Difference between revisions of "Transformation of AA to 12-HPETE"

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(12-LOX Parameters)
(12-LOX Parameters)
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|Wild Type Leukocyte-Type 12-Lipoxygenase Expressed in E. coli.
 
|Wild Type Leukocyte-Type 12-Lipoxygenase Expressed in E. coli.
 
|<ref name="Richards1997"> [http://pubs.acs.org/doi/abs/10.1021/bi963051a Richards K. "Leukocyte 12-Lipoxygenase:  Expression, Purification, and Investigation of the Role of Methionine Residues in Turnover-Dependent Inactivation and 5,8,11,14-Eicosatetraynoic Acid Inhibition'' Biochemistry, 1997, 36 (22), pp 6692–6699]</ref>
 
|<ref name="Richards1997"> [http://pubs.acs.org/doi/abs/10.1021/bi963051a Richards K. "Leukocyte 12-Lipoxygenase:  Expression, Purification, and Investigation of the Role of Methionine Residues in Turnover-Dependent Inactivation and 5,8,11,14-Eicosatetraynoic Acid Inhibition'' Biochemistry, 1997, 36 (22), pp 6692–6699]</ref>
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{|class="wikitable sortable"
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|+  style="text-align: left;" | Enzyme Concentrations
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! Value
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! Species
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! Notes
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! Reference
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|5.00e-06 - 6.00e-05
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|mM
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|Human
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|Human reticulocyte 15-lipoxygenase-1, Enzymatic assays were performed in 25 mM Hepes buffer (pH 7.5,
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25C), with and without product (12-HETE) addition, in the presence of 25 μM of substrate.
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|<ref name="Wecksler2009"> [www.ncbi.nlm.nih.gov/pubmed/19469483 Wecksler A. "Mechanistic Investigations of Human Reticulocyte 15- and Platelet 12-Lipoxygenases with Arachidonic Acid'' Biochemistry, 2009, 48 (26), pp 6259–6267]</ref>
 
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Revision as of 10:00, 27 April 2016

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Reaction

R179AA - HPETE12.jpg

Chemical equation

 AA \rightleftharpoons PGH2

Rate equation

12-LOX Parameters

Michaelis-Menten Constants
Value Units Species Notes Reference
7.20E-03  mM Human Platelet [1]
8.00E-02  mM Human Platelet [2]
1.00E-02  mM Human Recombinant Histidine-Tagged Human Platelet 12-Lipoxygenase [3]
7.90E-03 ± 8.00E-04  mM Human Platelet 12-Lipoxygenase [4]
Enzyme Turnover Numbers
Value Units Species Notes Reference
336 ± 12 per minute Human Human reticulocyte 15-lipoxygenase-1, Enzymatic assays were performed in 25 mM Hepes buffer (pH 7.5,

25C), with and without product (12-HETE) addition, in the presence of 25 μM of substrate.

[5]
504 per minute Wild Boar Wild Type Leukocyte-Type 12-Lipoxygenase Expressed in E. coli. [6]
Enzyme Concentrations
Value Units Species Notes Reference
5.00e-06 - 6.00e-05 mM Human Human reticulocyte 15-lipoxygenase-1, Enzymatic assays were performed in 25 mM Hepes buffer (pH 7.5,

25C), with and without product (12-HETE) addition, in the presence of 25 μM of substrate.

[5]

References

Related Reactions