Difference between revisions of "Fructose 1,6 bisphosphate aldolase"
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|<math>V_{mf}</math> | |<math>V_{mf}</math> | ||
− | |<math>0.2 \pm 0.05 (5) </math> <ref name="Hernandez_2006"> Marín-Hernández A , Rodríguez-Enríquez S, Vital-González P A, ''et al.'' (2006). ''Determining and understanding the control of glycolysis in fast-growth tumor cells. Flux control by an over-expressed but strongly product-inhibited hexokinase''. FEBS J., 273 , pp. 1975–1988([http://dx.doi.org/doi:10.1111/j.1742-4658.2006.05214.x doi]) </ref> | + | |<math>0.2 \pm 0.05 (5) </math> <ref name="Hernandez_2006"> Marín-Hernández A , Rodríguez-Enríquez S, Vital-González P A, ''et al.'' (2006). ''Determining and understanding the control of glycolysis in fast-growth tumor cells. Flux control by an over-expressed but strongly product-inhibited hexokinase''. FEBS J., 273 , pp. 1975–1988([http://dx.doi.org/doi:10.1111/j.1742-4658.2006.05214.x doi]) </ref> <br> <math> 13 \pm 3.25</math> |
− | |<math> mM \times min^{-1} </math> | + | |<math>U\cdot(\text{mg protein})^{-1}</math><br><math> mM \times min^{-1} </math> |
|Hela cell line | |Hela cell line | ||
|rowspan="5"| | |rowspan="5"| | ||
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|<math>V_{mr}</math> | |<math>V_{mr}</math> | ||
|Sampled based on Haldane equation. <br> '''Alternative:'''<math>0.063 \pm 0.028 </math> | |Sampled based on Haldane equation. <br> '''Alternative:'''<math>0.063 \pm 0.028 </math> | ||
− | |<math> | + | |<math>U\cdot(\text{mg protein})^{-1}</math> |
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| pH=7, T=25°C | | pH=7, T=25°C | ||
| Voet et al.<ref name="voet">Voet, D., Voet., J.G. and Pratt, C. W. (1999) Fundamentals of biochemistry, Wiley</ref> from Newshole et al. (1973) <ref name="newshole73">Newshole, E.A. and Stuart, C. (1973) Regulation in Metabolism, Wiley</ref>p 97:<br/> | | Voet et al.<ref name="voet">Voet, D., Voet., J.G. and Pratt, C. W. (1999) Fundamentals of biochemistry, Wiley</ref> from Newshole et al. (1973) <ref name="newshole73">Newshole, E.A. and Stuart, C. (1973) Regulation in Metabolism, Wiley</ref>p 97:<br/> | ||
− | <math>\Delta G' = 22.8\ kJ.mol^{-1}</math>, <math>Keq = exp(-\frac{\Delta G'}{RT}) = exp(\frac{-22800}{8.31*298.15}) \approx 0.10</math> | + | <math>\Delta G' = 22.8\ kJ.mol^{-1}</math>, <math>Keq = exp(-\frac{\Delta G'}{RT}) = exp(\frac{-22800}{8.31*298.15}) \approx 0.10</math> mM (As there are one substrate and two product necessary conversion in units are to be made). |
|- | |- | ||
| 0.067 | | 0.067 | ||
| pH=7, T=25°C | | pH=7, T=25°C | ||
| Lehninger, (1975)<ref name="lehninger75">Lehninger, A.L. (1975) Biochemistry (2nd edn), Worth</ref> p 407:<br/> | | Lehninger, (1975)<ref name="lehninger75">Lehninger, A.L. (1975) Biochemistry (2nd edn), Worth</ref> p 407:<br/> | ||
− | <math>\Delta G' = 23.8\ kJ.mol^{-1}</math>, <math>Keq = exp(-\frac{\Delta G'}{RT}) = exp(\frac{-23800}{8.31*298.15}) \approx 0.067</math> | + | <math>\Delta G' = 23.8\ kJ.mol^{-1}</math>, <math>Keq = exp(-\frac{\Delta G'}{RT}) = exp(\frac{-23800}{8.31*298.15}) \approx 0.067</math> mM (As there are one substrate and two product necessary conversion in units are to be made). |
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Latest revision as of 10:46, 16 October 2014
This enzyme splits fructose 1, 6-bisphosphate into two sugars that are isomers of each other. These two sugars are Dihydroxyacetone phosphate (DHAP) and Glyceraldehyde 3-phosphate (Gly3P).
Contents
Chemical equation
![Fru1,6BP \rightleftharpoons Gly3P + DHAP](/wiki/images/math/8/1/0/8109d3b19111514e7b52001dd5ea1219.png)
Rate equation
Reversible Uni-Bi Michaelis-Menten is used. [1]
![\frac{V_{mf} \frac{[Fru1,6BP]}{K_{Fru1,6BP}} - V_{mr}\frac{[DHAP][G3P]}{K_{DHAP}K_{Gly3P}} }{1 + \frac{[Fru1,6BP]}{K_{Fru1,6BP}} + \frac{[DHAP]}{K_{DHAP}} +\frac{[Gly3P]}{K_{Gly3P}} + \frac{[DHAP][Gly3P]}{K_{DHAP}K_{Gly3P}} }](/wiki/images/math/d/f/0/df0be717a9e6f06e44e264eacb312fd7.png)
Modified rate law to take into consideration the equilibrium constant
![\frac{V_{mf} \frac{[Fru1,6BP]}{K_{Fru1,6BP}} \left(1- \frac{[DHAP][Gly3P]}{K_{eq}[Fru1,6BP]} \right)}{1 + \frac{[Fru1,6BP]}{K_{Fru1,6BP}} + \frac{[DHAP]}{K_{DHAP}} +\frac{[Gly3P]}{K_{Gly3P}} + \frac{[DHAP][Gly3P]}{K_{DHAP}K_{Gly3P}} }](/wiki/images/math/7/d/a/7da8fdee0d5e4c1d0182c19cda3d9a69.png)
Parameters
Parameter | Value | Units | Organism | Remarks |
---|---|---|---|---|
![]() |
0.08 [2] | ![]() |
Hela cell line | |
![]() |
0.063[2] | ![]() |
Rodent AS-30D hepatoma | |
![]() |
0.009[2] | mM | Hela cell line | |
![]() |
0.16[2] | mM | Rodent AS-30D hepatoma | |
![]() |
0.08[2] | mM | Rodent AS-30D hepatoma |
Parameters with uncertainty
- The value for
is collected from Hernandez et. al. [2]. The
is calcualted from the sampled
,
,
and
values using the Haldane equation. Alternatively the reported value from AS-30D can be considered. The standard deviation can be calculated based on the same ratio of
. However, as it is from a different species an extra 20% of uncertainty will be added. This gives the value
- The value for
are collected from Ali D. Malay et. al. [3] for wildtype Aldolase B gene at
C.
Parameter | Value | Units | Organism | Remarks |
---|---|---|---|---|
![]() |
![]() ![]() |
![]() ![]() |
Hela cell line | |
![]() |
Sampled based on Haldane equation. Alternative: ![]() |
![]() |
||
![]() |
![]() |
mM | Human cell | |
![]() |
![]() |
mM | Human cell | |
![]() |
![]() |
mM | Human cell |
Equilibrium constant
Equilibrium constant | Conditions | Source |
---|---|---|
0.10 | pH=7, T=25°C | Voet et al.[4] from Newshole et al. (1973) [5]p 97:
|
0.067 | pH=7, T=25°C | Lehninger, (1975)[6] p 407:
|
- Taking average of these two values give
References
- ↑ Marín-Hernández A, Gallardo-Pérez JC, Rodríguez-Enríquez S et al (2011) Modeling cancer glycolysis. Biochim Biophys Acta 1807:755–767 (doi)
- ↑ 2.0 2.1 2.2 2.3 2.4 2.5 2.6 Marín-Hernández A , Rodríguez-Enríquez S, Vital-González P A, et al. (2006). Determining and understanding the control of glycolysis in fast-growth tumor cells. Flux control by an over-expressed but strongly product-inhibited hexokinase. FEBS J., 273 , pp. 1975–1988(doi)
- ↑ Malay AD, Procious SL, Tolan DR. (2002). The temperature dependence of activity and structure for most prevalent mutant aldolase B associated with hereditary fructose intolerance, Arch BiochemBiophys 408: 295–304.
- ↑ Voet, D., Voet., J.G. and Pratt, C. W. (1999) Fundamentals of biochemistry, Wiley
- ↑ Newshole, E.A. and Stuart, C. (1973) Regulation in Metabolism, Wiley
- ↑ Lehninger, A.L. (1975) Biochemistry (2nd edn), Worth