2090-7. doi: 10.1016/j.biocel.2008.02.006. Epub 2008 Feb 15.]</ref>  
 
|-
 
|-
Line 77: Line 93:
 
|mM
 
|mM
 
|Human  
 
|Human  
|Bioimprited Enzyme  
+
|Expression Vector: Bioimprited Enzyme - Selenosubtilisin
 +
Enzyme: Glutathione Peroxidase
 +
pH: 7
 +
Temperature: 37
 
|<ref name="Liu2008"> [http://www.ncbi.nlm.nih.gov/pubmed/18163571 Liu L. "Functional mimicry of the active site of glutathione peroxidase by glutathione imprinted selenium-containing protein.'' Biomacromolecules. 2008 Jan;9(1):363-8. doi: 10.1021/bm7008312. Epub 2007 Dec 29.]</ref>
 
|<ref name="Liu2008"> [http://www.ncbi.nlm.nih.gov/pubmed/18163571 Liu L. "Functional mimicry of the active site of glutathione peroxidase by glutathione imprinted selenium-containing protein.'' Biomacromolecules. 2008 Jan;9(1):363-8. doi: 10.1021/bm7008312. Epub 2007 Dec 29.]</ref>
 
|-
 
|-
Line 83: Line 102:
 
|mM
 
|mM
 
|Human  
 
|Human  
|Wild Type Enzyme (Se-hGSTZ1-1)
+
|Expression Vector: E Coli
 +
Enzyme: Wild Type Glutathione Peroxidase Enzyme (Se-hGSTZ1-1)
 +
pH: 7
 +
Temperature: 37
 
|<ref name="Zheng2008"> [http://www.ncbi.nlm.nih.gov/pubmed/18373941 Zheng K. "A novel selenium-containing glutathione transferase zeta1-1, the activity of which surpasses the level of some native glutathione peroxidases.'' Int J Biochem Cell Biol. 2008;40(10):2090-7. doi: 10.1016/j.biocel.2008.02.006. Epub 2008 Feb 15.]</ref>  
 
|<ref name="Zheng2008"> [http://www.ncbi.nlm.nih.gov/pubmed/18373941 Zheng K. "A novel selenium-containing glutathione transferase zeta1-1, the activity of which surpasses the level of some native glutathione peroxidases.'' Int J Biochem Cell Biol. 2008;40(10):2090-7. doi: 10.1016/j.biocel.2008.02.006. Epub 2008 Feb 15.]</ref>  
 
|}
 
|}

Revision as of 11:01, 9 June 2016

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R12 HPETE5-HETE5.jpg

Chemical equation

 AA \rightleftharpoons PGH2

Rate equation

PHGPx Parameters

Michaelis-Menten Constants
Value Units Species Notes Reference
3.00E-01  mM Human Expression Vector: Bioimprited Enzyme - Selenosubtilisin

Enzyme: Glutathione Peroxidase pH: 7 Temperature: 37

[1]
11.1 ± 2.90E-01  mM Human Expression Vector: E Coli

Enzyme: Wild Type Glutathione Peroxidase Enzyme (Se-hGSTZ1-1) pH: 7 Temperature: 37

[2]
4.00E-03  mM Rat Expression Vector: Rat Liver Cells

Enzyme: Glutathione Peroxidase pH: 7.4 Temperature: 37

[3]
Enzyme Turnover Numbers
Value Units Species Notes Reference
1170 ± 50  min^{-1} Human Expression Vector: Bioimprited Enzyme - Selenosubtilisin

Enzyme: Glutathione Peroxidase pH: 7 Temperature: 37

[1]
24500 ± 150  min^{-1} Human Expression Vector: E Coli

Enzyme: Wild Type Glutathione Peroxidase Enzyme (Se-hGSTZ1-1) pH: 7 Temperature: 37

[2]
Enzyme Concentrations
Value Units Species Notes Reference
0.0005-0.005 mM Human Expression Vector: Bioimprited Enzyme - Selenosubtilisin

Enzyme: Glutathione Peroxidase pH: 7 Temperature: 37

[1]
0.004 mM Human Expression Vector: E Coli

Enzyme: Wild Type Glutathione Peroxidase Enzyme (Se-hGSTZ1-1) pH: 7 Temperature: 37

[2]

References

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