Difference between revisions of "46BR.1N fibroblasts Fatty acid analysis Results"

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[[Welcome to the In-Silico Model of Cutaneous Lipids Wiki | Return to overview]]
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[[46BR.1N Fibroblasts|Return to 46BR.1N Fibroblasts]]
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The aim of this study was to estimate the levels of AA in cells which were unstimulated and stimulated with A23187 (with/without COX inhibition), ATP and UVR. GC-FID was used for this fatty acid analysis and the values were normalized using cell numbers (ug/106 cells).
  
== Summary Table ==
 
 
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{| class="wikitable"
 
|+  style="text-align: left;" |The estimated AA levels in 46BR.1N fibroblasts (unstimulated, A23187 (5µM), COX inhibited and A23187 (5 µM), ATP (2 mM) and UVR (15 mJ/cm2)) for 0.5, 1, 3 and 6h. Analysis was performed using GC-FID. Results are expressed mean ± SD (ug/106 cells) of three independent experiments.
 
|+  style="text-align: left;" |The estimated AA levels in 46BR.1N fibroblasts (unstimulated, A23187 (5µM), COX inhibited and A23187 (5 µM), ATP (2 mM) and UVR (15 mJ/cm2)) for 0.5, 1, 3 and 6h. Analysis was performed using GC-FID. Results are expressed mean ± SD (ug/106 cells) of three independent experiments.
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| style="text-align: center;" | 1.03 ± 0.22
 
| style="text-align: center;" | 1.03 ± 0.22
 
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[[Image:Firbo AA.jpg|none|thumb|500px|Levels of cellular arachidonic acid in 46BR.1N fibroblasts following stimulation with (A) A23187, (B) UVR and (C) ATP 6h post stimulation.  Data points are from n=3 independent experiments. Non-linear lines of best fit are shown for untreated control cells (red), treated cells (blue) and treated with indomethacin and A23187 cells (black).  Abbreviations: control cells (CTR), calcium ionophore (A23187, 5 µM) (CaI) and indomethacin (10 mM) (IND). ]]

Latest revision as of 13:10, 26 August 2019

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Return to 46BR.1N Fibroblasts

The aim of this study was to estimate the levels of AA in cells which were unstimulated and stimulated with A23187 (with/without COX inhibition), ATP and UVR. GC-FID was used for this fatty acid analysis and the values were normalized using cell numbers (ug/106 cells).

The estimated AA levels in 46BR.1N fibroblasts (unstimulated, A23187 (5µM), COX inhibited and A23187 (5 µM), ATP (2 mM) and UVR (15 mJ/cm2)) for 0.5, 1, 3 and 6h. Analysis was performed using GC-FID. Results are expressed mean ± SD (ug/106 cells) of three independent experiments.
Time (h) Experiment
Unstimulated A23187 Indomethacin + A23187 UVR ATP
0 1.13 ± 0.25 0.96 ± 0.00 0.64 ± 0.49 0.97 ± 0.46 1.00 ± 0.15
0.5 1.12 ± 0.27 0.44 ± 0.32 0.63 ± 0.15 0.78 ± 0.24 0.98 ± 0.13
1 1.09 ± 0.32 0.34 ± 0.13 0.84 ± 0.59 0.71 ± 0.18 0.98 ± 0.17
3 0.97 ± 0.19 0.51 ± 0.34 0.65 ± 0.13 0.58 ± 0.14 0.96 ± 0.12
6 1.03 ± 0.09 0.42 ± 0.23 0.79 ± 0.09 0.54 ± 0.04 1.03 ± 0.22


Levels of cellular arachidonic acid in 46BR.1N fibroblasts following stimulation with (A) A23187, (B) UVR and (C) ATP 6h post stimulation. Data points are from n=3 independent experiments. Non-linear lines of best fit are shown for untreated control cells (red), treated cells (blue) and treated with indomethacin and A23187 cells (black). Abbreviations: control cells (CTR), calcium ionophore (A23187, 5 µM) (CaI) and indomethacin (10 mM) (IND).